Human Total FGFR4 DuoSet IC ELISA, 2 Plate

Lysates prepared from MDA-MB-453 human breast cancer cells were serially diluted and analyzed by (A) IP-Western blot and (B) this DuoSet® IC ELISA. IPs were performed using an anti-FGF R4 monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-FGF R4 monoclonal antibody to detect total FGF R4. Bands were visualized by chemiluminescent detection. Human FGF R4 can be detected by this DuoSet IC ELISA by using approximately 2 to 4 times less lysate than is needed for a conventional IP-Western blot.

Lysates were prepared from MDA-MB-453 human breast cancer cells and HEK293 human embryonic kidney cells transfected with human FGF R4 (HEK293-hFGF R4). ELISA was performed using 100 μg of the MDA-MB-453 cell lysate and 5 μg of the HEK293-hFGF R4 cell lysate. IP-Western blot analyses were performed as described in Figure 1, using 400 μg of cell lysate.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Total FGFR4 DuoSet IC ELISA, 2 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FGFR4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total FGFR4 DuoSet IC ELISA, 2 Plate

• CD334 antigen
• CD334
• EC 2.7.10
• EC 2.7.10.1
• FGF R4
• FGFR4
• FGFR-4
• fibroblast growth factor receptor 4
• JTK2hydroxyaryl-protein kinase
• MGC20292
• protein-tyrosine kinase
• TKF
• tyrosine kinase related to fibroblast growth factor receptor
• tyrosylprotein kinase

Background

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Bioinformatics

• Gene Symbol: FGFR4