HYPB Antibody

Images

 
Western Blot: HYPB Antibody [NB100-77334] - Analysis of mouse fibroblast lysates transiently expressing different siRNA in second and third lane (35 ug protein in RIPA buffer) using this antibody at 0.5 ug/mL. Primary ...read more
Flow Cytometry: HYPB Antibody [NB100-77334] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, PEP-ELISA, KD
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HYPB Antibody Summary

Immunogen
Peptide with sequence C-ERDPDKQTQNKE corresponding to internal region according to NP_054878.5.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SETD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/mL
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Knockdown Validated
Application Notes
Western blot validated for some lots. A customer has reported an approx. 290 kDa band observed in lysates of mouse fibroblasts. The observed band is knocked down by two different siRNA molecules. Calculated MW of 231 kDa band according to NP_054878.3). Data obtained from a previous batch (different goat). The Ponceau red demonstrates proper protein loading.
Control
HYPB Knockout HeLa Cell Lysate
HYPB Knockout 293T Cell Lysate
Publications
Read Publication using NB100-77334.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine. Mouse reactivity validated for some lots.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HYPB Antibody

  • FLJ22472
  • FLJ23184
  • FLJ45883
  • FLJ46217
  • HIF1
  • HIF-1EC 2.1.1.43
  • HIP-1
  • histone-lysine N-methyltransferase SETD2
  • hSET2
  • huntingtin interacting protein 1
  • Huntingtin yeast partner B
  • Huntingtin-interacting protein 1
  • Huntingtin-interacting protein B
  • HYPBp231HBP
  • KIAA1732SET domain-containing protein 2
  • KMT3AHBP231
  • Lysine N-methyltransferase 3A
  • SET domain containing 2
  • SET2FLJ16420

Background

Histone methylation plays an important role in eukaryotic transcriptional regulation. KMT3A / HYPB (huntingtin interacting protein B) also identified as HIF-1 (huntingtin interacting protein 1) is implicated in the pathogenesis of Huntington disease. KMT3A / HYPB coordinates histone methylation and transcriptional regulation in mammals. The HYPB domains, AWA-SET-PostSET, mediate histone H3 lysine 36 specific HMTase activity. The C-terminal of HYPB protein is important for its interaction with hyperphosphorylated RNA polymerase II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HYPB Antibody (NB100-77334)(1)

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SETD2
Entrez
Uniprot