Human IL-15 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-15 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL15

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-15 DuoSet ELISA, 15 Plate

• IL15
• IL-15
• IL-15MGC9721
• interleukin 15
• interleukin-15

Background

IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.

Publications for IL-15 (DY247)(21)

Publications using DY247

• Yang, J;Xu, B;Cao, J;Liu, Y;Tang, L;Zhao, P;Li, S;Li, X;Liu, J;Yu, R;Tang, Y;Tan, W;Ding, H;Li, J;Liu, Y; Characterizing the mechanisms underpinning interleukin-15R?-mediated protection against sepsis and candidiasis Cytokine 2025-09-17 [PMID: 40967153] (Human)
• Nejatfard, A;Klich, JH;Eckman, N;Bailey, SJ;Gillard, K;Mejia, DR;Ou, BS;Yan, J;Hickey, JW;Appel, EA; A Transient Immunostimulatory Niche Synergizes Adoptive and Endogenous Immunity for Enhanced Tumor Control bioRxiv : the preprint server for biology 2025-08-25 [PMID: 40909524] (Human)
• Fiorillo, A;Gallego, JJ;Casanova-Ferrer, F;Giménez-Garzó, C;Urios, A;Ballester, MP;Durbán, L;Rios, MP;Megías, J;San Miguel, T;Kosenko, E;Escudero-García, D;Benlloch, S;Felipo, V;Montoliu, C; Mild Cognitive Impairment Is Associated with Enhanced Activation of Th17 Lymphocytes in Non-Alcoholic Fatty Liver Disease International journal of molecular sciences 2023-06-20 [PMID: 37373554] (Human)
• P Bajgain, AG Torres Cha, K Balasubram, L Fleckenste, P Lulla, HE Heslop, J Vera, AM Leen Secreted Fas decoys enhance the antitumor activity of engineered and bystander T cells in Fas ligand-expressing solid tumors Oncogene, 2022-11-02;0(0):. 2022-11-02 [PMID: 36122411] (Human)
• T Bellón, O González-V, E Sendagorta, V Lerma, J Martínez D, C Martínez, G Servera, C González-H, L Cachafeiro, JA Lorente, R Cabañas, P Herranz, F de Abajo, PIELenRed IL-15/IL-15Ralpha in SJS/TEN: Relevant Expression of IL15 and IL15RA in Affected Skin Biomedicines, 2022-08-02;10(8):. 2022-08-02 [PMID: 36009415] (Human)
• A Rosewell S, C Porter, G Biegert, L Jatta, M Suzuki HydrAd: A Helper-Dependent Adenovirus Targeting Multiple Immune Pathways for Cancer Immunotherapy Oncogene, 2022-06-02;14(11):. 2022-06-02 [PMID: 35681750] (Human)
• LA Leroy, A Mac Donald, A Kandlur, D Bose, P Xiao, J Gagnon, F Villinger, Y Chebloune Cytokine Adjuvants IL-7 and IL-15 Improve Humoral Responses of a SHIV LentiDNA Vaccine in Animal Models Vaccines, 2022-03-17;10(3):. 2022-03-17 [PMID: 35335093] (Human)
• AK Singh, N Kasarpalka, S Bhowmick, G Paradkar, M Talreja, K Shah, A Tiwari, H Palav, S Kaginkar, R Kulkarni, A Patil, V Kalsurkar, S Agrawal, J Shastri, R Dere, R Bharmal, SD Mahale, VM Bhor, V Patel Opposing roles for sMAdCAM and IL-15 in COVID-19 associated cellular immune pathology Journal of leukocyte biology, 2022-01-24;0(0):. 2022-01-24 [PMID: 35075682] (Human)
• GR Kathamuthu, K Moideen, R Sridhar, D Baskaran, S Babu Altered plasma levels of &betaC and &gammaC chain cytokines and post-treatment modulation in tuberculous lymphadenitis Cytokine, 2020-12-17;138(0):155405. 2020-12-17 [PMID: 33341600] (Human)
• A Suhail, ZA Rizvi, P Mujagond, SA Ali, P Gaur, M Singh, V Ahuja, A Awasthi, CV Srikanth DeSUMOylase SENP7-Mediated Epithelial Signaling Triggers Intestinal Inflammation via Expansion of Gamma-Delta T Cells Cell Rep, 2019-12-10;29(11):3522-3538.e7. 2019-12-10 [PMID: 31825833] (Human)
• Ewa Kuca-Warna, Weronika Kurowska, Monika Prochorec, Anna Radzikows, Tomasz Burakowsk, Urszula Skalska, Magdalena Massalska, Magdalena Pleba?czy, Barbara Ma?dyk-No, Iwona S?owi?ska, Robert Gasik, W?odzimierz Ma?li?ski Rheumatoid arthritis bone marrow environment supports Th17 response. Arthritis Research & Therapy, 2017-12-08;0(0):1478-6362. 2017-12-08 [PMID: 29216915] (Human)
• Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways. J Immunol, 2015-08-14;195(6):2818-28. 2015-08-14 [PMID: 26276870] (Human)
• Marra P, Mathew S, Grigoriadis A, Wu Y, Kyle-Cezar F, Watkins J, Rashid M, De Rinaldis E, Hessey S, Gazinska P, Hayday A, Tutt A IL15RA drives antagonistic mechanisms of cancer development and immune control in lymphocyte-enriched triple-negative breast cancers. Cancer Res, 2014-06-30;74(17):4908-21. 2014-06-30 [PMID: 24980552] (Human)
• Barra NG, Reid S, MacKenzie R Interleukin-15 contributes to the regulation of murine adipose tissue and human adipocytes. Obesity (Silver Spring), 2009-12-17;18(8):1601-7. 2009-12-17 [PMID: 20019685] (Human)
• Hirosako S, Goto E, Fujii K, Tsumori K, Hirata N, Tsumura S, Kamohara H, Kohrogi H Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells. Clin. Exp. Immunol., 2008-11-26;155(2):266-74. 2008-11-26 [PMID: 19040600] (Human)
• Duitman EH, Orinska Z, Bulanova E, Paus R, Bulfone-Paus S How a cytokine is chaperoned through the secretory pathway by complexing with its own receptor: lessons from interleukin-15 (IL-15)/IL-15 receptor alpha. Mol. Cell. Biol., 2008-05-27;28(15):4851-61. 2008-05-27 [PMID: 18505820] (Human)
• Monti P, Scirpoli M, Maffi P, Ghidoli N, De Taddeo F, Bertuzzi F, Piemonti L, Falcone M, Secchi A, Bonifacio E Islet transplantation in patients with autoimmune diabetes induces homeostatic cytokines that expand autoreactive memory T cells. J. Clin. Invest., 2008-05-01;118(5):1806-14. 2008-05-01 [PMID: 18431516] (Human)
• Bulanova E, Budagian V, Duitman E, Orinska Z, Krause H, Ruckert R, Reiling N, Bulfone-Paus S Soluble Interleukin IL-15Ralpha is generated by alternative splicing or proteolytic cleavage and forms functional complexes with IL-15. J. Biol. Chem., 2007-02-27;282(18):13167-79. 2007-02-27 [PMID: 17327231] (Human)
• Miranda-Carus ME, Benito-Miguel M, Llamas MA, Balsa A, Martin-Mola E Human T cells constitutively express IL-15 that promotes ex vivo T cell homeostatic proliferation through autocrine/juxtacrine loops. J. Immunol., 2005-09-15;175(6):3656-62. 2005-09-15 [PMID: 16148110] (Human)
• Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate. J. Immunol., 2004-07-15;173(2):1463-76. 2004-07-15 [PMID: 15240743] (Human)
• Groh V, Bruhl A, El-Gabalawy H, Nelson JL, Spies T Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis. Proc. Natl. Acad. Sci. U.S.A., 2003-07-23;100(16):9452-7. 2003-07-23 [PMID: 12878725] (Human)

FAQs for IL-15 (DY247). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL15