Human Osteopontin (OPN) DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Osteopontin (OPN) DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: SPP1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Osteopontin (OPN) DuoSet ELISA, 15 Plate

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

Publications for Osteopontin/OPN (DY1433)(31)

Publications using DY1433

• Torino, C;Carbone, F;Pizzini, P;Mezzatesta, S;D'Arrigo, G;Gori, M;Liberale, L;Moriero, M;Michelauz, C;Frè, F;Isoppo, S;Gavoci, A;Rosa, F;Scuricini, A;Tirandi, A;Ramoni, D;Mallamaci, F;Tripepi, G;Montecucco, F;Zoccali, C; Osteopontin and Clinical Outcomes in Hemodialysis Patients Biomedicines 2024-11-14 [PMID: 39595171] (Human)
• Kakucs, Z;Illes, Z;Hayden, Z;Berki, T;Orsi, G; Osteopontin predicts late-time salience network-related functional connectivity in multiple sclerosis PloS one 2024-08-29 [PMID: 39208261] (Human)
• Pappas, AG;Eleftheriou, K;Vlahakos, V;Magkouta, SF;Riba, T;Dede, K;Siampani, R;Kompogiorgas, S;Polydora, E;Papalampidou, A;Loutsidi, NE;Mantas, N;Tavernaraki, E;Exarchos, D;Kalomenidis, I; High Plasma Osteopontin Levels Are Associated with Serious Post-Acute-COVID-19-Related Dyspnea Journal of clinical medicine 2024-01-10 [PMID: 38256526] (Human)
• Rehage, E;Sowislok, A;Busch, A;Papaeleftheriou, E;Jansen, M;Jäger, M; Surgical Site-Released Tissue Is Potent to Generate Bone onto TCP and PCL-TCP Scaffolds In Vitro International journal of molecular sciences 2023-11-01 [PMID: 37958857] (Human)
• Matasariu, DR;Bausic, AIG;Mandici, CE;Bujor, IE;Cristofor, AE;Bratila, E;Lozneanu, L;Boiculese, LV;Grigore, M;Ursache, A; Effects of Progestin on Modulation of the Expression of Biomarkers in Endometriosis Biomedicines 2023-07-20 [PMID: 37509675] (Human)
• FZ Khamissi, L Ning, E Kefaloyian, H Dun, A Arthanaris, A Keller, JJ Atkinson, W Li, B Wong, S Dietmann, K Lavine, D Kreisel, A Herrlich Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure Science Advances, 2022-02-25;8(8):eabm5900. 2022-02-25 [PMID: 35213222] (Human)
• R Pohl, S Feder, EM Haberl, L Rein-Fisch, TS Weiss, M Spirk, A Bruckmann, N McMullen, CJ Sinal, C Buechler Chemerin Overexpression in the Liver Protects against Inflammation in Experimental Non-Alcoholic Steatohepatitis Biomedicines, 2022-01-07;10(1):. 2022-01-07 [PMID: 35052810] (Human)
• G Orsi, Z Hayden, T Cseh, T Berki, Z Illes Osteopontin levels are associated with late-time lower regional brain volumes in multiple sclerosis Scientific Reports, 2021-12-08;11(1):23604. 2021-12-08 [PMID: 34880402] (Human)
• LA Henze, M Estepa, B Pieske, F Lang, KU Eckardt, I Alesutan, J Voelkl Zinc Ameliorates the Osteogenic Effects of High Glucose in Vascular Smooth Muscle Cells Cells, 2021-11-09;10(11):. 2021-11-09 [PMID: 34831306] (Human)
• A Larsson, J Helmersson, L Lind, J Ärnlöv, TR Feldreich Strong Associations between Plasma Osteopontin and Several Inflammatory Chemokines, Cytokines, and Growth Factors Biomedicines, 2021-07-28;9(8):. 2021-07-28 [PMID: 34440113] (Human)
• R Urbanavi?i, R Zabitait?, A Kriš?iukai, VP Deltuva, D Skiriut? Serum protein triplet TGF-&beta1, TIMP-1, and YKL-40 serve as diagnostic and prognostic profile for astrocytoma Scientific Reports, 2021-06-23;11(1):13100. 2021-06-23 [PMID: 34162919] (Human)
• G Bai, D Furushima, T Niki, T Matsuba, Y Maeda, A Takahashi, T Hattori, Y Ashino High Levels of the Cleaved Form of Galectin-9 and Osteopontin in the Plasma Are Associated with Inflammatory Markers That Reflect the Severity of COVID-19 Pneumonia International Journal of Molecular Sciences, 2021-05-07;22(9):. 2021-05-07 [PMID: 34067072] (Human)
• Z Macek Jílk, A Seigneurin, C Coppard, L Ouaguia, C Aspord, PN Marche, V Leroy, T Decaens Circulating IL-13 Is Associated with De Novo Development of HCC in HCV-Infected Patients Responding to Direct-Acting Antivirals Cancers, 2020-12-18;12(12):. 2020-12-18 [PMID: 33352852] (Human)
• A Marisetty, J Wei, LY Kong, M Ott, D Fang, A Sabbagh, AB Heimberger MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme Cancers, 2020-12-17;12(12):. 2020-12-17 [PMID: 33348707] (Human)
• G Bai, T Matsuba, T Niki, T Hattori Stimulation of THP-1 Macrophages with LPS Increased the Production of Osteopontin-Encapsulating Exosome Int J Mol Sci, 2020-11-11;21(22):. 2020-11-11 [PMID: 33187327] (Human)
• ST Padilla, T Niki, D Furushima, G Bai, H Chagan-Yas, EF Telan, RJ Tactacan-A, Y Maeda, R Solante, T Hattori Plasma Levels of a Cleaved Form of Galectin-9 Are the Most Sensitive Biomarkers of Acquired Immune Deficiency Syndrome and Tuberculosis Coinfection Biomolecules, 2020-10-30;10(11):. 2020-10-30 [PMID: 33143141] (Human)
• N Sadaghianl, J Contenti, M Dufies, J Parola, M Rouleau, S Lee, JF Peyron, L Fabbri, R Hassen-Kho, J Pouysségur, F Bost, E Jean-Bapti, A Dardik, NM Mazure Co-culture of human fibroblasts, smooth muscle and endothelial cells promotes osteopontin induction in hypoxia J. Cell. Mol. Med., 2020-02-07;0(0):. 2020-02-07 [PMID: 32032472] (Human)
• MN Ali, M Mori, TCJ Mertens, P Siddhuraj, JS Erjefält, P Önnerfjord, PS Hiemstra, A Egesten Osteopontin Expression in Small Airway Epithelium in Copd is Dependent on Differentiation and Confined to Subsets of Cells Sci Rep, 2019-10-29;9(1):15566. 2019-10-29 [PMID: 31664154] (Human)
• MG Abate, L Moretto, I Licari, T Esposito, L Capuano, C Olivieri, A Benech, M Brucoli, GC Avanzi, G Cammarota, U Dianzani, N Clemente, G Panzarasa, G rio, F Carfagna, G Cappellano, F Della Cort, R Vaschetto Osteopontin in the Cerebrospinal Fluid of Patients with Severe Aneurysmal Subarachnoid Hemorrhage Cells, 2019-07-10;8(7):. 2019-07-10 [PMID: 31295895] (Human)
• KJ Lenos, DM Miedema, SC Lodestijn, LE Nijman, T van den Bo, X Romero Ros, FC Lourenço, MC Lecca, M van der He, SM van Neerve, A van Oort, N Leveille, RS Adam, F de Sousa E, J Otten, P Veerman, G Hypolite, L Koens, SK Lyons, G Stassi, DJ Winton, JP Medema, E Morrissey, MF Bijlsma, L Vermeulen Stem cell functionality is microenvironmentally defined during tumour expansion and therapy response in colon cancer Nat. Cell Biol., 2018-09-03;0(0):. 2018-09-03 [PMID: 30177776] (Xenograft)
• J Panezai, A Ghaffar, M Altamash, PE Engström, A Larsson Periodontal disease influences osteoclastogenic bone markers in subjects with and without rheumatoid arthritis PLoS ONE, 2018-06-11;13(6):e0197235. 2018-06-11 [PMID: 29889832] (Human)
• G Bai, H Motoda, R Ozuru, H Chagan-Yas, T Hattori, T Matsuba Synthesis of a Cleaved Form of Osteopontin by THP-1 Cells and Its Alteration by Phorbol 12-Myristate 13-Acetate and BCG Infection Int J Mol Sci, 2018-01-31;19(2):. 2018-01-31 [PMID: 29385060] (Human)
• P Lochner, R Cantello, K Fassbender, M Lesmeister, R Nardone, A Siniscalch, N Clemente, A Naldi, L Coppo, F Brigo, C Comi Longitudinal Assessment of Transorbital Sonography, Visual Acuity, and Biomarkers for Inflammation and Axonal Injury in Optic Neuritis Dis. Markers, 2017-09-11;2017(0):5434310. 2017-09-11 [PMID: 29085182] (Human)
• J Zhao, B Shiratori, H Chagan-Yas, M Matsumoto, T Niki, M Tanaka, Y Takahashi, O Usami, Y Ashino, T Hattori Secretion of IFN-? Associated with Galectin-9 Production by Pleural Fluid Cells from a Patient with Extrapulmonary Tuberculosis Int J Mol Sci, 2017-06-28;18(7):. 2017-06-28 [PMID: 28657598] (Human)
• H Lavi, M Assayag, A Schwartz, N Arish, ZG Fridlender, N Berkman The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis PLoS ONE, 2017-03-02;12(3):e0171945. 2017-03-02 [PMID: 28253271] (Human)
• Q Ge, CC Ruan, Y Ma, XF Tang, QH Wu, JG Wang, DL Zhu, PJ Gao Osteopontin regulates macrophage activation and osteoclast formation in hypertensive patients with vascular calcification Sci Rep, 2017-01-16;7(0):40253. 2017-01-16 [PMID: 28091516] (Human)
• Gordin D, Forsblom C, Panduru N, Thomas M, Bjerre M, Soro-Paavonen A, Tolonen N, Sandholm N, Flyvbjerg A, Harjutsalo V, Groop P Osteopontin is a strong predictor of incipient diabetic nephropathy, cardiovascular disease, and all-cause mortality in patients with type 1 diabetes. Diabetes Care, 2014-06-26;37(9):2593-600. 2014-06-26 [PMID: 24969575] (Human)
• Wulf-Johansson H, Lock Johansson S, Schlosser A, Trommelholt Holm A, Rasmussen L, Mickley H, Diederichsen A, Munkholm H, Poulsen T, Tornoe I, Nielsen V, Marcussen N, Vestbo J, Saekmose S, Holmskov U, Sorensen G Localization of microfibrillar-associated protein 4 (MFAP4) in human tissues: clinical evaluation of serum MFAP4 and its association with various cardiovascular conditions. PLoS ONE, 2013-12-13;8(12):e82243. 2013-12-13 [PMID: 24349233] (Human)
• Chu M, Yang P, Hu R, Hou S, Li F, Chen Y, Kijlstra A Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease. Invest. Ophthalmol. Vis. Sci., 2011-09-09;52(10):7084-9. 2011-09-09 [PMID: 21810982] (Human)
• Samitas K, Zervas E, Vittorakis S, Semitekolou M, Alissafi T, Bossios A, Gogos H, Economidou E, Lotvall J, Xanthou G, Panoutsakopoulou V, Gaga M Osteopontin expression and relation to disease severity in human asthma. Eur. Respir. J., 2010-06-18;37(2):331-41. 2010-06-18 [PMID: 20562127] (Human)
• Wu IC, Wu DC, Huang CC Plasma decorin predicts the presence of esophageal squamous cell carcinoma. Int. J. Cancer, 2010-11-01;127(9):2138-46. 2010-11-01 [PMID: 20143390] (Human)

FAQs for Osteopontin/OPN (DY1433). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SPP1