Human UCH-L1/PGP9.5 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human UCH-L1/PGP9.5 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: UCHL1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human UCH-L1/PGP9.5 DuoSet ELISA, 5 Plate

• EC 3.4.19.12
• EC 6.-
• Neuron cytoplasmic protein 9.5
• PARK5
• PGP 9.5
• PGP9.5
• PGP9.5Uch-L1
• PGP95
• ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L1
• ubiquitin C-terminal hydrolase
• Ubiquitin thioesterase L1
• UCHL1
• UCH-L1

Background

Ubiquitin Carboxyl-terminal Esterase L1 (UCH-L1), also known as PGP9.5, is a deubiquitinating enzyme with a predicted molecular weight of 25 kDa. The human protein shares 95% amino acid sequence identity with its mouse and rat orthologs. UCH-L1 is expressed abundantly in neurons, accounting for 1-2% of total soluble proteins in the brain. It localizes primarily to the cytoplasm, but a subpopulation has been shown to be transiently nuclear. UCH-L1 contains two catalytic residues, Cys90 and His161, which are required for isopeptide bond cleavage at the C-terminal glycine residue of Ubiquitin. The levels of free Ubiquitin appear to be partially regulated by UCH-L1 through the hydrolysis of small Ubiquitin chains and the stabilization of monomeric Ubiquitin. Mice lacking functional UCH-L1 show neuronal dysfunction and neurodegeneration, and mutations in this enzyme have been linked to Parkinson's disease, suggesting that it is important for proper central nervous system function. UCH-L1 also likely plays a complex role in cancer. It has been reported to function as an oncogene in lymphoma, colorectal cancer, and nonsmall cell lung carcinoma. In contrast, it is thought to function as a tumor suppressor protein in nasopharyngeal and breast cancers.

Publications for UCH-L1/PGP9.5 (DY6007-05)(2)

Publications using DY6007-05

• Stacey, MJ;Leckie, T;Fitzpatrick, D;Hodgson, L;Barden, A;Jenkins, R;Galloway, R;Weller, C;Grivas, GV;Pitsiladis, Y;Richardson, AJ;Woods, DR; Neurobiomarker and body temperature responses to recreational marathon running Journal of science and medicine in sport 2023-09-21 [PMID: 37777396] (Human)
• K Sjölin, K Kultima, A Larsson, E Freyhult, C Zjukovskaj, K Alkass, J Burman Distribution of five clinically important neuroglial proteins in the human brain Molecular Brain, 2022-06-29;15(1):52. 2022-06-29 [PMID: 35765081] (Human)

FAQs for UCH-L1/PGP9.5 (DY6007-05). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: UCHL1