IDI1 Antibody - BSA Free

Immunoprecipitation:IDI1 AntibodyNBP3-29684] -Detection of human IDI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IDI1 antibody NBP3-29684 (lot NBP3-29684-1) used for IP at 6 µg per reaction. IDI1 was also immunoprecipitated by rabbit anti-IDI1.. For blotting immunoprecipitated IDI1, NBP3-29684 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IDI1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IDI1 Antibody - BSA Free (NBP3-29684) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 177 and 227
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IDI1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for IDI1 Antibody - BSA Free

• EC 5.3.3.2
• IPP isomerase 1
• IPP1
• IPPI1
• isopentenyl diphosphate dimethylallyl diphosphate isomerase 1
• Isopentenyl pyrophosphate isomerase 1
• isopentenyl-diphosphate delta isomerase 1
• isopentenyl-diphosphate delta isomerase
• isopentenyl-diphosphate Delta-isomerase 1

Background

IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IDI1