Human IL-32 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-32 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL32

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-32 DuoSet ELISA, 5 Plate

• IL32
• IL-32
• IL-32alpha
• IL-32beta
• interleukin 32
• interleukin-32 theta
• interleukin-32
• natural killer cell transcript 4
• Natural killer cells protein 4
• NK4
• NK4IL-32delta
• TAIF
• TAIFa
• TAIFb
• TAIFc
• TAIFd
• TAIFIL-32gamma
• Tumor necrosis factor alpha-inducing factor

Background

IL-32 alpha is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32 beta, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN-gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn induces cytokine expression. No ortholog has been found in mouse.

Publications for IL-32 (DY3040-05)(10)

Publications using DY3040-05

• Mester, P;Utrata, A;Schmidtner, N;Birner, C;Schmid, S;Müller, M;Pavel, V;Buechler, C; Lower Plasma IL-32 Levels Linked to Better Survival in Sepsis Biomedicines 2025-03-19 [PMID: 40149726] (Human)
• Soussi, S;Maione, AS;Lefèvre, L;Pizzinat, N;Iacovoni, J;Gonzalez-Fuentes, I;Cussac, D;Iengo, L;Santin, Y;Tundo, F;Tondo, C;Pompilio, G;Parini, A;Douin-Echinard, V;Sommariva, E; Analysis of effector/memory regulatory T cells from arrhythmogenic cardiomyopathy patients identified IL-32 as a novel player in ACM pathogenesis Cell death & disease 2025-02-11 [PMID: 39934117] (Human)
• M Tomasi, A Cherubini, S Pelusi, S Margarita, C Bianco, F Malvestiti, L Miano, S Romeo, D Prati, L Valenti Circulating Interlukin-32 and Altered Blood Pressure Control in Individuals with Metabolic Dysfunction International Journal of Molecular Sciences, 2023-04-18;24(8):. 2023-04-18 [PMID: 37108628] (Human)
• H Chen, W Yang, X Xue, Y Li, Z Jin, Z Ji Integrated Analysis Revealed an Inflammatory Cancer-Associated Fibroblast-Based Subtypes with Promising Implications in Predicting the Prognosis and Immunotherapeutic Response of Bladder Cancer Patients International Journal of Molecular Sciences, 2022-12-15;23(24):. 2022-12-15 [PMID: 36555612] (Human)
• YLL Braga, JRC Neto, AWF Costa, MVT Silva, MV Silva, MRN Celes, MAP Oliveira, LAB Joosten, F Ribeiro-Di, RS Gomes, JR Machado Interleukin-32gamma in the Control of Acute Experimental Chagas Disease Journal of Immunology Research, 2022-01-20;2022(0):7070301. 2022-01-20 [PMID: 35097133] (Mouse)
• JC Dos Santos, AM Barroso de, MV Teodoro Si, B Cirovic, LCJ de Bree, MSMA Damen, SJCFM Moorlag, RS Gomes, MM Helsen, M Oosting, ST Keating, A Schlitzer, MG Netea, F Ribeiro-Di, LAB Joosten β-Glucan-Induced Trained Immunity Protects against Leishmania braziliensis Infection: a Crucial Role for IL-32 Cell Rep, 2019-09-03;28(10):2659-2672.e6. 2019-09-03 [PMID: 31484076] (Human, Transgenic Mouse)
• R Fadaei, N Bagheri, E Heidarian, A Nouri, Z Hesari, N Moradi, A Ahmadi, R Ahmadi Serum levels of IL-32 in patients with type 2 diabetes mellitus and its relationship with TNF-α and IL-6 Cytokine, 2019-08-31;125(0):154832. 2019-08-31 [PMID: 31479874] (Human)
• P Comella-De, R Abellana, R Villar-Her, MD Jean Coute, B Sallés Min, L Canales Al, M Narcisse, J Gautier, C Ascaso, I Latorre, J Dominguez, TM Perez-Porc A Model Based on the Combination of IFN-gamma, IP-10, Ferritin and 25-Hydroxyvitamin D for Discriminating Latent From Active Tuberculosis in Children Front Microbiol, 2019-08-14;10(0):1855. 2019-08-14 [PMID: 31474956] (Human)
• Muhammad Zahoor, Marita Westhrin, Kristin Roseth Aass, Siv Helen Moen, Kristine Misund, Katarzyna Maria Psonka-Antonczyk et al. Hypoxia promotes IL-32 expression in myeloma cells, and high expression is associated with poor survival and bone loss Blood Advances 2017-12-26 [PMID: 29296919] (Human)
• H Sato, S Muraoka, N Kusunoki, S Masuoka, S Yamada, H Ogasawara, T Imai, Y Akasaka, N Tochigi, H Takahashi, K Tsuchiya, S Kawai, T Nanki Resistin upregulates chemokine production by fibroblast-like synoviocytes from patients with rheumatoid arthritis Arthritis Res. Ther., 2017-12-01;19(1):263. 2017-12-01 [PMID: 29191223] (Human)

FAQs for IL-32 (DY3040-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL32