Human GM-CSF Quantikine HS ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human GM-CSF Quantikine HS ELISA Kit Summary

• Background: The Quantikine HS Human GM-CSF Immunoassay is a 6.5 hour solid phase ELISA designed to measure human GM-CSF in serum, plasma, and urine. It contains E. coli-expressed recombinant human GM-CSF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural GM-CSF showed linear curves that were par...allel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GM-CSF.
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• Specificity: Natural and recombinant human GM-CSF
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CSF2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human GM-CSF Quantikine HS ELISA Kit

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.

Publications for GM-CSF (HSGM0)(11)

Publications using HSGM0

• Y Luo, QN Yan, WZ Wu, FY Luo Decreased Count and Dysfunction of Circulating EPCs in Postmenopausal Hypercholesterolemic Females via Reducing NO Production Stem Cells Int, 2018-04-11;2018(0):2543847. 2018-04-11 [PMID: 29760721] (Human)
• K Tanisawa, K Suzuki, S Ma, S Kondo, S Okugawa, M Higuchi Effects of Ingestion of Different Amounts of Carbohydrate after Endurance Exercise on Circulating Cytokines and Markers of Neutrophil Activation Antioxidants (Basel), 2018-04-02;7(4):. 2018-04-02 [PMID: 29614808] (Human)
• F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression Cancer Res., 2017-07-28;0(0):. 2017-07-28 [PMID: 28754674] (Mouse)
• SP Buchbinder, NA Grunenberg, BJ Sanchez, KE Seaton, G Ferrari, MA Moody, N Frahm, DC Montefiori, CM Hay, PA Goepfert, LR Baden, HL Robinson, X Yu, PB Gilbert, MJ McElrath, Y Huang, GD Tomaras Immunogenicity of a novel Clade B HIV-1 vaccine combination: Results of phase 1 randomized placebo controlled trial of an HIV-1 GM-CSF-expressing DNA prime with a modified vaccinia Ankara vaccine boost in healthy HIV-1 uninfected adults PLoS ONE, 2017-07-20;12(7):e0179597. 2017-07-20 [PMID: 28727817] (Human)
• Lipson E, Sharfman W, Chen S, McMiller T, Pritchard T, Salas J, Sartorius-Mergenthaler S, Freed I, Ravi S, Wang H, Luber B, Sproul J, Taube J, Pardoll D, Topalian S Safety and immunologic correlates of Melanoma GVAX, a GM-CSF secreting allogeneic melanoma cell vaccine administered in the adjuvant setting. J Transl Med, 2015-07-05;13(0):214. 2015-07-05 [PMID: 26143264] (Human)
• Paine R, Standiford TJ, Dechert RE, Moss M, Martin GS, Rosenberg AL, Thannickal VJ, Burnham EL, Brown MB, Hyzy RC A randomized trial of recombinant human granulocyte-macrophage colony stimulating factor for patients with acute lung injury. Crit. Care Med., 2012-01-01;40(1):90-7. 2012-01-01 [PMID: 21926600] (Human)
• Emens LA, Asquith JM, Leatherman JM, Kobrin BJ, Petrik S, Laiko M, Levi J, Daphtary MM, Biedrzycki B, Wolff AC, Stearns V, Disis ML, Ye X, Piantadosi S, Fetting JH, Davidson NE, Jaffee EM Timed sequential treatment with cyclophosphamide, doxorubicin, and an allogeneic granulocyte-macrophage colony-stimulating factor-secreting breast tumor vaccine: a chemotherapy dose-ranging factorial study of safety and immune activation. J. Clin. Oncol., 2009-10-05;27(35):5911-8. 2009-10-05 [PMID: 19805669] (Human)
• Grann VR, Ziv E, Joseph CK, Neugut AI, Wei Y, Jacobson JS, Horwitz MS, Bowman N, Beckmann K, Hershman DL Duffy (Fy), DARC, and neutropenia among women from the United States, Europe and the Caribbean. Br. J. Haematol., 2008-08-15;143(2):288-93. 2008-08-15 [PMID: 18710383] (Human)
• Grann VR, Bowman N, Joseph C, Wei Y, Horwitz MS, Jacobson JS, Santella RP, Hershman DL Neutropenia in 6 ethnic groups from the Caribbean and the U.S. Cancer, 2008-08-15;113(4):854-60. 2008-08-15 [PMID: 18543314] (Human)
• Rafat N, Hanusch C, Brinkkoetter PT, Schulte J, Brade J, Zijlstra JG, van der Woude FJ, van Ackern K, Yard BA, Beck GCh Increased circulating endothelial progenitor cells in septic patients: correlation with survival. Crit. Care Med., 2007-07-01;35(7):1677-84. 2007-07-01 [PMID: 17522579] (Human)
• Kramer BW, Joshi SN, Moss TJ, Newnham JP, Sindelar R, Jobe AH, Kallapur SG Endotoxin-induced maturation of monocytes in preterm fetal sheep lung. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-05-18;293(2):L345-53. 2007-05-18 [PMID: 17513458] (Ovine)

FAQs for GM-CSF (HSGM0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CSF2