Iduronate 2-Sulfatase/IDS Antibody [Unconjugated]

Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Iduronate 2-Sulfatase/IDS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2449) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Iduronate 2-Sulfatase/IDS at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Iduronate 2-Sulfatase/IDS Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IDS
  Ser26-Pro550
  Accession # P22304
• Specificity: Detects human IDS in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IDS is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IDS
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Iduronate 2-Sulfatase/IDS Antibody [Unconjugated]

• Alpha-L-iduronate sulfate sulfatase
• EC 3.1.6.13
• IDS
• iduronate 2-sulfatase 14 kDa chain
• iduronate 2-sulfatase 42 kDa chain
• iduronate 2-sulfatase
• idursulfase
• MPS2
• S
• SIDS

Background

As a member of the sulfatase family, IDS is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate (2, 3). It hydrolyzes the 2‑sulfate group of the L-iduronate 2-sulfate units of the GAG. The IDS deficiency results in mucopolysaccharidosis II (MPS II or Hunter syndrome), an X-linked inborn error leading to lysosomal accumulation of the GAG and its excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe. The deduced amino acid sequence of human IDS consists of a signal peptide (residues 1-25), a pro peptide (residues 26-33) and a mature chain (residues 34-550) that may be further processed into the 42 kDa chain (residues 34-455) and the 14 kDa chain (residues 456-550) (1). Recombinant human IDS corresponds to the single chain and has sulfatase activity described above.

1. Wilson, P.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8531.
2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
3. Neufeld, E.F. and J. Muenzer (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) pp. 3421-3452, New York, McGraw-Hill.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Iduronate 2-Sulfatase/IDS Antibody (AF2449)(6)

Publications using AF2449

• Annie Arguello, Cathal S. Mahon, Meredith E.K. Calvert, Darren Chan, Jason C. Dugas, Michelle E. Pizzo et al. Molecular architecture determines brain delivery of a transferrin receptor–targeted lysosomal enzyme Journal of Experimental Medicine 2022-03-07 [PMID: 35226042]
• Junjie Hong, Yu-Shan Cheng, Shu Yang, Manju Swaroop, Miao Xu, Jeanette Beers et al. iPS-derived neural stem cells for disease modeling and evaluation of therapeutics for mucopolysaccharidosis type II Experimental Cell Research 2022-03-01 [PMID: 34990619]
• A Arguello, R Meisner, ER Thomsen, HN Nguyen, R Ravi, J Simms, I Lo, J Speckart, J Holtzman, TM Gill, D Chan, Y Cheng, CL Chiu, JC Dugas, M Fang, IA Lopez, H Solanoy, B Tsogtbaata, Y Zhu, A Bhalla, KR Henne, AG Henry, A Delucchi, S Costanzo, JM Harris, D Diaz, K Scearce-Le, PE Sanchez Iduronate-2-sulfatase transport vehicle rescues behavioral and skeletal phenotypes in a mouse model of Hunter syndrome JCI Insight, 2021-10-08;6(19):. 2021-10-08 [PMID: 34622797] (ELISA Capture, Mouse)
• K Laoharawee, RC DeKelver, KM Podetz-Ped, M Rohde, S Sproul, HO Nguyen, T Nguyen, SJ St Martin, L Ou, S Tom, R Radeke, KE Meyer, MC Holmes, CB Whitley, T Wechsler, RS McIvor Dose-Dependent Prevention of Metabolic and Neurologic Disease in Murine MPS II by ZFN-Mediated In�Vivo Genome Editing Mol. Ther., 2018-03-10;26(4):1127-1136. 2018-03-10 [PMID: 29580682] (Western Blot, Mouse)
• Y Kondo, T Toyoda, R Ito, M Funato, Y Hosokawa, S Matsui, T Sudo, M Nakamura, C Okada, X Zhuang, A Watanabe, A Ohta, N Inagaki, K Osafune Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells Diabetologia, 2017-05-22;0(0):. 2017-05-22 [PMID: 28534195] (ICC, Human, Mouse)
• Araya K, Sakai N, Mohri I Localized donor cells in brain of a Hunter disease patient after cord blood stem cell transplantation. Mol. Genet. Metab., 2009-05-24;98(3):255-63. 2009-05-24 [PMID: 19556155] (Western Blot, Human)

Review for Iduronate 2-Sulfatase/IDS Antibody (AF2449) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 19602578
• Species: Rat

Bioinformatics

• Gene Symbol: IDS
• Uniprot:
  • P22304()