HYPE Antibody (3F5)

Western Blot: HYPE Antibody (3F5) [H00011153-M02] - Analysis of FICD expression in transfected 293T cell line by HYPE monoclonal antibody (M02), clone 3F5.Lane 1: FICD transfected lysate (Predicted MW: 51.8 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: HYPE Antibody (3F5) [H00011153-M02] - Detection limit for recombinant GST tagged FICD is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HYPE Antibody (3F5) Summary

• Immunogen: HYPE (AAH01342, 1 a.a. ~ 449 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAVTEPKWVSVWSRFLWVTLLSMVLGSLLALLLPLGAVEEQCLAVLKGLYLLRSKPDRAQHAATKCTSPSTELSITSRGATLLVAKTKASPAGKLEARAALNQALEMKRQGKREKAQKLFMHALKMDPDFVDALTEFGIFSEEDKDIIQADYLYTRALTISPYHEKALVNRDRTLPLVEEIDQRYFSIIDSKVKKVMSIPKGNSALRRVMEETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINTTLVSRIGSVTISDVLEIHRRVLGYVDPVEAGRFRTTQVLVGHHIPPHPQDVEKQMQEFVQWLNSEEAMNLHPVEFAALAHYKLVYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKEQRSDYYHVLEAANEGDVRPFIRFIAKCTETTLDTLLFATTEYSVALPEAQPNHSGFKETLPVKP
• Specificity: HYPE - Huntingtin interacting protein E (3F5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FICD
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HYPE Antibody (3F5)

• AMPylator FICD
• EC 2.7.7.n1
• FIC domain containing
• FIC domain-containing protein
• fic S-phase protein cell division homolog
• HIP-13
• HIP13UNQ3041
• huntingtin interacting protein 13
• Huntingtin interacting protein E
• huntingtin interactor protein E
• Huntingtin yeast partner E
• Huntingtin-interacting protein 13
• Huntingtin-interacting protein E
• HYPEadenosine monophosphate-protein transferase FICD
• MGC5623

Background

Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FICD
• Entrez:
  • 11153(Human)
• Uniprot:
  • AAH01342(Human)