Human TGF-alpha DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human TGF-alpha DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TGFA

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TGF-alpha DuoSet ELISA, 15 Plate

• protransforming growth factor alpha
• TFGA
• TGFA
• TGFalpha
• TGF-alpha
• transforming growth factor, alpha
• transforming growth factor-alpha

Background

TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors with soluble forms released by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and plays a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. TGF-alpha binds and activates the EGF receptor. It induces fibroblast proliferation, epithelial development, keratinocyte migration, and angiogenesis.

Publications for TGF-alpha (DY239)(26)

Publications using DY239

• Arshad, M;Azad, A;Chan, PYK;Vigneswara, V;Feldinger, K;Nafi, SNM;Laporte-Maguire, E;De Santo, C;Zuo, J;Shaaban, AM;Kong, A; Neratinib could be effective as monotherapy or in combination with trastuzumab in HER2-low breast cancer cells and organoid models British journal of cancer 2024-04-10 [PMID: 38600326] (Human)
• R Fang, C Haxaire, M Otero, S Lessard, G Weskamp, DR McIlwain, TW Mak, SF Lichtentha, CP Blobel Role of iRhoms 1 and 2 in Endochondral Ossification Int J Mol Sci, 2020-11-19;21(22):. 2020-11-19 [PMID: 33227998] (Human)
• SJ Wang, R Li, TSC Ng, G Luthria, MJ Oudin, M Prytyskach, RH Kohler, R Weissleder, DA Lauffenbur, MA Miller Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy Sci Adv, 2020-05-22;6(21):eaaz8521. 2020-05-22 [PMID: 32494745] (Human)
• A Sampera, FJ Sánchez-Ma, O Arpí, L Visa, M Iglesias, S Menéndez, É Gaye, A Dalmases, S Clavé, M Gelabert-B, TT Poulsen, M Kragh, B Bellosillo, J Albanell, A Rovira, C Montagut HER-family ligands promote acquired resistance to trastuzumab in gastric cancer Mol. Cancer Ther., 2019-09-04;0(0):. 2019-09-04 [PMID: 31484705] (Human)
• J Li, TR Bai, S Gao, Z Zhou, XM Peng, LS Zhang, DL Dou, ZS Zhang, LY Li Human rhomboid family-1 modulates clathrin coated vesicle-dependent pro-transforming growth factor ? membrane trafficking to promote breast cancer progression EBioMedicine, 2018-09-29;0(0):. 2018-09-29 [PMID: 30279141] (Human)
• P Arcidiacon, CM Webb, MA Brooke, H Zhou, PJ Delaney, KE Ng, DC Blaydon, A Tinker, DP Kelsell, A Chikh p63 is a key regulator of iRHOM2 signalling in the keratinocyte stress response Nat Commun, 2018-03-09;9(1):1021. 2018-03-09 [PMID: 29523849] (Human)
• JL Wilson, E Kefalogian, L Stopfer, C Harrison, VS Sabbisetti, E Fraenkel, DA Lauffenbur, A Herrlich Functional Genomics Approach Identifies Novel Signaling Regulators of TGF? Ectodomain Shedding Mol. Cancer Res., 2017-10-10;0(0):. 2017-10-10 [PMID: 29018056] (Human)
• Y Miura, Y Sukawa, S Hironaka, M Mori, K Nishikawa, S Tokunaga, H Okuda, T Sakamoto, K Taku, K Nishikawa, T Moriwaki, Y Negoro, Y Kimura, K Uchino, K Shinozaki, H Shinozaki, N Musha, H Yoshiyama, T Tsuda, Y Miyata, N Sugimoto, T Shirakawa, M Ito, K Yonesaka, K Yoshimura, N Boku, K Nosho, T Takano, I Hyodo Five-weekly S-1 plus cisplatin therapy combined with trastuzumab therapy in HER2-positive gastric cancer: a phase II trial and biomarker study (WJOG7212G) Gastric Cancer, 2017-05-11;0(0):. 2017-05-11 [PMID: 28497176] (Human)
• Seyyed Mortaza Haghgoo Prognostic value of rare and complex mutations in EGFR and serum levels of soluble EGFR and its ligands in Iranian non-small cell lung carcinoma patients Clin. Biochem, 2016-12-05;0(0):. 2016-12-05 [PMID: 27923629] (Human)
• ADAM17 substrate release in proximal tubule drives kidney fibrosis JCI Insight, 2016-08-18;1(13):. 2016-08-18 [PMID: 27642633] (Human)
• Zhang C, Spevak W, Zhang Y, Burton E, Ma Y, Habets G, Zhang J, Lin J, Ewing T, Matusow B, Tsang G, Marimuthu A, Cho H, Wu G, Wang W, Fong D, Nguyen H, Shi S, Womack P, Nespi M, Shellooe R, Carias H, Powell B, Light E, Sanftner L, Walters J, Tsai J, West B, Visor G, Rezaei H, Lin P, Nolop K, Ibrahim P, Hirth P, Bollag G RAF inhibitors that evade paradoxical MAPK pathway activation. Nature, 2015-10-14;526(7574):583-6. 2015-10-14 [PMID: 26466569] (Mouse)
• Sarah Louise Dombernowsky, Jacob Samsøe-Petersen, Camilla Hansson Petersen, Rachael Instrell, Anne-Mette Bornhardt Hedegaard, Laurel Thomas et al. The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17 Nature Communications 2015-06-25 [PMID: 26108729] (Human)
• Dreymueller D, Martin C, Schumacher J, Groth E, Boehm J, Reiss L, Uhlig S, Ludwig A Smooth muscle cells relay acute pulmonary inflammation via distinct ADAM17/ErbB axes. J Immunol, 2013-12-16;192(2):722-31. 2013-12-16 [PMID: 24342803] (Human)
• Breshears L, Schlievert P, Peterson M A disintegrin and metalloproteinase 17 (ADAM17) and epidermal growth factor receptor (EGFR) signaling drive the epithelial response to Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1). J Biol Chem, 2012-07-25;287(39):32578-87. 2012-07-25 [PMID: 22833676] (Human)
• Richards FM, Tape CJ, Jodrell DI, Murphy G Anti-Tumour Effects of a Specific Anti-ADAM17 Antibody in an Ovarian Cancer Model In Vivo. PLoS ONE, 2012-07-11;7(7):e40597. 2012-07-11 [PMID: 22792380] (Human)
• Inoue A, Arima N, Ishiguro J, Prestwich GD, Arai H, Aoki J LPA-producing enzyme PA-PLA(1)alpha regulates hair follicle development by modulating EGFR signalling. EMBO J., 2011-08-19;30(20):4248-60. 2011-08-19 [PMID: 21857648] (Mouse)
• Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Clin. Cancer Res., 2011-04-11;17(11):3619-30. 2011-04-11 [PMID: 21482691] (Human)
• Murthy A, Defamie V, Smookler DS Ectodomain shedding of EGFR ligands and TNFR1 dictates hepatocyte apoptosis during fulminant hepatitis in mice. J. Clin. Invest., 2010-07-12;120(8):2731-44. 2010-07-12 [PMID: 20628198] (Mouse)
• Hickinson DM, Marshall GB, Beran GJ Identification of biomarkers in human head and neck tumor cell lines that predict for in vitro sensitivity to gefitinib. Clin Transl Sci, 2009-06-01;2(3):183-92. 2009-06-01 [PMID: 20443891] (Human)
• Inoue Y, Miyamoto S, Fukami T, Shirota K, Yotsumoto F, Kawarabayashi T Amphiregulin is much more abundantly expressed than transforming growth factor-alpha and epidermal growth factor in human follicular fluid obtained from patients undergoing in vitro fertilization-embryo transfer. Fertil. Steril., 2008-03-05;91(4):1035-41. 2008-03-05 [PMID: 18325497] (Human)
• Yotsumoto F, Yagi H, Suzuki SO, Oki E, Tsujioka H, Hachisuga T, Sonoda K, Kawarabayashi T, Mekada E, Miyamoto S Validation of HB-EGF and amphiregulin as targets for human cancer therapy. Biochem. Biophys. Res. Commun., 2007-11-20;365(3):555-61. 2007-11-20 [PMID: 18023415] (Human)
• Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components. Eur. Respir. J., 2007-09-05;30(6):1064-73. 2007-09-05 [PMID: 17804444] (Human)
• Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer. Cancer Cell, 2006-07-01;10(1):39-50. 2006-07-01 [PMID: 16843264] (Human)
• Sakai K, Yokote H, Murakami-Murofushi K, Tamura T, Saijo N, Nishio K In-frame deletion in the EGF receptor alters kinase inhibition by gefitinib. Biochem. J., 2006-08-01;397(3):537-43. 2006-08-01 [PMID: 16623663] (Human)
• Baginski TK, Dabbagh K, Satjawatcharaphong C, Swinney DC Cigarette smoke synergistically enhances respiratory mucin induction by proinflammatory stimuli. Am. J. Respir. Cell Mol. Biol., 2006-03-16;35(2):165-74. 2006-03-16 [PMID: 16543607] (Human)
• Miyamoto S, Hirata M, Yamazaki A, Kageyama T, Hasuwa H, Mizushima H, Tanaka Y, Yagi H, Sonoda K, Kai M, Kanoh H, Nakano H, Mekada E Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy. Cancer Res., 2004-08-15;64(16):5720-7. 2004-08-15 [PMID: 15313912] (Human)

FAQs for TGF-alpha (DY239). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TGFA