Humanin Antibody - BSA Free

Western Blot: Humanin Antibody [NB300-245] - 100ng of synthetic Humanin peptide and from 50 ug of total lysates of 293 T cells transfected with plasmids encoding GFPc1 or GFP-Humanin Conditions for the Western Blot: Lysis buffer: (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 20 mM EDTA, 50 mM NaF, 0.5% NP-40, 0.1 mM Na3VO4, 20 ug ml-1 leupeptin, 20 ug ml-1 aprotinin, 1 mM dithiothreitol and 1 mM phenylmethyl sulphonyl fluoride (PMSF)). Blocking buffer: (TRiS/Sodium chloride/buffer pH7.6) with 0.1% Tween-20, 5% skim Milk and 2% BSA). Humanin primary antibody(dilution 1/1000) and GFP antibody (dilution 1/2000) were incubated ON at 4 degrees C. Secondary rabbit HRP antibody was incubated during 1 hour at RT following by ECL detection.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Humanin Antibody - BSA Free Summary

• Immunogen: Residues 9-24 (LLLLTSEIDLPVKRRA) of the human Humanin protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MTRNR2L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: This antibody may be used for Western blot. No other assays have been tested. Suggested working dilutions: The investigator should determine the optimal working dilution for a specific application.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Humanin Antibody - BSA Free

• HN
• HUNIN

Background

A gene, Humanin, encodes a short polypeptide and abolishes death of neuronal cells caused by multiple different types of familial Alzheimer's disease genes and by Abeta amyloid, without effect on death by Q79 or superoxide dismutase-1 mutants. This polypeptide serves as a molecular clue for the development of new therapeutics for Alzheimer's disease targeting neuroprotection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MTRNR2L1