ICOS Antibody

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139] - (2ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.

Immunohistochemistry-Paraffin: ICOS Antibody [NBP1-52139] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ICOS Antibody Summary

• Description: Novus Biologicals Goat ICOS Antibody (NBP1-52139) is a polyclonal antibody validated for use in IHC and ELISA. Anti-ICOS Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence KGGQILCDLTKTKGS corresponding to internal region according to NP_036224.1.
• Epitope: KGGQILCDLTKTKGS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ICOS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 13 kDa band in human peripheral blood Lymphocyte lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 22.6 kDa band according to NP_036224.1. The 13 kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: In paraffin embedded Human Lymph Node shows membrane staining just outside the mantle zone of the Germinal Centre.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ICOS Antibody

• Activation-inducible lymphocyte immunomediatory molecule
• AILIM
• AILIMCD278
• CD278 antigen
• CD278
• CRP-1
• CVID1
• ICOS
• inducible costimulator
• inducible T-cell costimulator
• inducible T-cell co-stimulator
• MGC39850

Background

CD278 is a 47-57 kD protein, also known as inducible costimulatory molecule (ICOS), or H4. This protein is homologous to the CD28/CTLA-4 proteins. It forms homodimers and is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. It has also been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICOS Antibody (NBP1-52139)(1)

Publication using NBP1-52139

• Strauss L, Bergmann C, Szczepanski MJ et al. Expression of ICOS on human melanoma-infiltrating CD4+CD25highFoxp3+ T regulatory cells: implications and impact on tumor-mediated immune suppression. J Immunol 2008-03-01 [PMID: 18292519]

Bioinformatics

• Gene Symbol: ICOS
• Entrez:
  • 29851(Human)
  • 54167(Mouse)
• Uniprot:
  • NP_036224.1(Human)