Human IL-21 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-21 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-21 DuoSet ELISA, 5 Plate

• CVID11
• IL21
• IL-21
• IL-21Za11interleukin-21
• interleukin 21
• interleukin-21 isoform
• Za11

Background

IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.

Publications for IL-21 (DY8879-05)(12)

Publications using DY8879-05

• Zhu, X;Yu, Y;Li, Y;Wang, P;Li, Y;McCabe, C;Chen, S;Langenfeld, HE;Hanson, AC;Sharp, BE;Woltzen, A;Crowson, CS;Markovic, SN;Davis, JM;Dong, H;Thanarajasingam, U;Zeng, H; Inflammatory arthritis immune related adverse events represent a unique autoimmune disease entity primarily driven by T cells, but likely not autoantibodies medRxiv : the preprint server for health sciences 2025-06-06 [PMID: 40502578] (Human)
• Courtemanche, O;Huppé, CA;Blais-Lecours, P;Maranda, C;Morissette, MC;Blanchet, MR;Dion, G;Marsolais, D; Ex Vivo Overactivation of Lymphocyte Subsets in Fibrotic Hypersensitivity Pneumonitis Is Blunted by a Sphingosine-1-Phosphate Receptor Ligand International journal of molecular sciences 2025-03-29 [PMID: 40243992] (Human)
• Chen, S;Nguyen, TD;Lee, KZ;Liu, D; Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies Biotechnology advances 2024-08-19 [PMID: 39168355] (Human)
• Alipour, S;Amanallahi, P;Baradaran, B;Aghebati-Maleki, A;Soltani-Zangbar, MS;Aghebati-Maleki, L; Altered gene expression of miR-155 in peripheral blood mononuclear cells of Multiple sclerosis patients: Correlation with TH17 frequency, inflammatory cytokine profile and autoimmunity Multiple sclerosis and related disorders 2024-07-07 [PMID: 39033590] (Human)
• Cai, Y;Ji, H;Zhou, X;Zhao, K;Zhang, X;Pan, L;Shi, R; Interleukin-21 modulates balance between regulatory T cells and T-helper 17 cells in chronic hepatitis B virus infection BMC infectious diseases 2023-10-24 [PMID: 37875903] (Human)
• H Asashima, S Mohanty, M Comi, WE Ruff, KB Hoehn, P Wong, J Klein, C Lucas, I Cohen, S Coffey, N Lele, L Greta, K Raddassi, O Chaudhary, A Unterman, B Emu, SH Kleinstein, RR Montgomery, A Iwasaki, CS Dela Cruz, N Kaminski, AC Shaw, DA Hafler, TS Sumida PD-1highCXCR5-CD4+ peripheral helper T�cells promote CXCR3+ plasmablasts in human acute viral infection Cell Reports, 2023-01-02;0(0):111895. 2023-01-02 [PMID: 36596303] (Human)
• H Jiang, CW Wang, Z Wang, Y Dai, Y Zhu, YS Lee, Y Cao, WH Chung, S Ouyang, H Wang Functional and structural characteristics of HLA-B*13:01-mediated specific T cells reaction in dapsone-induced drug hypersensitivity Journal of Biomedical Science, 2022-08-13;29(1):58. 2022-08-13 [PMID: 35964029] (Human)
• A Martner, H Grauers Wi, A Törnell, J Ringlander, M Arabpour, M Lindh, M Lagging, S Nilsson, K Hellstrand Transient and durable T cell reactivity after COVID-19 Oncogene, 2022-07-12;119(30):e2203659119. 2022-07-12 [PMID: 35858456] (Human)
• A Rajamanick, N Pavan Kuma, AN Pandiaraj, N Selvaraj, S Munisankar, RM Renji, V Venkataram, M Murhekar, JWV Thangaraj, SK Muthusamy, GK Chethrapil, T Bhatnagar, M Ponnaiah, S Ramasamy, S Velusamy, S Babu Characterization of memory T cell subsets and common gamma-chain cytokines in convalescent COVID-19 individuals Journal of leukocyte biology, 2022-03-08;0(0):. 2022-03-08 [PMID: 35258122] (Human)
• M Paccagnell, A Abbona, A Michelotti, E Geuna, F Ruatta, E Landucci, N Denaro, P Vanella, C Lo Nigro, D Galizia, M Merlano, O Garrone Circulating Cytokines in Metastatic Breast Cancer Patients Select Different Prognostic Groups and Patients Who Might Benefit from Treatment beyond Progression Vaccines, 2022-01-05;10(1):. 2022-01-05 [PMID: 35062739] (Human)
• Y Hao, L Xie, J Xia, Z Liu, B Yang, M Zhang Plasma interleukin-21 levels and genetic variants are associated with susceptibility to rheumatoid arthritis Bmc Musculoskeletal Disorders, 2021-03-05;22(1):246. 2021-03-05 [PMID: 33673829] (Human)
• W Xu, M Ren, S Ghosh, K Qian, Z Luo, A Zhang, C Zhang, J Cui Defects of CTLA-4 Are Associated with Regulatory T Cells in Myasthenia Gravis Implicated by Intravenous Immunoglobulin Therapy Mediators Inflamm., 2020-02-14;2020(0):3645157. 2020-02-14 [PMID: 32148437] (Human)

FAQs for IL-21 (DY8879-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.