IDH2 Antibody (673)

Images

 
Western Blot: IDH2 Antibody (673) [NBP2-43572] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [673] diluted ...read more
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: IDH2 protein stained by IDH2 antibody [GT673] diluted at 1:100.Blue: ...read more
Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] - Mouse kidney [673] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug K562 whole cell lysate/extract D. 30 ug THP-1 whole cell ...read more
Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug mouse brain lysate/extract 10 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000.
Western Blot: IDH2 Antibody (673) [NBP2-43572] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE IDH2 antibody [673] dilution: 1:1000
Genetic Strategies: Western Blot: IDH2 Antibody (673) [NBP2-43572] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody (673) [NBP2-43572] - Analysis of HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: IDH2 protein stained by IDH2 antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: IDH2 Antibody (673) [NBP2-43572] - Rat duodenum [673] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, RM, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
673
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

IDH2 Antibody (673) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human IDH2. The exact sequence is proprietary.
Predicted Species
Porcine (96%), Bovine (96%), Rhesus Macaque (100%), Chicken (95%), Xenopus (91%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IDH2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Xenopus laevis (91%), Zebrafish (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for IDH2 Antibody (673)

  • D2HGA2
  • EC 1.1.1.42
  • ICD-M
  • IDH
  • IDHM
  • IDP
  • IDPM
  • isocitrate dehydrogenase [NADP], mitochondrial
  • isocitrate dehydrogenase 2 (NADP+), mitochondrial
  • mNADP-IDH
  • NADP(+)-specific ICDH
  • Oxalosuccinate decarboxylase

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IDH2