IFN-gamma Antibody (25723) [Alexa Fluor® 647] Summary
E. coli-derived recombinant human IFN-gamma
Accession # AAP20098.1
Detects human IFN-gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma, recombinant rat IFN-gamma, or recombinant porcine IFN-gamma is observed. Neutralizes the bioactivity of recombinant human IFN-gamma and will not neutralize the activity of rat or mouse IFN-gamma.
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Packaging, Storage & Formulations
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IFN-gamma Antibody (25723) [Alexa Fluor® 647]
- Immune interferon
- interferon gamma
- interferon, gamma
Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
- Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
- Pestka, S. et al. (2004) Immunol. Rev. 202:8.
- Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
- Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5401.
- Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
- Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
- McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
- Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
- Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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