Human EGF Quantikine ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Human EGF Quantikine ELISA Kit Summary

Background
The Quantikine Human EGF Immunoassay kit is a 3.5 or 4.5 hour solid phase ELISA designed to measure EGF levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human EGF and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human EGF. Results obtained measuring natural human EGF showe...d linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural EGF.
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Specificity
Natural and recombinant human EGF
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
EGF

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DEG00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human EGF Quantikine ELISA Kit

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for EGF (DEG00)(57)

We have publications tested in 3 confirmed species: Human, Rat, Plant - Arabidopsis.


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Human
(55)
Rat
(1)
Plant - Arabidopsis
(1)
All Species
Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using DEG00 Applications Species
S Di Marco, S Riccitelli, M Di Paolo, E Campos, M Buzzi, S Bisti, P Versura Cord Blood Serum (CBS)-Based Eye Drops Modulate Light-Induced Neurodegeneration in Albino Rat Retinas Biomolecules, 2020;10(5):. 2020 [PMID: 32354031] (Human) Human
Z Dong, J Zou, J Li, Y Pang, Y Liu, C Deng, F Chen, H Cui MYST1/KAT8 contributes to tumor progression by activating EGFR signaling in glioblastoma cells Cancer Med, 2019;0(0):. 2019 [PMID: 31691527] (Human) Human
ASMR Haque, M Moriyama, K Kubota, N Ishiguro, M Sakamoto, A Chinju, K Mochizuki, T Sakamoto, N Kaneko, R Munemura, T Maehara, A Tanaka, JN Hayashida, S Kawano, T Kiyoshima, S Nakamura CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production Sci Rep, 2019;9(1):14611. 2019 [PMID: 31601953] (Human) Human
ZJ Liang, X Lu, DD Zhu, XL Yi, FX Wu, N He, C Tang, CY Wei, HM Li Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro Cell Transplant, 2019;0(0):9636897198256. 2019 [PMID: 30675799] (Human) Human
B Satirapoj, R Dispan, P Radinahame, C Kitiyakara Urinary epidermal growth factor, monocyte chemoattractant protein-1 or their ratio as predictors for rapid loss of renal function in type 2 diabetic patients with diabetic kidney disease BMC Nephrol, 2018;19(1):246. 2018 [PMID: 30241508] (Human) Human
F Agostini, M Ruzza, D Corpillo, L Biondi, E Acquadro, B Canepa, A Viale, M Battiston, F Serra, S Aime, M Mazzucato 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures PLoS ONE, 2018;13(9):e0203048. 2018 [PMID: 30188924] (Human) Human
C Saury, A Lardenois, C Schleder, I Leroux, B Lieubeau, L David, M Charrier, L Guével, S Viau, B Delorme, K Rouger Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches Stem Cell Res Ther, 2018;9(1):128. 2018 [PMID: 29720259] (Human) Human
P Xing, H Wang, S Yang, X Han, Y Sun, Y Shi Therapeutic cancer vaccine: phase I clinical tolerance study of Hu-rhEGF-rP64k/Mont in patients with newly diagnosed advanced non-small cell lung cancer BMC Immunol., 2018;19(1):14. 2018 [PMID: 29661145] (Human) Human
N Azuma, Y Katada, H Sano Deterioration in saliva quality in patients with Sj�gren's syndrome: impact of decrease in salivary epidermal growth factor on the severity of intraoral manifestations Inflamm Regen, 2018;38(0):6. 2018 [PMID: 29657585] (Human) Human
Jan Pierce, Eric Benedetti, Amber Preslar, Pam Jacobson, Ping Jin, David F Stroncek, Jo-Anna Reems Comparative analyses of industrial-scale human platelet lysate preparations. Transfusion, 2017;0(0):1537-2995. 2017 [PMID: 28990195] (Human) Human
Show All 57 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for EGF (DEG00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DEG00
HRP DEG00
HRP DEG00

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Bioinformatics Tool for EGF (DEG00)

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Blogs on EGF.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

Myosin is More than Just a Heavy Lifter
Myosin is a well-known, hexameric molecular motor that is a key cytoskeletal component. It consists of a pair of myosin heavy chain subunits (MHC), a pair of essential myosin light chain subunits (MLC), and a pair of regulatory light chain subunits (R...  Read full blog post.

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Bioinformatics

Gene Symbol EGF