Human MFG-E8 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human MFG-E8 Quantikine ELISA Kit Summary

• Background: The Quantikine Human MFG-E8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MFG-E8 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MFG-E8. Results obtained using natural hu...man MFG-E8 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MFG-E8.
  
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• Specificity: Natural and recombinant human MFG-E8.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: MFGE8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human MFG-E8 Quantikine ELISA Kit

• BA46
• Breast epithelial antigen BA46
• EDIL1
• hP47
• HsT19888
• lactadherin
• Lactahedrin
• Medin
• MFG1
• MFGE8
• MFG-E8
• MFGM
• milk fat globule-EGF factor 8 protein
• Milk fat globule-EGF factor 8
• O-acetyl disialoganglioside synthase
• OAcGD3S
• SED1
• SPAG10
• sperm associated antigen 10
• sperm surface protein hP47

Background

MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for MFG-E8 (DFGE80)(15)

Publications using DFGE80

• Muhandiram, S;Kodithuwakku, S;Godakumara, K;Fazeli, A; Rapid increase of MFGE8 secretion from endometrial epithelial cells is an indicator of extracellular vesicle mediated embryo maternal dialogue Scientific reports 2024-10-29 [PMID: 39472639] (Human)
• Urban, L;Novák, Š;?oma, M;Dvo?ánková, B;Lacina, L;Šáchová, J;Hradilová, M;Svato?ová, P;Kolá?, M;Strnad, H;B?ezinová, J;Smetana, K;Gál, P;Szabo, P; Unravelling heterogeneous effects of cancer?associated fibroblasts on poor prognosis markers in breast cancer EM?G3 cell line: In vitro?targeted treatment (anti?IL-6, anti?VEGF-A, anti?MFGE8) based on transcriptomic profiling Oncology reports 2024-01-01 [PMID: 37975220] (Human)
• Muhandiram, S;Dissanayake, K;Orro, T;Godakumara, K;Kodithuwakku, S;Fazeli, A; Secretory Proteomic Responses of Endometrial Epithelial Cells to Trophoblast-Derived Extracellular Vesicles International journal of molecular sciences 2023-07-25 [PMID: 37569298] (Human)
• J Wagner, K Degenhardt, M Veit, N Louros, K Konstantou, A Skodras, K Wild, P Liu, U Obermüller, V Bansal, A Dalmia, LM Häsler, M Lambert, M De Vleesch, HA Davies, J Madine, D Kronenberg, R Feederle, D Del Turco, KPR Nilsson, T Lashley, T Deller, M Gearing, LC Walker, P Heutink, F Rousseau, J Schymkowit, M Jucker, JJ Neher Medin co-aggregates with vascular amyloid-beta in Alzheimer's disease Nature, 2022-11-16;0(0):. 2022-11-16 [PMID: 36385530] (Human)
• J Štšepetova, K Simre, A Tagoma, O Uibo, A Peet, H Siljander, V Tillmann, M Knip, R Mändar, R Uibo Maternal breast milk microbiota and immune markers in relation to subsequent development of celiac disease in offspring Scientific Reports, 2022-04-22;12(1):6607. 2022-04-22 [PMID: 35459889] (Human)
• P Marazuela, M Solé, A Bonaterra-, J Pizarro, J Camacho, E Martínez-S, HB Kuiperij, MM Verbeek, AM de Kort, FHBM Schreuder, CJM Klijn, L Castillo-R, O Pancorbo, D Rodríguez-, F Pujadas, P Delgado, M Hernández- MFG-E8 (LACTADHERIN): a novel marker associated with cerebral amyloid angiopathy Acta neuropathologica communications, 2021-09-16;9(1):154. 2021-09-16 [PMID: 34530925] (Human)
• K Han, K Singh, MJ Rodman, S Hassanzade, Y Baumer, RD Huffstutle, J Chen, J Candia, F Cheung, KER Stagliano, M Pirooznia, TM Powell-Wil, MN Sack Identification and Validation of Nutrient State-Dependent Serum Protein Mediators of Human CD4+ T Cell Responsiveness Nutrients, 2021-04-28;13(5):. 2021-04-28 [PMID: 33924911] (Human)
• JA Asaro, Z Khan, M Brewer, K Klose, C Pesce, RJ Schanler, CN Codipilly Relationship Between Milk Fat Globule-Epidermal Growth Factor 8 and Intestinal Cytokines in Infants Born Preterm J Pediatr, 2020-11-09;0(0):. 2020-11-09 [PMID: 33181195] (Human)
• WY Baek, JM Woo, HA Kim, JY Jung, CH Suh Polymorphisms of MFGE8 are associated with susceptibility and clinical manifestations through gene expression modulation in Koreans with systemic lupus erythematosus Sci Rep, 2019-12-06;9(1):18565. 2019-12-06 [PMID: 31811237] (Human)
• LP Foltz, SE Howden, JA Thomson, DO Clegg Functional Assessment of Patient-Derived Retinal Pigment Epithelial Cells Edited by CRISPR/Cas9 Int J Mol Sci, 2018-12-19;19(12):. 2018-12-19 [PMID: 30572641] (Human)
• C Müller, C Charniga, S Temple, SC Finnemann Quantified F-Actin Morphology Is Predictive of Phagocytic Capacity of Stem Cell-Derived Retinal Pigment Epithelium Stem Cell Reports, 2018-02-15;0(0):. 2018-02-15 [PMID: 29456184] (Human)
• C Kishi, SI Motegi, O Ishikawa Elevated serum MFG-E8 level is possibly associated with the presence of high-intensity cerebral lesions on magnetic resonance imaging in patients with systemic lupus erythematosus J. Dermatol, 2017-03-07;0(0):. 2017-03-07 [PMID: 28266034] (Human)
• Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes J Immunol, 2016-05-18;0(0):. 2016-05-18 [PMID: 27194784] (Human)
• W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter Exosomes in Human Breast Milk Promote EMT Clin Cancer Res, 2016-04-08;0(0):. 2016-04-08 [PMID: 27060153] (Human)
• Uchiyama A, Yamada K, Ogino S, Yokoyama Y, Takeuchi Y, Udey M, Ishikawa O, Motegi S MFG-E8 regulates angiogenesis in cutaneous wound healing. Am J Pathol, 2014-05-15;184(7):1981-90. 2014-05-15 [PMID: 24838098] (Human)

FAQs for MFG-E8 (DFGE80). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: MFGE8