Iduronate 2-Sulfatase/IDS Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Iduronate 2-Sulfatase/IDS Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Iduronate 2‑Sulfatase/IDS
  Thr36-Pro552
  Accession # Q08890
• Specificity: Detects mouse Iduronate 2‑Sulfatase/IDS in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human IDS is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IDS
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Iduronate 2-Sulfatase/IDS Antibody [Unconjugated]

• Alpha-L-iduronate sulfate sulfatase
• EC 3.1.6.13
• IDS
• iduronate 2-sulfatase 14 kDa chain
• iduronate 2-sulfatase 42 kDa chain
• iduronate 2-sulfatase
• Idursulfase
• MPS2
• S
• SIDS

Background

As a member of the sulfatase family, Iduronate 2-Sulfatase encoded by the IDS gene is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate (1, 2). It hydrolyzes the 2-sulfate group of the L-iduronate 2-sulfate units of the GAG. The IDS deficiency results in mucopolysaccharidosis II (MPS II or Hunter syndrome), an X-linked inborn error leading to lysosomal accumulation of the GAG and their excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe. The mouse IDS has sulfatase activity. In addition, recombinant human IDS is also available (Catalog # 2449-SU).

1. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
2. Neufeld, E.F. and J. Muenzer (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) p. 3421, New York, McGraw-Hill.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Iduronate 2-Sulfatase/IDS Antibody (AF2486)(1)

Publication using AF2486

• Eui-Cheol Jo Comparative study of idursulfase beta and idursulfase in vitro and in vivo J. Hum. Genet., 2016-11-10;0(0):. 2016-11-10 [PMID: 27829684] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IDS
• Uniprot:
  • Q08890()