ICAM-3/CD50 Antibody (ICAM3/1019)

Images

 
Western Blot: ICAM-3/CD50 Antibody (ICAM3/1019) [NBP2-44550] - Analysis of HL-60 Cell Lysate using CD50 Monoclonal Antibody (ICAM3/1019)
Immunohistochemistry-Paraffin: ICAM-3/CD50 Antibody (ICAM3/1019) [NBP2-44550] - Human Tonsil stained with CD50 Monoclonal Antibody (ICAM3/1019)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
ICAM3/1019
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

ICAM-3/CD50 Antibody (ICAM3/1019) Summary

Immunogen
Recombinant human ICAM-3/CD50 protein
Localization
Cell surface
Specificity
Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ICAM3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Western Blot 0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47903)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for ICAM-3/CD50 Antibody (ICAM3/1019)

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ICAM3
Uniprot