Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit Summary

• Background: The Quantikine Human TIM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIM-1 in urine. It contains NS0-expressed recombinant human TIM-1 and antibodies raised against the recombinant factor. Natural human TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIM-1.
• Specificity: Natural and recombinant human TIM-1
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: HAVCR1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

T cell immunoglobulin and mucin domain 1 (TIM-1), also known as Kidney injury molecule 1 (KIM-1) and Hepatitis A virus cellular receptor 1 (HAVcr1), is a member of the TIM family which is involved in the regulation of innate and adaptive immune responses (1, 2). TIM-1 is a type I transmembrane protein that contains an N-terminal immunoglobulin-like domain, a mucin domain with O- and N-linked glycosylations, a transmembrane segment, and a cytoplasmic signaling domain (3, 4). Multiple TIM-1 variants can be produced due to polymorphisms or alternative splicing resulting in deletions in the mucin domain (3). Some of these polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans (5). Within the extracellular domain, human TIM-1 shares 41% amino acid sequence identity with mouse and rat TIM-1. 

In vivo, TIM-1 is expressed on splenic B cells and is a marker for the identification of IL-10+ regulatory B cells (6, 7). TIM-1 is also expressed on CD4+ T cells, mast cells, invariant NKT (iNKT) cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (4, 8-15). The expression of TIM-1 is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (4, 9, 13, 14, 16, 17). Metalloproteinase-mediated cleavage of TIM-1 at the membrane-proximal region results in the release of a soluble form of TIM-1 which is detectable in the urine and in circulation (18, 19). Urinary TIM-1 is highly elevated in nephropathy and may be a useful biomarker for renal damage (16, 20 - 25). 

TIM-1 has been reported to be a receptor for a number of ligands, including phosphatidylserine, leukocyte mono-immunoglobulin-like receptor 5 (LMIR5/CD300b), TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses (5, 15, 26-33). Its interaction with phosphatidylserine enables TIM-1 to mediate the phagocytosis of apoptotic cells (26-28). In TIM-1-bearing iNKT cells, interaction with apoptotic cells can also result in iNKT cell activation, proliferation, and cytokine production (11). Interactions between cell-surface or soluble TIM-1 with LMIR5 is proposed to induce LMIR5-mediated activation of myeloid cells including macrophages/monocytes, mast cells, neutrophils, and dendritic cells (29). These interactions contribute to tissue homeostasis and damage during kidney injury (29). Ligandinduced TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production (9, 31, 34). In humans, TIM-1 serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus and Marburgvirus (15, 33).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TIM-1/KIM-1/HAVCR (DKM100)(42)

Publications using DKM100

• Park, SI;Yu, U;Oh, WS;Ryu, SW;Son, S;Lee, S;Baek, H;Park, JI; Serum and urinary biomarkers of vancomycin-induced acute kidney injury: A prospective, observational analysis Medicine 2024-08-09 [PMID: 39121317] (Human)
• Kawakami, S;Yasuno, T;Kawakami, S;Ito, A;Fujimi, K;Matsuda, T;Nakashima, S;Masutani, K;Uehara, Y;Higaki, Y;Michishita, R; Effects of high-intensity intermittent exercise versus moderate-intensity continuous exercise on renal hemodynamics assessed by ultrasound echo Physiological reports 2024-01-01 [PMID: 38262710] (Human)
• Katz, DA;Gao, Z;Freytag, J;Mahendran, A;Szugye, C;Woodly, S;Alvarez, TCE;Lubert, AM;Alsaied, T;Goldstein, SL;Opotowsky, AR; Associations Between Characteristics of Individuals With Fontan Circulation With Blood and Urine Biomarkers of Kidney Injury and Dysfunction Journal of the American Heart Association 2023-06-22 [PMID: 37345835] (Human)
• Zhang, F;Yin, J;Liu, L;Liu, S;Zhang, G;Kong, Y;Wang, Y;Wang, N;Chen, X;Wang, F; IL-17C neutralization protects the kidney against acute injury and chronic injury EBioMedicine 2023-05-30 [PMID: 37263138] (Mouse)
• Downes, KJ;Zuppa, AF;Sharova, A;Neely, MN; Optimizing Vancomycin Therapy in Critically Ill Children: A Population Pharmacokinetics Study to Inform Vancomycin Area under the Curve Estimation Using Novel Biomarkers Pharmaceutics 2023-04-25 [PMID: 37242578] (Human)
• N Farooqui, M Zaidi, L Vaughan, TD McKee, E Ahsan, KD Pavelko, JC Villasboas, S Markovic, T Taner, N Leung, H Dong, MP Alexander, SM Herrmann Cytokines and Immune Cell Phenotype in Acute Kidney Injury Associated With Immune Checkpoint Inhibitors Kidney international reports, 2022-12-05;8(3):628-641. 2022-12-05 [PMID: 36938084] (Human)
• VC Wu, CK Chan, JS Chueh, YM Chen, YH Lin, CC Chang, PC Lin, SD Chung, TAIPAI gro Markers of Kidney Tubular Function Deteriorate While Those of Kidney Tubule Health Improve in Primary Aldosteronism After Targeted Treatments Journal of the American Heart Association, 2023-02-15;12(4):e028146. 2023-02-15 [PMID: 36789834] (Human)
• J Zhong, HC Yang, EL Shelton, T Matsusaka, AJ Clark, V Yermalitsk, Z Mashhadi, LS May-Zhang, MF Linton, AB Fogo, A Kirabo, SS Davies, V Kon Dicarbonyl-modified lipoproteins contribute to proteinuric kidney injury JCI Insight, 2022-11-08;7(21):. 2022-11-08 [PMID: 36125905] (Human)
• S Kawakami, T Yasuno, S Kawakami, A Ito, K Fujimi, T Matsuda, S Nakashima, K Masutani, Y Uehara, Y Higaki, R Michishita The moderate-intensity continuous exercise maintains renal blood flow and does not impair the renal function Physiological Reports, 2022-08-01;10(15):e15420. 2022-08-01 [PMID: 35924347] (Human)
• TJ Kakkanattu, J Kaur, V Nagesh, M Kundu, K Kamboj, P Kaur, J Sethi, HS Kohli, KL Gupta, A Ghosh, V Kumar, AK Yadav, V Jha Serum myo-inositol oxygenase levels at hospital discharge predict progression to chronic kidney disease in community-acquired acute kidney injury Scientific Reports, 2022-08-02;12(1):13225. 2022-08-02 [PMID: 35918463] (Human)
• R Upadhyay, V Batuman Aristolochic acid I induces proximal tubule injury through ROS/HMGB1/mt DNA mediated activation of TLRs Journal of Cellular and Molecular Medicine, 2022-06-28;0(0):. 2022-06-28 [PMID: 35765703] (Human)
• L Zeni, AGW Norden, E Prandi, C Canepa, K Burling, K Simpson, B Felappi, A Plebani, G Cancarini, PM Ferraro, D Fraser, RJ Unwin Exploration of a panel of urine biomarkers of kidney disease in two paediatric cohorts with Type 1 diabetes mellitus of differing duration Diabetology & metabolic syndrome, 2022-05-12;14(1):71. 2022-05-12 [PMID: 35550634] (Human)
• P Le?nik, E Wo?nica-Ni, J Janc, M Mierzcha?a, L ?ysenko Effect of a 3% gelatin solution on urinary KIM-1 levels in patients after thyroidectomy: a preliminary randomized controlled trial Scientific Reports, 2021-12-08;11(1):23617. 2021-12-08 [PMID: 34880372] (Human)
• Y Bignon, A Rinaldi, Z Nadour, V Poindessou, I Nemazanyy, O Lenoir, B Fohlen, P Weill-Rayn, A Hertig, A Karras, P Galichon, M Naesens, D Anglicheau, PE Cippà, N Pallet Cell stress response impairs de novo NAD+ biosynthesis in the kidney JCI Insight, 2022-01-11;7(1):. 2022-01-11 [PMID: 34793337] (Human)
• Yidan Zhang, Chunhong Xiang, Liying Gong, Yuanyuan Zhang, Junhui Zhen, Zhao Hu et al. Kidney injury molecule‑1 levels are associated with therapeutic outcomes and renal tubulointerstitial injury severity in idiopathic membranous nephropathy Experimental and Therapeutic Medicine 2021-10-11 [PMID: 34707715] (Human)
• J Rumpel, BJ Spray, VY Chock, MJ Kirkley, CL Slagle, A Frymoyer, SH Cho, KM Gist, R Blaszak, B Poindexter, SE Courtney Urine Biomarkers for the Assessment of Acute Kidney Injury in Neonates with Hypoxic Ischemic Encephalopathy Receiving Therapeutic Hypothermia The Journal of pediatrics, 2021-09-20;0(0):. 2021-09-20 [PMID: 34547334] (Human)
• MH Jacobson, Y Wu, M Liu, K Kannan, AJ Li, M Robinson, BA Warady, S Furth, H Trachtman, L Trasande Organophosphate pesticides and progression of chronic kidney disease among children: A prospective cohort study Environment international, 2021-05-02;155(0):106597. 2021-05-02 [PMID: 33951537] (Human)
• AM Østergaard, AN Jørgensen, S Bøvling, NP Ekeløf, FH Mose, JN Bech Effect of 0.9% NaCl compared to plasma-lyte on biomarkers of kidney injury, sodium excretion and tubular transport proteins in patients undergoing primary uncemented hip replacement - a randomized trial Bmc Nephrology, 2021-03-26;22(1):111. 2021-03-26 [PMID: 33771116] (Human)
• BE Funke, KE Jackson, WH Self, SP Collins, CT Saunders, L Wang, JD Blume, N Wickersham, RM Brown, JD Casey, GR Bernard, TW Rice, ED Siew, MW Semler, SMART Inve, Pragmatic Effect of balanced crystalloids versus saline on urinary biomarkers of acute kidney injury in critically ill adults Bmc Nephrology, 2021-02-05;22(1):54. 2021-02-05 [PMID: 33546622] (Human)
• E Latoch, K Kono?czuk, K Muszy?ska-, K Taranta-Ja, A Wasilewska, E Szymczak, J Trochim, M Krawczuk-R Urine NGAL and KIM-1-Tubular Injury Biomarkers in Long-Term Survivors of Childhood Solid Tumors: A Cross-Sectional Study Journal of Clinical Medicine, 2021-01-21;10(3):. 2021-01-21 [PMID: 33494327] (Human)
• MH Jacobson, Y Wu, M Liu, TM Attina, M Naidu, R Karthikraj, K Kannan, BA Warady, S Furth, S Vento, H Trachtman, L Trasande Serially assessed bisphenol A and phthalate exposure and association with kidney function in children with chronic kidney disease in the US and Canada: A longitudinal cohort study PLoS Med, 2020-10-14;17(10):e1003384. 2020-10-14 [PMID: 33052911] (Human)
• J Wajda, P Dumnicka, W Kolber, M Sporek, B Maziarz, P Ceranowicz, M Ku?niewski, B Ku?nierz-C The Marker of Tubular Injury, Kidney Injury Molecule-1 (KIM-1), in Acute Kidney Injury Complicating Acute Pancreatitis: A Preliminary Study J Clin Med, 2020-05-13;9(5):. 2020-05-13 [PMID: 32414176] (Human)
• M Brankovic, KM Akkerhuis, EJ Hoorn, N van Boven, JC van den Be, A Constantin, J Brugts, J van Ramsho, T Germans, H Hillege, E Boersma, V Umans, I Kardys Renal tubular damage and worsening renal function in chronic heart failure: Clinical determinants and relation to prognosis (Bio-SHiFT study) Clin Cardiol, 2020-04-16;0(0):. 2020-04-16 [PMID: 32298007] (Human)
• W Wo?yniec, K Kasprowicz, J Giebu?towi, N Korytowska, K Zorena, M Bartoszewi, P Rita-Tkach, M Renke, W Ratkowski Changes in Water Soluble Uremic Toxins and Urinary Acute Kidney Injury Biomarkers After 10- and 100-km Runs Int J Environ Res Public Health, 2019-10-28;16(21):. 2019-10-28 [PMID: 31661892] (Human)
• R Jouffroy, X Lebreton, N Mansencal, D Anglicheau Acute kidney injury during an ultra-distance race PLoS ONE, 2019-09-25;14(9):e0222544. 2019-09-25 [PMID: 31553742] (Human)
• T Gohda, N Kamei, T Koshida, M Kubota, K Tanaka, Y Yamashita, E Adachi, S Ichikawa, M Murakoshi, S Ueda, Y Suzuki Circulating kidney injury molecule-1 as a biomarker of renal parameters in diabetic kidney disease J Diabetes Investig, 2019-09-21;0(0):. 2019-09-21 [PMID: 31483944] (Human)
• Santelli A, Sun I, Eirin A, Abumoawad A, Woollard J, Lerman A, Textor S, Puranik A, Lerman L Senescent Kidney Cells in Hypertensive Patients Release Urinary Extracellular Vesicles. J Am Heart Assoc, 2019-06-01;8(11):e012584. 2019-06-01 [PMID: 31433703] (Human)
• Y Huang, Y Tian, S Likhodii, E Randell Baseline urinary KIM-1 concentration in detecting acute kidney injury should be interpreted with patient pre-existing nephropathy Pract Lab Med, 2019-03-07;15(0):e00118. 2019-03-07 [PMID: 30989103] (Human)
• D Kostic, GPNS Beozzo, SB do Couto, AHT Kato, L Lima, P Palmeira, VLJ Krebs, V Bunduki, RPV Francisco, M Zugaib, FT Dénes, WB de Carvalh, VHK Koch The role of renal biomarkers to predict the need of surgery in congenital urinary tract obstruction in infants J Pediatr Urol, 2019-03-16;0(0):. 2019-03-16 [PMID: 30979613] (Human)
• QQ Jiang, MF Han, K Ma, G Chen, XY Wan, SB Kilonzo, WY Wu, YL Wang, J You, Q Ning Acute kidney injury in acute-on-chronic liver failure is different from in decompensated cirrhosis World J. Gastroenterol., 2018-06-07;24(21):2300-2310. 2018-06-07 [PMID: 29881239] (Human)
• JJ Wang, NH Chi, TM Huang, R Connolly, LW Chen, SJ Chueh, WC Kan, CC Lai, VC Wu, JT Fang, TS Chu, KD Wu Urinary biomarkers predict advanced acute kidney injury after cardiovascular surgery Crit Care, 2018-04-26;22(1):108. 2018-04-26 [PMID: 29699579] (Human)
• SAE Dayem, AEME Bohy, M Hamed, S Ahmed Follow Up of Value of the Intrarenal Resistivity Indices and Different Renal Biomarkers for Early Identification of Diabetic Nephropathy in Type 1 Diabetic Patients Open Access Maced J Med Sci, 2017-03-20;5(2):188-192. 2017-03-20 [PMID: 28507626] (Human)
• Y Jin, X Shao, B Sun, C Miao, Z Li, Y Shi Urinary kidney injury molecule?1 as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method Mol Med Rep, 2017-01-05;0(0):. 2017-01-05 [PMID: 28075469] (Rat)
• Yiu W, Wong D, Wu H, Li R, Yam I, Chan L, Leung J, Lan H, Lai K, Tang S Kallistatin protects against diabetic nephropathy in db/db mice by suppressing AGE-RAGE-induced oxidative stress. Kidney Int, 2016-02-01;0(0):. 2016-02-01 [PMID: 26536000] (Human)
• Jongman R, van Klarenbosch J, Molema G, Zijlstra J, de Vries A, van Meurs M Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass. PLoS ONE, 2015-08-26;10(8):e0136205. 2015-08-26 [PMID: 26309217] (Human)
• Xu P, Wei L, Shang W, Tian S, Gu D, Yan T, Lin S Urinary kidney injury molecule-1 is related to pathologic involvement in IgA nephropathy with normotension, normal renal function and mild proteinuria. BMC Nephrol, 2014-07-07;15(0):107. 2014-07-07 [PMID: 24998891] (Human)
• Peake , Philip W, Pianta , Timothy, Succar , Lena, Fernando , Mangalee, Pugh , Debbie J, McNamara , Kathleen, Endre , Zoltan H A comparison of the ability of levels of urinary biomarker proteins and exosomal mRNA to predict outcomes after renal transplantation. PLoS ONE, 2014-06-11;9(2):e98644. 2014-06-11 [PMID: 24918752] (Human)
• Liu , Wei Jing, Luo , Mian-Na, Tan , Jin, Chen , Wei, Huang , Lei-zhao, Yang , Chen, Pan , Qingjun, Li , Benyi, Liu , Hua-feng Autophagy activation reduces renal tubular injury induced by urinary proteins. Autophagy, 2013-11-26;10(2):243-56. 2013-11-26 [PMID: 24345797] (Human)
• Urinary N-acetyl-beta-D glucosaminidase as a surrogate marker for renal function in autosomal dominant polycystic kidney disease: 1 year prospective cohort study. BMC Nephrol, 2012-08-30;13(0):93. 2012-08-30 [PMID: 22935351] (Human)
• Measuring urinary tubular biomarkers in type 2 diabetes does not add prognostic value beyond established risk factors. Kidney Int, 2012-06-20;82(7):812-8. 2012-06-20 [PMID: 22718188] (Human)
• de Borst MH, Nauta FL, Vogt L, Laverman GD, Gansevoort RT, Navis G Indomethacin reduces glomerular and tubular damage markers but not renal inflammation in chronic kidney disease patients: a post-hoc analysis. PLoS ONE, 2012-05-25;7(5):e37957. 2012-05-25 [PMID: 22662255] (Human)
• Metzger J, Kirsch T, Schiffer E, Ulger P, Mentes E, Brand K, Weissinger EM, Haubitz M, Mischak H, Herget-Rosenthal S Urinary excretion of twenty peptides forms an early and accurate diagnostic pattern of acute kidney injury. Kidney Int., 2010-09-08;78(12):1252-62. 2010-09-08 [PMID: 20827258] (Human)

Bioinformatics

• Gene Symbol: HAVCR1