IDH3B Antibody

Images

 
Western Blot: IDH3B Antibody [NBP2-14114] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: IDH3B Antibody [NBP2-14114] - Immunofluorescent staining of human cell line A549 shows localization to mitochondria.
Immunohistochemistry-Paraffin: IDH3B Antibody [NBP2-14114] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: IDH3B Antibody [NBP2-14114] - Staining of human colon shows strong cytoplasmic positivity in glandular cells and ganglion.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

IDH3B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVAIIGKIH TPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNN
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IDH3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2500-1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
IDH3B Protein (NBP2-14114PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for IDH3B Antibody

  • EC 1.1.1
  • EC 1.1.1.41
  • FLJ11043
  • H-IDHB
  • isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
  • isocitrate dehydrogenase 3 (NAD+) beta
  • isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, beta subunit
  • Isocitric dehydrogenase subunit beta
  • MGC903
  • NAD(+)-specific ICDH subunit beta
  • NAD+-specific ICDH
  • NAD+-specific isocitrate dehydrogenase b subunit
  • NAD+-specific isocitrate dehydrogenase beta
  • RP46

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IDH3B