Human S100A8 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human S100A8 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: S100A8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human S100A8 DuoSet ELISA, 5 Plate

• 60B8AG
• CAGACP-10
• Calgranulin A
• calgranulin-A
• Calprotectin L1L subunit
• CFAG
• CFAGL1Ag
• CGLA
• Cystic fibrosis antigen
• Leukocyte L1 complex light chain
• MA387
• MIF
• Migration inhibitory factor-related protein 8
• MRP8
• MRP-8
• MRP8S100 calcium binding protein A8 (calgranulin A)
• NIF
• p8
• protein S100-A8
• S100 calcium binding protein A8
• S100 calcium-binding protein A8 (calgranulin A)
• S100 calcium-binding protein A8
• S100A8
• Urinary stone protein band A

Background

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca²⁺-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca²⁺-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Publications for S100A8 (DY4570-05)(13)

Publications using DY4570-05

• Cho, HY;Lee, JE;Park, KH;Choi, BY;Lee, MJ;Jeong, DE;Shin, S; Identification and characterization of plasma proteins associated with intra-amniotic inflammation and/or infection in women with preterm labor. Scientific reports 2024-06-25 [PMID: 38918423] (Human)
• Chick, HM;Rees, ME;Lewis, ML;Williams, LK;Bodger, O;Harris, LG;Rushton, S;Wilkinson, TS; Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses Biomedicines 2024-03-25 [PMID: 38672079] (Human)
• Park, KH;Lee, KN;Oh, E;Im, EM; Inflammatory biomarkers in the cervicovaginal fluid to identify histologic chorioamnionitis and funisitis in women with preterm labor Cytokine 2023-09-01 [PMID: 37536223] (Human)
• Henrioux, F;Navel, V;Belville, C;Charnay, C;Antoine, A;Chiambaretta, F;Sapin, V;Blanchon, L; Inflammation of Dry Eye Syndrome: A Cellular Study of the Epithelial and Macrophagic Involvement of NFAT5 and RAGE International journal of molecular sciences 2023-07-04 [PMID: 37446230] (Human)
• A Daily, P Ravishanka, S Harms, VS Klimberg Using tears as a non-invasive source for early detection of breast cancer PLoS ONE, 2022-04-26;17(4):e0267676. 2022-04-26 [PMID: 35471994] (Human)
• LE Cabrera, PT Pekkarinen, M Alander, KHA Nowlan, NA Nguyen, S Jokiranta, S Kuivanen, A Patjas, S Mero, SH Pakkanen, S Heinonen, A Kantele, O Vapalahti, E Kekäläinen, T Strandin Characterization of low-density granulocytes in COVID-19 PloS Pathogens, 2021-07-06;17(7):e1009721. 2021-07-06 [PMID: 34228753] (Human)
• H Xiang, F Guo, X Tao, Q Zhou, S Xia, D Deng, L Li, D Shang Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation Theranostics, 2021-03-04;11(9):4467-4482. 2021-03-04 [PMID: 33754072] (Human)
• M Raundhal, S Ghosh, SA Myers, MS Cuoco, M Singer, SA Carr, SS Waikar, JV Bonventre, J Ritz, RM Stone, DP Steensma, A Regev, LH Glimcher Blockade of IL-22 signaling reverses erythroid dysfunction in stress-induced anemias Nature Immunology, 2021-03-22;0(0):. 2021-03-22 [PMID: 33753942] (Human)
• JS Ha, HR Choi, IS Kim, EA Kim, SW Cho, SJ Yang Hypoxia-Induced S100A8 Expression Activates Microglial Inflammation and Promotes Neuronal Apoptosis International Journal of Molecular Sciences, 2021-01-26;22(3):. 2021-01-26 [PMID: 33530496] (Human)
• NB Leimkühler, HFE Gleitz, L Ronghui, IAM Snoeren, SNR Fuchs, JS Nagai, B Banjanin, KH Lam, T Vogl, C Kuppe, USA Stalmann, G Büsche, H Kreipe, I Gütgemann, P Krebs, Y Banz, P Boor, EW Tai, TH Brümmendor, S Koschmiede, M Crysandt, E Bindels, R Kramann, IG Costa, RK Schneider Heterogeneous bone-marrow stromal progenitors drive myelofibrosis via a druggable alarmin axis Cell Stem Cell, 2020-12-09;0(0):. 2020-12-09 [PMID: 33301706] (Human)
• J Ho, DN Wickramasi, SA Nikou, B Hube, JP Richardson, JR Naglik Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells Cells, 2020-03-12;9(3):. 2020-03-12 [PMID: 32178483] (Human)
• MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads PLoS ONE, 2018-01-25;13(1):e0191676. 2018-01-25 [PMID: 29370247] (Human)
• A transcriptome-based global map of signaling pathways in the ovarian cancer microenvironment associated with clinical outcome Genome Biol, 2016-05-23;17(1):108. 2016-05-23 [PMID: 27215396] (Human)

FAQs for S100A8 (DY4570-05). (Showing 1 - 3 of 3 FAQs).

1. Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
   • Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
   • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.
3. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: S100A8