Human Nesfatin-1/Nucleobindin-2 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Nesfatin-1/Nucleobindin-2 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: NUCB2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Nesfatin-1/Nucleobindin-2 DuoSet ELISA, 15 Plate

• DNA-binding protein NEFA
• NEFA
• NEFAGastric cancer antigen Zg4
• Nesfatin1
• Nesfatin-1
• NUCB2
• nucleobindin 2
• Nucleobindin-2
• nucleobinding 2

Background

Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1- 24), Nesfatin-1 (aa 25-106), a DNA-binding site (aa 171-223), and two EF-hand regions (aa 241-276 and 293-328). Intracellular uncleaved NUCB2 is 45-50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109-187) and Nesfatin-3 (aa 190-420). Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.

Publications for Nesfatin-1/Nucleobindin-2 (DY5949)(6)

Publications using DY5949

• Zimowska, M;Rolbiecka, M;Antoniak-Pietrynczak, K;Jaskulak, M;Zorena, K; Dynamics of Serum Inflammatory Markers and Adipokines in Patients: Implications for Monitoring Abnormal Body Weight: Preliminary Research Metabolites 2024-05-01 [PMID: 38786737] (Human)
• KD Honoré, S Bruun, LN Jacobsen, M Domellöf, KF Michaelsen, S Husby, G Zachariass Nesfatin-1 in Human Milk and Its Association with Infant Anthropometry Nutrients, 2022-12-30;15(1):. 2022-12-30 [PMID: 36615833] (Human)
• BM Giglio, RM Schincagli, AS da Silva, ICS Fazani, PA Monteiro, JF Mota, JP Cunha, C Pichard, GD Pimentel Whey Protein Supplementation Compared to Collagen Increases Blood Nesfatin Concentrations and Decreases Android Fat in Overweight Women: A Randomized Double-Blind Study Nutrients, 2019-09-02;11(9):. 2019-09-02 [PMID: 31480676] (Human)
• C Robinson, L Tsang, A Solomon, AJ Woodiwiss, S Gunter, M Mer, HC Hsu, M Gomes, GR Norton, AME Millen, PH Dessein Nesfatin-1 and visfatin expression is associated with reduced atherosclerotic disease risk in patients with rheumatoid arthritis Peptides, 2018-02-21;102(0):31-37. 2018-02-21 [PMID: 29475075] (Human)
• Nesfatin-1/Nucleobindin-2 enhances cell migration, invasion, and epithelial-mesenchymal transition via LKB1/AMPK/TORC1/ZEB1 pathways in colon cancer Oncotarget, 2016-05-24;7(21):31336-49. 2016-05-24 [PMID: 27150059] (Human)
• Dong J, Xu H, Xu H, Wang P, Cai G, Song H, Wang C, Dong Z, Ju Y, Jiang Z Nesfatin-1 stimulates fatty-acid oxidation by activating AMP-activated protein kinase in STZ-induced type 2 diabetic mice. PLoS ONE, 2013-12-31;8(12):e83397. 2013-12-31 [PMID: 24391760] (Mouse)

FAQs for Nesfatin-1/Nucleobindin-2 (DY5949). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NUCB2