Iduronate 2-Sulfatase/IDS Antibody (OTI1D9)

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) [NBP2-01745] - Analysis of extracts (10ug) from a mouse cell line by using anti-IDS monoclonal antibody (1:200).

Immunocytochemistry/Immunofluorescence: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) [NBP2-01745] - Staining of COS7 cells.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - Staining of paraffin-embedded Human tonsil using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Iduronate 2 sulfatase antibody, and then analyzed by flow cytometry.

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Iduronate 2 sulfatase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Iduronate 2 sulfatase.

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) [NBP2-01745] - Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Iduronate 2 sulfatase monoclonal antibody.

Western Blot: Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) [NBP2-01745] - Analysis of extracts (10ug) from Human tissues by using anti-IDS monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - Iduronate 2 sulfatase Antibody (1D9) [NBP2-01745] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - Staining of paraffin-embedded Human lymphoma tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - Staining of paraffin-embedded Human pancreas tissue using anti-Iduronate 2 sulfatase mouse monoclonal antibody.

Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - Analysis of Hela cells, using anti-Iduronate 2 sulfatase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: Iduronate 2-Sulfatase/IDS Antibody (1D9) [NBP2-01745] - Analysis of Jurkat cells, using anti-Iduronate 2 sulfatase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Iduronate 2-Sulfatase/IDS Antibody (OTI1D9) Summary

• Immunogen: Full length human recombinant protein of human IDS(NP_000193) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IDS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 59.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Iduronate 2-Sulfatase/IDS Antibody (OTI1D9)

• Alpha-L-iduronate sulfate sulfatase
• EC 3.1.6.13
• IDS
• iduronate 2-sulfatase 14 kDa chain
• iduronate 2-sulfatase 42 kDa chain
• iduronate 2-sulfatase
• idursulfase
• MPS2
• S
• SIDS

Background

Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence similarity with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Iduronate 2-Sulfatase/IDS Antibody (NBP2-01745)(1)

Publication using NBP2-01745

• Hélène FE Gleitz, Ai Yin Liao, James R Cook, Samuel F Rowlston, Gabriella MA Forte, Zelpha D'Souza, Claire O'Leary, Rebecca J Holley, Brian W Bigger Brain‐targeted stem cell gene therapy corrects mucopolysaccharidosis type II via multiple mechanisms EMBO Molecular Medicine 2018-06-08 [PMID: 29884617]

Bioinformatics

• Gene Symbol: IDS
• Entrez:
  • 3423(Human)