IDH2 Antibody (673)

Western Blot: IDH2 Antibody (673) [NBP2-43572] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [673] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: IDH2 protein stained by IDH2 antibody [GT673] diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 um.

Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] - Mouse kidney [673] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug K562 whole cell lysate/extract D. 30 ug THP-1 whole cell lysate/extract E. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE IDH2 antibody dilution: 1:1000

Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug mouse brain lysate/extract 10 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000.

Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000

Western Blot: IDH2 Antibody (673) [NBP2-43572] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH2 antibody [T673].

Immunocytochemistry/Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - Analysis of HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: IDH2 protein stained by IDH2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] - Rat duodenum [673] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, RM, Xp
• Applications: WB, ICC/IF, IHC
• Clone: 673
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IDH2 Antibody (673) Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human IDH2. The exact sequence is proprietary.
• Predicted Species: Porcine (96%), Bovine (96%), Rhesus Macaque (100%), Chicken (95%), Xenopus (91%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IDH2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Xenopus laevis (91%), Zebrafish (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for IDH2 Antibody (673)

• D2HGA2
• EC 1.1.1.42
• ICD-M
• IDH
• IDHM
• IDP
• IDPM
• isocitrate dehydrogenase [NADP], mitochondrial
• isocitrate dehydrogenase 2 (NADP+), mitochondrial
• mNADP-IDH
• NADP(+)-specific ICDH
• Oxalosuccinate decarboxylase

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IDH2