Human Pappalysin-1/PAPP-A Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Pappalysin-1/PAPP-A Quantikine ELISA Kit Summary

• Background: The Quantikine Human PAPP-A Immunoassay is a 5.5 hour sandwich-type solid phase ELISA designed to measure human PAPP-A in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human PAPP-A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PAPP-A showed linear curves that were parallel to the standard curves ob...tained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring PAPP-A.
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• Specificity: Natural and recombinant human PAPP-A
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: PAPPA

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Pappalysin-1/PAPP-A Quantikine ELISA Kit

• ASBABP2
• DIPLA1
• EC 3.4.24.79
• IGFBP-4ase
• IGFBP-4aseaspecific BCL2 ARE-binding protein 2
• IGF-dependent IGFBP-4 protease
• insulin-like growth factor-dependent IGF binding protein-4 protease
• Insulin-like growth factor-dependent IGF-binding protein 4 protease
• PAPA
• PAPPA
• PAPPA1
• PAPP-A1
• PAPP-Adifferentially placenta 1 expressed protein
• Pappalysin1
• Pappalysin-1
• pregnacy-associated plasma protein A
• Pregnancy-associated plasma protein A
• pregnancy-associated plasma protein A, pappalysin 1

Background

Pappalysins comprise one of the five families of Metzincins (other families include ADAMs/ADAMTSs, MMPs, Astacins, and Serralysins). Pappalysin-1 (PAPP-A) is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy. Reduced maternal serum PAPP-A is a major marker of Down syndrome in the first trimester of pregnancy. PAPP-A cleaves IGFBP-4 and -5 (insulin-like growth factor-binding protein-4 and 5) at a single site, resulting in the release of bioactive IGF. Pappalysin-2 (PAPP-A2; also called PAPP-E) shares 45% amino acid identity with PAPP-A and cleaves IGFBP-2.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Pappalysin-1/PAPP-A (DPPA00)(6)

Publications using DPPA00

• M Babi? Leko, M Mihel?i?, J Jurasovi?, M Nikolac Pe, E Špani?, A Sekovani?, T Orct, K Zub?i?, L Langer Hor, N Plei?, S Ki?emet-Pi, Ž Vogrinc, N Pivac, A Diana, F Borove?ki, PR Hof, G Šimi? Heavy Metals and Essential Metals Are Associated with Cerebrospinal Fluid Biomarkers of Alzheimer's Disease International Journal of Molecular Sciences, 2022-12-27;24(1):. 2022-12-27 [PMID: 36613911] (Human)
• E Slocum, A Craig, A Villanueva, D Germain Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2 Breast Cancer Res., 2019-05-02;21(1):56. 2019-05-02 [PMID: 31046834] (Human)
• S Senapati, MD Sammel, SF Butts, P Takacs, K Chung, KT Barnhart Predicting first trimester pregnancy outcome: derivation of a multiple marker test Fertil. Steril., 2016-10-26;106(7):1725-1732.e3. 2016-10-26 [PMID: 28340932] (Human)
• Yukie Takabatake, Claus Oxvig, Chandandeep Nagi, Kerin Adelson, Shabnam Jaffer, Hank Schmidt et al. Lactation opposes pappalysin‐1‐driven pregnancy‐associated breast cancer EMBO Molecular Medicine 2016-04-01 [PMID: 26951623] (Human)
• Blankley RT, Gaskell SJ, Whetton AD A proof-of-principle gel-free proteomics strategy for the identification of predictive biomarkers for the onset of pre-eclampsia. BJOG, 2009-08-04;116(11):1473-80. 2009-08-04 [PMID: 19663911] (Human)
• Nishizawa H, Pryor-Koishi K, Suzuki M Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients. Mol. Hum. Reprod., 2008-09-18;14(10):595-602. 2008-09-18 [PMID: 18805800] (Human)

FAQs for Pappalysin-1/PAPP-A (DPPA00). (Showing 1 - 2 of 2 FAQs).

1. I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).
   • Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PAPPA