Human IL-15 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

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Catalog# & Conjugate Size Price

Human IL-15 Quantikine ELISA Kit Summary

Background
The Quantikine Human IL-15 Immunoassay is a 4.25 or 4.5 hour solid phase ELISA designed to measure human IL-15 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-15 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human I...L-15 expressed by HuT-102 cells showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-15.
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Specificity
Natural and recombinant human IL-15
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL15

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using D1500.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-15 Quantikine ELISA Kit

  • IL15
  • IL-15
  • IL-15MGC9721
  • interleukin 15
  • interleukin-15

Background

IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-15 (D1500)(63)

We have publications tested in 5 confirmed species: Human, Mouse, Humanized Mouse, Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Primate - Pan troglodytes (Chimpanzee).


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Human
(59)
Mouse
(1)
Humanized Mouse
(1)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
(1)
Primate - Pan troglodytes (Chimpanzee)
(1)
All Species
Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using D1500 Applications Species
Dolna-Michno, J;Kopi?ski, P;Przybylski, G;Wypasek, E;Szyma?ska, M;W?drowska, E;Miko?ajczyk, K;Senderek, T;Gagat, M; Cytoimmunological Profile of Lower Airways in Post-COVID-19 Syndrome (PCS): Predictive Value of Bronchoalveolar Lavage Journal of clinical medicine 2025-05-12 [PMID: 40429360] (Human) Human
Li, YR;Zhou, K;Lee, D;Zhu, Y;Halladay, T;Yu, J;Zhou, Y;Lyu, Z;Fang, Y;Chen, Y;Semaan, S;Yang, L; Generating allogeneic CAR-NKT cells for off-the-shelf cancer immunotherapy with genetically engineered HSP cells and feeder-free differentiation culture Nature protocols 2025-01-17 [PMID: 39825143] (Human) Human
Chen, S;Nguyen, TD;Lee, KZ;Liu, D; Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies Biotechnology advances 2024-08-19 [PMID: 39168355] (Human) Human
Szyma?ska, B;Knysz, B;Ciep?ucha, H;Piwowar, A; Assessment of Metabolic, Inflammatory, and Immunological Disorders Using a New Panel of Plasma Parameters in People Living with HIV Undergoing Antiretroviral Therapy-A Retrospective Study Journal of clinical medicine 2024-08-05 [PMID: 39124846] (Human) Human
Wang, Q;Wei, R;Guo, S;Min, C;Zhong, X;Huang, H;Cheng, Z; An alternative fully human anti-BCMA CAR-T shows response for relapsed or refractory multiple myeloma with anti-BCMA CAR-T exposures previously Cancer gene therapy 2023-12-15 [PMID: 38102463] (Human) Human
Lee, D;Dunn, ZS;Guo, W;Rosenthal, CJ;Penn, NE;Yu, Y;Zhou, K;Li, Z;Ma, F;Li, M;Song, TC;Cen, X;Li, YR;Zhou, JJ;Pellegrini, M;Wang, P;Yang, L; Unlocking the potential of allogeneic V?2 T cells for ovarian cancer therapy through CD16 biomarker selection and CAR/IL-15 engineering Nature communications 2023-11-08 [PMID: 37938576] (Human) Human
Ando, C;Ichihara, E;Nishi, T;Morita, A;Hara, N;Takada, K;Nakasuka, T;Watanabe, H;Kano, H;Nishii, K;Makimoto, G;Kondo, T;Ninomiya, K;Fujii, M;Kubo, T;Ohashi, K;Matsuoka, KI;Hotta, K;Tabata, M;Maeda, Y;Kiura, K; Efficacy of gilteritinib in comparison with alectinib for the treatment of ALK-rearranged non-small cell lung cancer Cancer science 2023-09-15 [PMID: 37715310] (Human) Human
Nosik, M;Belikova, MG;Ryzhov, K;Avdoshina, D;Sobkin, A;Zverev, V;Svitich, O; Unique Profile of Proinflammatory Cytokines in Plasma of Drug-Naïve Individuals with Advanced HIV/TB Co-Infection Viruses 2023-06-06 [PMID: 37376629] (Human) Human
A Bade?ski, M Bade?ska, E ?wi?tochow, A Janek, A Gliwi?ska, A Morawiec-K, M Szczepa?sk Assessment of Interleukin-15 (IL-15) Concentration in Children with Idiopathic Nephrotic Syndrome International Journal of Molecular Sciences, 2023-04-10;24(8):. 2023-04-10 [PMID: 37108157] (Human) Human
SA Abeynaike, TR Huynh, A Mehmood, T Kim, K Frank, K Gao, C Zalfa, A Gandarilla, L Shultz, S Paust Human Hematopoietic Stem Cell Engrafted IL-15 Transgenic NSG Mice Support Robust NK Cell Responses and Sustained HIV-1 Infection Viruses, 2023-01-27;15(2):. 2023-01-27 [PMID: 36851579] (Humanized Mouse) Humanized Mouse
Show All 63 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-15 (D1500). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

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Bioinformatics

Gene Symbol IL15