Iduronate 2-Sulfatase/IDS Antibody [Unconjugated]

Images

 
Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Iduronate 2‑Sulfatase/IDS Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Iduronate 2-Sulfatase/IDS Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IDS
Ser26-Pro550
Accession # P22304
Specificity
Detects human IDS in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IDS is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IDS
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunoprecipitation 25 ug/mL
Control
Iduronate 2-Sulfatase/IDS Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
AF2449 in the following applications:

Publications
Read Publications using
AF2449 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Iduronate 2-Sulfatase/IDS Antibody [Unconjugated]

  • Alpha-L-iduronate sulfate sulfatase
  • EC 3.1.6.13
  • IDS
  • iduronate 2-sulfatase 14 kDa chain
  • iduronate 2-sulfatase 42 kDa chain
  • iduronate 2-sulfatase
  • idursulfase
  • MPS2
  • S
  • SIDS

Background

As a member of the sulfatase family, IDS is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate (2, 3). It hydrolyzes the 2‑sulfate group of the L-iduronate 2-sulfate units of the GAG. The IDS deficiency results in mucopolysaccharidosis II (MPS II or Hunter syndrome), an X-linked inborn error leading to lysosomal accumulation of the GAG and its excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe. The deduced amino acid sequence of human IDS consists of a signal peptide (residues 1-25), a pro peptide (residues 26-33) and a mature chain (residues 34-550) that may be further processed into the 42 kDa chain (residues 34-455) and the 14 kDa chain (residues 456-550) (1). Recombinant human IDS corresponds to the single chain and has sulfatase activity described above.

  1. Wilson, P.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8531.
  2. Parenti, G. et al. (1997) Curr. Opin. Genet. & Dev. 7:386.
  3. Neufeld, E.F. and J. Muenzer (2001) in The Metabolic and Molecular Basis of Inherited Disease, Scriver, C.R. et al. (eds.) pp. 3421-3452, New York, McGraw-Hill.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP3-05061
Species: Hu, Mu
Applications: WB, ICC/IF
NBP1-85237
Species: Hu
Applications: IHC, IHC-Fr, IHC-P
NBP1-79280
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-56603
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P
NBP2-01770
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-236
Species: Hu, Mu
Applications: WB, IB, IHC, IHC-Fr, IP
NBP2-84403
Species: Hu
Applications: WB
NBP2-03621
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-30873
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, ChIP
H00004354-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP, S-ELISA
NBP1-54899
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr
H00284111-M06
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
NBP1-82604
Species: Hu
Applications: IHC, IHC-P
NBP1-84975
Species: Hu
Applications: IHC, IHC-P
MAB7745
Species: Hu
Applications: WB, Simple Western, IHC, KO

Publications for Iduronate 2-Sulfatase/IDS Antibody (AF2449)(3)

Review for Iduronate 2-Sulfatase/IDS Antibody (AF2449) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using AF2449:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Rat 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 19602578
SpeciesRat

Blocking

Blocking DetailsSee PMID 19602578

Details

Detection NotesSee PMID 19602578

Comments

CommentsPublished: See PMID 19602578

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Iduronate 2-Sulfatase/IDS Antibody (AF2449) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF2449

Additional Iduronate 2-Sulfatase/IDS Products

Bioinformatics Tool for Iduronate 2-Sulfatase/IDS Antibody (AF2449)

Discover related pathways, diseases and genes to Iduronate 2-Sulfatase/IDS Antibody (AF2449). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Iduronate 2-Sulfatase/IDS Antibody (AF2449)

Discover more about diseases related to Iduronate 2-Sulfatase/IDS Antibody (AF2449).
 

Pathways for Iduronate 2-Sulfatase/IDS Antibody (AF2449)

View related products by pathway.

PTMs for Iduronate 2-Sulfatase/IDS Antibody (AF2449)

Learn more about PTMs related to Iduronate 2-Sulfatase/IDS Antibody (AF2449).

Blogs on Iduronate 2-Sulfatase/IDS

There are no specific blogs for Iduronate 2-Sulfatase/IDS, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
01/07/2015
Application: WB
Species: Rat

Bioinformatics

Gene Symbol IDS
Uniprot