Human TGF-alpha Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human TGF-alpha Quantikine ELISA Kit Summary

• Background: The Quantikine Human TGF-alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TGF-alpha in cell culture supernates, serum, plasma, and human milk. It contains E. coli-expressed recombinant human TGF-alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TGF-alpha showed linear curves that were parallel to... the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TGF-alpha.
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• Specificity: Natural and recombinant human TGF-alpha
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TGFA

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TGF-alpha Quantikine ELISA Kit

• protransforming growth factor alpha
• TFGA
• TGFA
• TGFalpha
• TGF-alpha
• transforming growth factor, alpha
• transforming growth factor-alpha

Background

TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors with soluble forms released by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and plays a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. TGF-alpha binds and activates the EGF receptor. It induces fibroblast proliferation, epithelial development, keratinocyte migration, and angiogenesis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TGF-alpha (DTGA00)(29)

Publications using DTGA00

• Michalak, A;Guz, M;Kozicka, J;Cybulski, M;Jeleniewicz, W;Telejko, I;Szczygie?, K;Tywanek, E;Cicho?-Lach, H; miRNAs and Hematological Markers in Non-Alcoholic Fatty Liver Disease-A New Diagnostic Path? Biomedicines 2025-01-18 [PMID: 39857813] (Human)
• Michalak, A;Guz, M;Kozicka, J;Cybulski, M;Jeleniewicz, W;Szczygie?, K;Tywanek, E;Cicho?-Lach, H; microRNAs and Other Serological Markers of Liver Fibrosis in Patients with Alcohol-Related Liver Cirrhosis Biomedicines 2024-09-16 [PMID: 39335621] (Human)
• C Vogeley, NC Sondermann, S Woeste, AA Momin, V Gilardino, F Hartung, M Heinen, SK Maa beta , M Mescher, M Pollet, KM Rolfes, CFA Vogel, A Rossi, D Lang, ST Arold, M Nakamura, T Haarmann-S Unraveling the differential impact of PAHs and dioxin-like compounds on AKR1C3 reveals the EGFR extracellular domain as a critical determinant of the AHR response Environment international, 2021-11-20;158(0):106989. 2021-11-20 [PMID: 34991250] (Human)
• JKC Kre beta , C Jessen, A Marquardt, A Hufnagel, S Meierjohan NRF2 Enables EGFR Signaling in Melanoma Cells International Journal of Molecular Sciences, 2021-04-07;22(8):. 2021-04-07 [PMID: 33916908] (Human)
• A Michalak, H Cicho?-Lac, M Guz, J Kozicka, M Cybulski, W Jeleniewic Plateletcrit and Mean Platelet Volume in the Evaluation of Alcoholic Liver Cirrhosis and Nonalcoholic Fatty Liver Disease Patients BioMed Research International, 2021-02-15;2021(0):8867985. 2021-02-15 [PMID: 33644233] (Human)
• A Michalak, H Cicho?-Lac, M Guz, J Kozicka, M Cybulski, W Jeleniewic, A Stepulak Towards an evaluation of alcoholic liver cirrhosis and nonalcoholic fatty liver disease patients with hematological scales World Journal of Gastroenterology, 2020-12-21;26(47):7538-7549. 2020-12-21 [PMID: 33384553] (Human)
• J Codony-Ser, S García-Rom, MÁ Molina-Vil, J Bertran-Al, S Viteri, E d'Hondt, R Rosell Anti-epidermal growth factor vaccine antibodies increase the antitumor activity of kinase inhibitors in ALK and RET rearranged lung cancer cells Transl Oncol, 2020-10-23;14(1):100887. 2020-10-23 [PMID: 33129112] (Human)
• B Staumont, M Jamakhani, C Costa, F Vandermeer, SN Sriramared, G Redouté, C Mascaux, P Delvenne, P Hubert, R Safari, L Willems TGFalpha Promotes Chemoresistance of Malignant Pleural Mesothelioma Cancers (Basel), 2020-06-06;12(6):. 2020-06-06 [PMID: 32517259] (Human)
• P Mésange, A Bouygues, N Ferrand, M Sabbah, AE Escargueil, A Savina, B Chibaudel, C Tournigand, T Andre, A de Gramont, AK Larsen Combinations of Bevacizumab and Erlotinib show activity in colorectal cancer independent of RAS status Clin. Cancer Res., 2018-02-28;0(0):. 2018-02-28 [PMID: 29490990] (ELISA Capture, Human)
• D Alvarado, GF Ligon, JS Lillquist, SB Seibel, G Wallweber, VM Neumeister, DL Rimm, G McMahon, TM LaVallee ErbB activation signatures as potential biomarkers for anti-ErbB3 treatment in HNSCC PLoS ONE, 2017-07-19;12(7):e0181356. 2017-07-19 [PMID: 28723928] (Human)
• Chang S, Linderholm A, Harper R DUOX-Mediated Signaling Is Not Required for LPS-Induced Neutrophilic Response in the Airways. PLoS ONE, 2015-07-06;10(7):e0131810. 2015-07-06 [PMID: 26148206] (Mouse)
• Yonesaka , Kimio, Satoh , Taroh, Ueda , Shinya, Yoshida , Takeshi, Takeda , Masayuki, Shimizu , Toshio, Okamoto , Isamu, Nishio , Kazuto, Tamura , Takao, Nakagawa , Kazuhiko Circulating hepatocyte growth factor is correlated with resistance to cetuximab in metastatic colorectal cancer. Anticancer Res, 2015-03-01;35(3):1683-9. 2015-03-01 [PMID: 25750328] (Human)
• Zhang Z, Dong Z, Lauxen I, Filho M, Nor J Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype. Cancer Res, 2014-03-31;74(10):2869-81. 2014-03-31 [PMID: 24686166] (Human)
• Tanizaki J, Okamoto I, Okabe T, Sakai K, Tanaka K, Hayashi H, Kaneda H, Takezawa K, Kuwata K, Yamaguchi H, Hatashita E, Nishio K, Nakagawa K Activation of HER family signaling as a mechanism of acquired resistance to ALK inhibitors in EML4-ALK-positive non-small cell lung cancer. Clin Cancer Res, 2012-07-27;18(22):6219-26. 2012-07-27 [PMID: 22843788] (Human)
• Holterman CE, Franovic A, Payette J ETS-1 oncogenic activity mediated by transforming growth factor alpha. Cancer Res., 2010-01-12;70(2):730-40. 2010-01-12 [PMID: 20068146] (Human)
• Egloff AM, Rothstein ME, Seethala R, Siegfried JM, Grandis JR, Stabile LP Cross-talk between estrogen receptor and epidermal growth factor receptor in head and neck squamous cell carcinoma. Clin. Cancer Res., 2009-10-13;15(21):6529-40. 2009-10-13 [PMID: 19825947] (Human)
• Kreeger PK, Mandhana R, Alford SK, Haigis KM, Lauffenburger DA RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects. Cancer Res., 2009-09-29;69(20):8191-9. 2009-09-29 [PMID: 19789336] (Human)
• Debucquoy A, Haustermans K, Daemen A, Aydin S, Libbrecht L, Gevaert O, De Moor B, Tejpar S, McBride WH, Penninckx F, Scalliet P, Stroh C, Vlassak S, Sempoux C, Machiels JP Molecular response to cetuximab and efficacy of preoperative cetuximab-based chemoradiation in rectal cancer. J. Clin. Oncol., 2009-03-30;27(17):2751-7. 2009-03-30 [PMID: 19332731] (Human)
• Han SW, Oh DY, Im SA, Park SR, Lee KW, Song HS, Lee NS, Lee KH, Choi IS, Lee MH, Kim MA, Kim WH, Bang YJ, Kim TY Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer. Br. J. Cancer, 2009-01-06;100(2):298-304. 2009-01-06 [PMID: 19127259] (Human)
• Bergin DA, Greene CM, Sterchi EE, Kenna C, Geraghty P, Belaaouaj A, Taggart CC, O'Neill SJ, McElvaney NG Activation of the epidermal growth factor receptor (EGFR) by a novel metalloprotease pathway. J. Biol. Chem., 2008-09-04;283(46):31736-44. 2008-09-04 [PMID: 18772136] (Human)
• Sasaki T, Nakamura T, Rebhun RB, Cheng H, Hale KS, Tsan RZ, Fidler IJ, Langley RR Modification of the primary tumor microenvironment by transforming growth factor alpha-epidermal growth factor receptor signaling promotes metastasis in an orthotopic colon cancer model. Am. J. Pathol., 2008-07-01;173(1):205-16. 2008-07-01 [PMID: 18583324] (Human)
• Ren W, Korchin B, Zhu QS, Wei C, Dicker A, Heymach J, Lazar A, Pollock RE, Lev D Epidermal Growth Factor Receptor Blockade in Combination with Conventional Chemotherapy Inhibits Soft Tissue Sarcoma Cell Growth In vitro and In vivo. Clin. Cancer Res., 2008-05-01;14(9):2785-2795. 2008-05-01 [PMID: 18451246] (Human)
• Le Quement C, Guenon I, Gillon JY, Lagente V, Boichot E MMP-12 induces IL-8/CXCL8 secretion through EGFR and ERK1/2 activation in epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2008-04-04;294(6):L1076-84. 2008-04-04 [PMID: 18390828] (Human)
• Garcia B, Neninger E, de la Torre A, Leonard I, Martinez R, Viada C, Gonzalez G, Mazorra Z, Lage A, Crombet T Effective inhibition of the epidermal growth factor/epidermal growth factor receptor binding by anti-epidermal growth factor antibodies is related to better survival in advanced non-small-cell lung cancer patients treated with the epidermal growth factor cancer vaccine. Clin. Cancer Res., 2008-02-01;14(3):840-6. 2008-02-01 [PMID: 18245547] (Human)
• Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases. J. Biol. Chem., 2007-07-17;282(37):26656-64. 2007-07-17 [PMID: 17636266] (Mouse)
• Lemos-Gonzalez Y, Rodriguez-Berrocal FJ, Cordero OJ, Gomez C, Paez de la Cadena M Alteration of the serum levels of the epidermal growth factor receptor and its ligands in patients with non-small cell lung cancer and head and neck carcinoma. Br. J. Cancer, 2007-04-24;96(10):1569-78. 2007-04-24 [PMID: 17453000] (Human)
• Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK The response of human epithelial cells to TNF involves an inducible autocrine cascade. Cell, 2006-03-24;124(6):1225-39. 2006-03-24 [PMID: 16564013] (Human)
• Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J. Immunol., 2005-11-01;175(9):6177-89. 2005-11-01 [PMID: 16237115] (Human)
• Scholl FA, Dumesic PA Mek1 alters epidermal growth and differentiation. Cancer Res., 2004-09-01;64(17):6035-40. 2004-09-01 [PMID: 15342384] (Porcine)

Bioinformatics

• Gene Symbol: TGFA