Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit Summary

• Background: The Quantikine Human S100A8/S100A9 Heterodimer Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human S100A8/S100A9 Heterodimer in cell culture supernates, tissue lysastes, serum, plasma, saliva, urine, human milk, and fecal extract. It contains E. coli-expressed recombinant human S100A8/S100A9 Heterodimer and antibodies raised against the recombinant factor. Results ...obtained using natural human S100A8/S100A9 Heterodimer showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human S100A8/S100A9 Heterodimer.
  Show More
• Specificity: Natural and recombinant human S100A8/S100A9 Heterodimer.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: S100A8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human S100A8/S100A9 Heterodimer Quantikine ELISA Kit

• 60B8AG
• CAGA
• calgranulin A
• Calprotectin L1L subunit
• Calprotectin
• CFAG
• CGLA
• CP-10
• Cystic fibrosis antigen
• L1AG
• Leukocyte L1 complex light chain
• MA387
• MIF
• Migration inhibitory factor-related protein 8
• MRP8
• NIF
• p8
• protein S100-A8
• S100-A8
• S100A8/S100A9 Heterodimer
• Urinary stone protein band A

Background

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca²⁺-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca²⁺-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for S100A8/S100A9 Heterodimer (DS8900)(15)

Publications using DS8900

• Pons, S;Trouillet-Assant, S;Subtil, F;Abbas-Chorfa, F;Cornaton, E;Berthiot, A;Galletti, S;Plat, A;Rapin, S;Trapes, L;Generenaz, L;Brengel-Pesce, K;Callies, A;Plaisant, F;Claris, O;Portefaix, A;Flamant, C;Butin, M; Performance of 11 Host Biomarkers Alone or in Combination in the Diagnosis of Late-Onset Sepsis in Hospitalized Neonates: The Prospective EMERAUDE Study Biomedicines 2023-06-13 [PMID: 37371798] (Human)
• F Real, A Zhu, B Huang, A Belmellat, A Sennepin, T Vogl, C Ransy, M Revol, R Arrigucci, A Lombès, J Roth, ML Gennaro, F Bouillaud, S Cristofari, M Bomsel S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo Nature Communications, 2022-10-11;13(1):5956. 2022-10-11 [PMID: 36220814] (Human)
• Z Li, O McGinn, Y Wu, A Bahreini, NM Priedigkei, K Ding, S Onkar, C Lampenfeld, CA Sartorius, L Miller, M Rosenzweig, O Cohen, N Wagle, JK Richer, WJ Muller, L Buluwela, S Ali, TC Bruno, DAA Vignali, Y Fang, L Zhu, GC Tseng, J Gertz, JM Atkinson, AV Lee, S Oesterreic ESR1 mutant breast cancers show elevated basal cytokeratins and immune activation Nature Communications, 2022-04-19;13(1):2011. 2022-04-19 [PMID: 35440136] (Human)
• AV Sen'kova, IA Savin, EV Brenner, MA Zenkova, AV Markov Core genes involved in the regulation of acute lung injury and their association with COVID-19 and tumor progression: A bioinformatics and experimental study PLoS ONE, 2021-11-22;16(11):e0260450. 2021-11-22 [PMID: 34807957] (Human)
• GA Kinney, EN Haddad, LS Garrow, PKW Ng, SS Comstock An Intervention With Michigan-Grown Wheat in Healthy Adult Humans to Determine Effect on Gut Microbiota: Protocol for a Crossover Trial JMIR research protocols, 2021-10-06;10(10):e29046. 2021-10-06 [PMID: 34612840] (Human)
• IA Drosatos, JN Tsoporis, S Izhar, S Gupta, G Tsirebolos, E Sakadakis, AS Triantafyl, A Rigopoulos, D Rigopoulos, LS Rallidis, I Rizos, TG Parker Differential Regulation of Circulating Soluble Receptor for Advanced Glycation End Products (sRAGEs) and Its Ligands S100A8/A9 Four Weeks Post an Exercise Intervention in a Cohort of Young Army Recruits Biomolecules, 2021-09-13;11(9):. 2021-09-13 [PMID: 34572568] (Human)
• S Buhelt, HB Søndergaar, MR Mahler, S Cobanovic, L Börnsen, C Ammitzbøll, AB Oturai, F Sellebjerg Biomarkers of systemic inflammation, soluble IL-2Ralpha and the multiple sclerosis-associated IL2RA SNP rs2104286 in healthy subjects and multiple sclerosis patients Multiple sclerosis and related disorders, 2021-07-07;54(0):103140. 2021-07-07 [PMID: 34304016] (Human)
• A Olsson, S Gustavsen, AR Langkilde, TH Hansen, F Sellebjerg, H Bach Sønde, AB Oturai Circulating levels of tight junction proteins in multiple sclerosis: Association with inflammation and disease activity before and after disease modifying therapy Multiple sclerosis and related disorders, 2021-07-03;54(0):103136. 2021-07-03 [PMID: 34247104] (Human)
• A Olsson, S Gustavsen, IC Hasselbalc, AR Langkilde, F Sellebjerg, AB Oturai, HB Søndergaar Biomarkers of inflammation and epithelial barrier function in multiple sclerosis Mult Scler Relat Disord, 2020-09-19;46(0):102520. 2020-09-19 [PMID: 32980645] (Human)
• DG Bisson, K Sheng, S Kocabas, E Krock, A Teles, N Saran, JA Ouellet, L Haglund Toll-like receptor involvement in adolescent scoliotic facet joint degeneration J. Cell. Mol. Med., 2020-08-27;0(0):. 2020-08-27 [PMID: 32853438] (Human)
• DR Shores, J Fundora, M Go, F Shakeel, S Brooks, SM Alaish, J Yang, CP Sodhi, DJ Hackam, A Everett Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages BMC Pediatr, 2020-05-26;20(1):250. 2020-05-26 [PMID: 32456678] (Human)
• A Grand, E Rochette, F Dutheil, D Gozal, V Calcaterra, R Berni Cana, N Cobanoglu, JPM Derikx, G Terrin, B Pereira, E Merlin Body Mass Index and Calprotectin Blood Level Correlation in Healthy Children: An Individual Patient Data Meta-Analysis J Clin Med, 2020-03-20;9(3):. 2020-03-20 [PMID: 32245056] (Human)
• RD Williamson, FP McCarthy, LC Kenny, CM McCarthy Activation of a TLR9 mediated innate immune response in preeclampsia Sci Rep, 2019-04-11;9(1):5920. 2019-04-11 [PMID: 30976066] (Human)
• CJ Popp, DE St-Jules, L Hu, L Ganguzza, P Illiano, M Curran, H Li, A Schoenthal, M Bergman, AM Schmidt, E Segal, A Godneva, MA Sevick The rationale and design of the personal diet study, a randomized clinical trial evaluating a personalized approach to weight loss in individuals with pre-diabetes and early-stage type 2 diabetes Contemp Clin Trials, 2019-03-04;0(0):. 2019-03-04 [PMID: 30844471] (Human)
• Dietary zinc alters the microbiota and decreases resistance to Clostridium difficile infection Nat Med, 2016-09-26;0(0):. 2016-09-26 [PMID: 27668938] (Human)

Bioinformatics

• Gene Symbol: S100A8