HUS1B Antibody

Western Blot: HUS1B Antibody [NBP1-02812] - Analysis of HUS1B antibody in extracts from 293 and Hela cells

Immunohistochemistry-Paraffin: HUS1B Antibody [NBP1-02812] - Staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

HUS1B Antibody Summary

• Immunogen: Synthetic peptide corresponding to an internal region near aa 100-125 of human HUS1B.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HUS1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:5000
• Application Notes: IHC: NBP1-02812 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HUS1B Antibody

• checkpoint protein HUS1B
• hHUS1B
• HUS1 (S. pombe) checkpoint homolog b
• HUS1 checkpoint homolog b (S. pombe)
• MGC126746
• MGC126748
• RP11-532F6.1

Background

The protein encoded by the Hus1B gene is most closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage. This protein can interact with the checkpoint protein RAD1 but not with RAD9. Over expression of this protein has been shown to induce cell death, which suggests a related but distinct role of this protein, as compared to HUS1. Hus1B is expressed strongly in testis, less in spleen, thymus, prostate, colon and leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HUS1B
• Uniprot:
  • Q8NHY5(Human)