ICOS Antibody (669214) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with human ICOS was stained with Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB69753, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Ina functional Flow Cytometry test, biotinylated recombinant Human B7-H2 (Catalog #8206-B7, 5 ng/mL) binds to Human ICOS-transfectedHEK293 cells (black dotted line). Binding is completely blocked (filled histogram) by 2.5μg/mL of Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB69753). MouseIgG3 isotype (Catalog #MAB007) at 2.5 μg/mL was used as a control (blueline). Cells were stained with Sterptavidin-APC (Catalog # F0050).

In a Surface Plasmon Resonance test, Recombinant Human B7-H2(Catalog #8206-B7)coated at 30 ug/mL binds to Recombinant Human B7-H7 (Catalog #8084-B7,25 μg/mL (green line). Binding is completely blocked (orange line) by50 μg/mL of Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB69753). Non-blockinganti-ICOS antibody (Clone 669244) was used as control and did not affect ICOSbinding to B7-H2 (blue line).

• Reactivity: Hu
• Applications: Flow, B/N
• Clone: 669214
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ICOS Antibody (669214) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human ICOS and mouse myeloma cell line NS0-derived recombinant human ICOS cocktail
  Glu21-Phe141
  Accession # Q9Y6W8
• Specificity: Detects human ICOS in direct ELISAs.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICOS Antibody (669214) [Unconjugated]

• Activation-inducible lymphocyte immunomediatory molecule
• AILIM
• AILIMCD278
• CD278 antigen
• CD278
• CRP-1
• CVID1
• ICOS
• inducible costimulator
• inducible T-cell costimulator
• inducible T-cell co-stimulator
• MGC39850

Background

Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO⁺ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on T_h primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
2. Coyle, A.J. et al. (2000) Immunity 13:95.
3. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
5. Hutloff, A. et al. (1999) Nature 397:263.
6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9Y6W8()