Reactivity | HuSpecies Glossary |
Applications | ELISA |
Conjugate | HRP |
Background | The Quantikine® Human VEGF R1/Flt-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF R1 in cell culture supernates, cell lysates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF R1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human VEGF R1 showed linear curves that were par...allel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human VEGF R1. Show More |
Specificity | Natural and recombinant human VEGF R1. |
Source | N/A |
Inter-Assay | See PDF Datasheet for details |
Intra-Assay | See PDF Datasheet for details |
Spike Recovery | See PDF Datasheet for details |
Sample Volume | See PDF Datasheet for details |
Dilutions |
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Application Notes | No significant interference observed with available related molecules. |
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Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.