Human Klotho DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Klotho DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: KL

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Klotho DuoSet ELISA, 5 Plate

• EC 3.2.1
• EC 3.2.1.31
• KL
• Klotho

Background

The Klotho proteins, Klotho and Klotho beta (beta KL), are type I transmembrane glycoproteins with extracellular regions that contain two beta-glycosidase-like repeats. Klotho has both membrane-bound and soluble forms; the soluble form predominates and consists of only the first beta-glycosidase-like repeat. Klotho has beta-glucuronidase activity against select steroid beta-glucuronides, appears to regulate calcium and vitamin D levels in serum, and is associated with premature aging.

Klotho beta, a divergent structural member of the glycosidase I superfamily, is primarily expressed in the liver and pancreas, with lower expression in adipose tissue. Klotho beta mediates the interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver, which negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes. Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes. The expression pattern of Klotho beta suggests it may play a role in lipid metabolism.

Publications for Klotho (DY5334-05)(13)

Publications using DY5334-05

• Moura-Maia, MS;Brill, B;Paula-Vieira, RHR;Ramos-Gomes, NV;Melamed, D;Silva-Reis, A;Wolpp, ETR;Moreira-Silva, NN;Bella, YF;Vieira, RP; Low Caloric Intake Confers Cardiovascular Protection and Improves Functional Capacity Without Affecting Immunological Response in Sedentary Older Adults Nutrients 2024-10-29 [PMID: 39519510] (Human)
• Pei, Y;Huang, W;Cao, L;Yang, F;Chi, C;Zhu, J; Serum Klotho Is Elevated in Patients with Acute Myocardial Infarction and Could Predict Poor In-Hospital Prognosis Journal of cardiovascular development and disease 2024-09-20 [PMID: 39330350] (Human)
• Barbu, E;Mihaila, AC;Gan, AM;Ciortan, L;Macarie, RD;Tucureanu, MM;Filippi, A;Stoenescu, AI;Petrea, SV;Simionescu, M;Balanescu, SM;Butoi, E; The Elevated Inflammatory Status of Neutrophils Is Related to In-Hospital Complications in Patients with Acute Coronary Syndrome and Has Important Prognosis Value for Diabetic Patients International journal of molecular sciences 2024-05-08 [PMID: 38791147] (Human)
• Chen, MH;Lin, WC;Li, CT;Wu, HJ;Bai, YM;Tsai, SJ;Su, TP;Tu, PC; Effects of low-dose ketamine infusion on vascular endothelial growth factor and matrix metalloproteinase-9 among patients with treatment-resistant depression and suicidal ideation Journal of psychiatric research 2023-07-19 [PMID: 37487293] (Human)
• D Aczel, F Torma, M Jokai, K McGreevy, A Boros, Y Seki, I Boldogh, S Horvath, Z Radak The Circulating Level of Klotho Is Not Dependent upon Physical Fitness and Age-Associated Methylation Increases at the Promoter Region of the Klotho Gene Genes, 2023-02-19;14(2):. 2023-02-19 [PMID: 36833453] (Human)
• Y Lin, J Huang, M Zhou, C Pan, A Shan, C Zhou Association of serum klotho levels with different-staged vascular calcification status in patients with maintenance hemodialysis Bmc Nephrology, 2022-11-19;23(1):374. 2022-11-19 [PMID: 36402949] (Human)
• A Silva-Reis, MA Rodrigues, R Moraes-Fer, TG Gonçalves-, VH Souza-Palm, HC Aquino-San, ALL Bachi, LVF de Oliveir, RÁB Lopes-Mart, I Oliveira-S, R Albertini, CR Frison, RP Vieira Combined resistance and aerobic training improves lung function and mechanics and fibrotic biomarkers in overweight and obese women Frontiers in Physiology, 2022-09-07;13(0):946402. 2022-09-07 [PMID: 36160852] (Human)
• M Yeganeh-Ha, H Najafipour, F Rostamzade, A Naghibzade Klotho and SIRT1 changes from pre-diabetes to diabetes and pre-hypertension to hypertension Diabetology & metabolic syndrome, 2021-10-20;13(1):115. 2021-10-20 [PMID: 34670596] (Human)
• MAR Brandao-Ra, R Moraes-Fer, MC Oliveira-J, A Santos-Dia, ALL Bachi, G Gabriela-P, S de Oliveir, AC Araújo-Ros, LVF Oliveira, CR Frison, WL do Prado, RP Raju, PB Balagopal, RP Vieira Pulmonary function changes in older adults with and without metabolic syndrome Scientific Reports, 2021-08-30;11(1):17337. 2021-08-30 [PMID: 34462482] (Human)
• WY Liang, LH Wang, JH Wei, QL Li, QY Li, Q Liang, NQ Hu, LH Li No significant association of serum klotho concentration with blood pressure and pulse wave velocity in a Chinese population Scientific Reports, 2021-01-27;11(1):2374. 2021-01-27 [PMID: 33504927] (Human)
• A Farías-Bas, HR Martínez-R, EF Gómez-Garc, AM Cueto-Manz, L Cortés-San, LE Hernández-, G Ramírez-Ló, F Mendoza-Ca Circulating Levels of Soluble Klotho and Fibroblast Growth Factor 23 in Mexican Diabetic Patients and Its Association With Early Nephropathy Arch. Med. Res., 2019-02-01;0(0):. 2019-02-01 [PMID: 30718148] (Human)
• A Chen, Y Sun, J Cui, B Zhao, H Wang, X Chen, Y Mao Associations of sclerostin with carotid artery atherosclerosis and all-cause mortality in Chinese patients undergoing maintenance hemodialysis BMC Nephrol, 2018-10-12;19(1):264. 2018-10-12 [PMID: 30314461] (Human)
• Almroth G, Lonn J, Uhlin F, Brudin L, Andersson B, Hahn-Zoric M Sclerostin, TNF-alpha and Interleukin-18 Correlate and are Together with Klotho Related to Other Growth Factors and Cytokines in Haemodialysis Patients. Scand J Immunol, 2016-01-01;83(1):58-63. 2016-01-01 [PMID: 26448366] (Human)

FAQs for Klotho (DY5334-05). (Showing 1 - 2 of 2 FAQs).

1. I study physiology (PhD) and I need a klotho kit.
   • A list of our klotho products can be found here. I am not entirely sure what you are looking for as I don't have your complete message for some reason. Can you please clarify what you are looking for?
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: KL