Hydroxyacid Oxidase-1/HAO-1 Antibody (732410)

Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat Hydroxyacid Oxidase-1/HAO-1 Monoclonal Antibody (Catalog # MAB6197) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Hydroxyacid Oxidase-1/ HAO-1 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 732410
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Hydroxyacid Oxidase-1/HAO-1 Antibody (732410) Summary

• Immunogen: E. coli-derived recombinant human Hydroxyacid Oxidase-1/HAO-1
  Leu2-Ile370
  Accession # Q9UJM8
• Specificity: Detects human Hydroxyacid Oxidase-1/HAO-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HAO1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hydroxyacid Oxidase-1/HAO-1 Antibody (732410)

• (S)-2-hydroxy-acid oxidase
• EC 1.1.3.15
• Glycolate oxidase
• GOX
• GOX1MGC142227
• GOXMGC142225
• HAO1
• HAO-1
• HAOX1
• hydroxyacid oxidase (glycolate oxidase) 1
• hydroxyacid oxidase 1
• Hydroxyacid Oxidase1
• Hydroxyacid Oxidase-1

Background

Glycolate oxidase is a member of the superfamily of the alpha ‑hydroxy acid oxidases (HAO), enzymes that are present in both plants and animals (1). It catalyzes the FMN-mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide (2, 3). The co‑factor, FMN, is tightly bound but not covalently linked to the protein. In humans and other vertebrates, HAOs are found primarily in the peroxisomes of liver, kidney, and pancreas. Three HAOs have been identified in humans (4). HAO-1 is most highly expressed in liver and pancreas and is most active on two-carbon substrates such as glycolate. HAO-2 is expressed in liver and kidney and has greater activity against long-chain alpha ‑hydroxy acid substrates such as 2‑hydroxypalmitate. HAO-3 is expressed primarily in the pancreas. HAO contributes to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones (5).

1. Vignaud, C. et al. (2007) Arch. Biochem. Biophys. 465:410.
2. Murray, M.S. et al. (2008) Biochemistry, 47:2439.
3. Pennati, A. and G. Gadda. (2009) J. Biol. Chem. 284:31214.
4. Jones, J.M. et al. (2000) J. Biol. Chem. 275:12590.
5. Monico, G.C. et al. (2002) Kidney Int. 62:392.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAO1
• Uniprot:
  • Q9UJM8(Human)