Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit Summary

• Background: The Quantikine Human BLC/BCA-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BLC/BCA-1 in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human BLC/BCA-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BLC/BCA-1 showed linear curves that were parallel to the... standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring BLC/BCA-1.
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• Specificity: Natural and recombinant human BLC/BCA-1
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CXCL13

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

• ANGIE
• ANGIE2
• B cell-attracting chemokine 1
• B lymphocyte chemoattractant
• BCA1
• BCA-1
• BCA1B-cell chemoattractant
• BCA-1CXC chemokine BLC
• B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
• BLC
• BLCSmall-inducible cytokine B13
• BLR1L
• B-lymphocyte chemoattractant
• chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
• chemokine (C-X-C motif) ligand 13
• C-X-C motif chemokine 13
• CXCL13
• SCYB13
• SCYB13B-cell-attracting chemokine 1
• small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naïve B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer’s patch development.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CXCL13/BLC/BCA-1 (DCX130)(46)

Publications using DCX130

• SK Pierson, L Katz, R Williams, M Mumau, M Gonzalez, S Guzman, A Rubenstein, AB Oromendia, P Beineke, A Fosså, F van Rhee, DC Fajgenbaum CXCL13 is a predictive biomarker in idiopathic multicentric Castleman disease Nature Communications, 2022;13(1):7236. 2022 [PMID: 36433996] (Human)
• J Ruder, G Dinner, A Maceski, E Berenjeno-, AM Müller, I Jelcic, J Kuhle, R Martin Dynamics of Inflammatory and Neurodegenerative Biomarkers after Autologous Hematopoietic Stem Cell Transplantation in Multiple Sclerosis International Journal of Molecular Sciences, 2022;23(18):. 2022 [PMID: 36142860] (Human)
• AG Goubet, L Lordello, C Alves Cost, I Peguillet, M Gazzano, MD Mbogning-F, C Thelemaque, C Lebacle, C Thibault, F Audenet, G Pignot, G Gravis, C Helissey, L Campedel, M Roupret, E Xylinas, I Ouzaid, A Dubuisson, M Mazzenga, C Flament, P Ly, V Marty, N Signolle, A Sauvat, T Sbarrato, M Filahi, C Davin, G Haddad, J Bou Khalil, C Bleriot, FX Danlos, G Dunsmore, K Mulder, A Silvin, T Raoult, B Archambaud, S Belhechmi, I Gomperts B, N Cayet, M Moya-Nilge, A Mallet, R Daillere, E Rouleau, C Radulescu, Y Allory, J Fieschi, M Rouanne, F Ginhoux, G Le Teuff, L Derosa, A Marabelle, J VAN Dorp, N VAN Dijk, MS van der He, B Besse, F Andre, M Merad, G Kroemer, JY Scoazec, L Zitvogel, Y Loriot Escherichia coli-specific CXCL13-producing TFH are associated with clinical efficacy of neoadjuvant PD-1 blockade against muscle-invasive bladder cancer Cancer Discovery, 2022;0(0):. 2022 [PMID: 35929803] (Human)
• J Jeon, J Park, HJ Boo, KE Yang, CJ Lee, JE Lee, K Kim, GY Kwon, W Huh, DJ Kim, YG Kim, HR Jang Clinical value of urinary cytokines/chemokines as prognostic markers in patients with crescentic glomerulonephritis Oncogene, 2022;12(1):10221. 2022 [PMID: 35715470] (Human)
• M Ukita, J Hamanishi, H Yoshitomi, K Yamanoi, S Takamatsu, A Ueda, H Suzuki, Y Hosoe, Y Furutake, M Taki, K Abiko, K Yamaguchi, H Nakai, T Baba, N Matsumura, A Yoshizawa, H Ueno, M Mandai CXCL13-producing CD4+ T cells accumulate in early phase of tertiary lymphoid structures in ovarian cancer JCI Insight, 2022;0(0):. 2022 [PMID: 35552285] (Human)
• BS Cox, K Alharshawi, I Mena-Palom, WP Lafuse, ME Ariza EBV/HHV-6A dUTPases contribute to myalgic encephalomyelitis/chronic fatigue syndrome pathophysiology by enhancing TFH cell differentiation and extrafollicular activities JCI Insight, 2022;7(11):. 2022 [PMID: 35482424] (Human)
• T Robinson, A Abdelhak, T Bose, E Meinl, M Otto, UK Zettl, R Dersch, H Tumani, S Rauer, A Huss Cerebrospinal Fluid Biomarkers in Relation to MRZ Reaction Status in Primary Progressive Multiple Sclerosis Cells, 2020;9(12):. 2020 [PMID: 33255854] (Human)
• M Sivina, L Xiao, E Kim, A Vaca, SS Chen, MJ Keating, A Ferrajoli, Z Estrov, N Jain, WG Wierda, X Huang, N Chiorazzi, JA Burger CXCL13 plasma levels function as a biomarker for disease activity in patients with chronic lymphocytic leukemia Leukemia, 2020;0(0):. 2020 [PMID: 33087831] (Human)
• P Körtvelyes, A Goihl, K Guttek, B Schraven, H Prüss, D Reinhold Serum and CSF cytokine levels mirror different neuroimmunological mechanisms in patients with LGI1 and Caspr2 encephalitis Cytokine, 2020;135(0):155226. 2020 [PMID: 32799011] (Human)
• F Saberi Hos, PM Kolijn, D Casabonne, A Nieters, M Solans, S Naudin, P Ferrari, JD Mckay, E Weiderpass, V Perduca, C Besson, FR Mancini, G Masala, V Krogh, F Ricceri, JM Huerta, D Petrova, N Sala, A Trichopoul, A Karakatsan, C La Vecchia, R Kaaks, F Canzian, D Aune, H Boeing, MB Schulze, A Perez-Corn, AW Langerak, VHJ van der Ve, R Vermeulen Mediating effect of soluble B-cell activation immune markers on the association between anthropometric and lifestyle factors and lymphoma development Sci Rep, 2020;10(1):13814. 2020 [PMID: 32796953] (Human)
• F Saberi Hos, PM Kolijn, D Casabonne, A Nieters, M Solans, S Naudin, P Ferrari, JD Mckay, E Weiderpass, V Perduca, C Besson, FR Mancini, G Masala, V Krogh, F Ricceri, JM Huerta, D Petrova, N Sala, A Trichopoul, A Karakatsan, C La Vecchia, R Kaaks, F Canzian, D Aune, H Boeing, MB Schulze, A Perez-Corn, AW Langerak, VHJ van der Ve, R Vermeulen Mediating effect of soluble B-cell activation immune markers on the association between anthropometric and lifestyle factors and lymphoma development Sci Rep, 2020;10(1):13814. 2020 [PMID: 32796953] (Human)
• AM Globig, NP Sommer, K Wild, J Schardey, K Zoldan, AK Thomann, LA Schulte, R Schreiner, W Reindl, J Klaus, CM Schempp, M Hofmann, R Thimme, T Boettler, P Hasselblat Ustekinumab inhibits T follicular helper cell differentiation in patients with Crohn's disease Cell Mol Gastroenterol Hepatol, 2020;0(0):. 2020 [PMID: 32679193] (Human)
• DM Muema, NA Akilimali, OC Ndumnego, SS Rasehlo, R Durgiah, DBA Ojwach, N Ismail, M Dong, A Moodley, KL Dong, ZM Ndhlovu, JM Mabuka, BD Walker, JK Mann, T Ndung'u Association between the cytokine storm, immune cell dynamics, and viral replicative capacity in hyperacute HIV infection BMC Med, 2020;18(1):81. 2020 [PMID: 32209092] (Human)
• S Miyajima, K Shigehara, R Kamekura, H Takaki, H Yabe, I Ikegami, Y Asai, H Nishikiori, H Chiba, E Uno, H Takahashi, S Ichimiya Activated circulating T follicular helper cells and skewing of T follicular helper 2�cells are down-regulated by treatment including an inhaled corticosteroid in patients with allergic asthma Allergol Int, 2019;0(0):. 2019 [PMID: 31648923] (Human)
• A Morou, E Brunet-Rat, M Dubé, R Charlebois, E Mercier, S Darko, N Brassard, K Nganou-Mak, S Arumugam, G Gendron-Le, L Yang, J Niessl, AE Baxter, JM Billingsle, PA Rajakumar, F Lefebvre, RP Johnson, C Tremblay, JP Routy, RT Wyatt, A Finzi, DC Douek, DE Kaufmann Altered differentiation is central to HIV-specific CD4+ T cell dysfunction in progressive disease Nat. Immunol., 2019;20(8):1059-1070. 2019 [PMID: 31308541] (Human)
• A Ardain, R Domingo-Go, S Das, SW Kazer, NC Howard, A Singh, M Ahmed, S Nhamoyebon, J Rangel-Mor, P Ogongo, L Lu, D Ramsuran, M de la Luz, T K Ulland, M Darby, E Park, F Karim, L Melocchi, R Madansein, KJ Dullabh, M Dunlap, N Marin-Agud, T Ebihara, T Ndung'u, D Kaushal, AS Pym, JK Kolls, A Steyn, J Zúñiga, W Horsnell, WM Yokoyama, AK Shalek, HN Kløverpris, M Colonna, A Leslie, SA Khader Group 3 innate lymphoid cells mediate early protective immunity against tuberculosis Nature, 2019;570(7762):528-532. 2019 [PMID: 31168092] (Human)
• EJ Im, CH Lee, PG Moon, GG Rangaswamy, B Lee, JM Lee, JC Lee, JG Jee, JS Bae, TK Kwon, KW Kang, MS Jeong, JE Lee, HS Jung, HJ Ro, S Jun, W Kang, SY Seo, YE Cho, BJ Song, MC Baek Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A Nat Commun, 2019;10(1):1387. 2019 [PMID: 30918259] (Human)
• SI Richardson, AW Chung, H Natarajan, B Mabvakure, NN Mkhize, N Garrett, S Abdool Kar, PL Moore, ME Ackerman, G Alter, L Morris HIV-specific Fc effector function early in infection predicts the development of broadly neutralizing antibodies PLoS Pathog., 2018;14(4):e1006987. 2018 [PMID: 29630668] (Human)
• A Pietikäine, J Oksi, J Hytönen Point-of-care testing for CXCL13 in Lyme neuroborreliosis Diagn. Microbiol. Infect. Dis., 2018;0(0):. 2018 [PMID: 29567125] (Human)
• RM Paris, LG Milagres, E Moysi, JF Okulicz, BK Agan, A Ganesan, C Petrovas, RA Koup Lower Baseline Germinal Center Activity and Preserved Th1 Immunity Are Associated With Hepatitis B Vaccine Response in Treated HIV Infection Pathog Immun, 2017;2(1):66-88. 2017 [PMID: 28580437] (Human)
• S Wang, T Yang, J Wan, Y Zhang, Y Fan Elevated C-X-C motif ligand 13 and B-cell-activating factor levels in neuromyelitis optica during remission Brain Behav, 2017;7(4):e00648. 2017 [PMID: 28413701] (Human)
• P Gyllemark, P Forsberg, J Ernerudh, AJ Henningsso Intrathecal Th17- and B cell-associated cytokine and chemokine responses in relation to clinical outcome in Lyme neuroborreliosis: a large retrospective study J Neuroinflammation, 2017;14(1):27. 2017 [PMID: 28148307] (Human)
• M Puthenpara, L Federle, S Miante, A Zito, E Toffanin, S Ruggero, M Ermani, S Pravato, D Poggiali, P Perini, F Rinaldi, P Gallo BAFF Index and CXCL13 levels in the cerebrospinal fluid associate respectively with intrathecal IgG synthesis and cortical atrophy in multiple sclerosis at clinical onset J Neuroinflammation, 2017;14(1):11. 2017 [PMID: 28095856] (Human)
• CXCL13 is a plasma biomarker of germinal center activity Proc. Natl. Acad. Sci. U.S.A., 2016;113(10):2702-7. 2016 [PMID: 26908875] (Human)
• Epeldegui M, Lee J, Martinez A, Widney D, Magpantay L, Regidor D, Mitsuyasu R, Sparano J, Ambinder R, Martinez-Maza O Predictive Value of Cytokines and Immune Activation Biomarkers in AIDS-Related Non-Hodgkin Lymphoma Treated with Rituximab plus Infusional EPOCH (AMC-034 trial). Clin Cancer Res, 2016;22(2):328-36. 2016 [PMID: 26384320] (Human)
• Stilund M, Gjelstrup M, Petersen T, Moller H, Rasmussen P, Christensen T Biomarkers of inflammation and axonal degeneration/damage in patients with newly diagnosed multiple sclerosis: contributions of the soluble CD163 CSF/serum ratio to a biomarker panel. PLoS ONE, 2015;10(4):e0119681. 2015 [PMID: 25860354] (Human)
• Cohen K, Dugast A, Alter G, McElrath M, Stamatatos L HIV-1 single-stranded RNA induces CXCL13 secretion in human monocytes via TLR7 activation and plasmacytoid dendritic cell-derived type I IFN. J Immunol, 2015;194(6):2769-75. 2015 [PMID: 25667414] (Human)
• Hytonen J, Kortela E, Waris M, Puustinen J, Salo J, Oksi J CXCL13 and neopterin concentrations in cerebrospinal fluid of patients with Lyme neuroborreliosis and other diseases that cause neuroinflammation. J Neuroinflammation, 2014;11(0):103. 2014 [PMID: 24920219] (Human)
• Senel M, Tumani H, Lauda F, Presslauer S, Mojib-Yezdani R, Otto M, Brettschneider J Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis. PLoS ONE, 2014;9(4):e88680. 2014 [PMID: 24695382] (Human)
• Pranzatelli , Michael, Tate , Elizabet, McGee , Nathan R, Travelstead , Anna L, Colliver , Jerry A, Ness , Jayne M, Ransohoff , Richard BAFF/APRIL system in pediatric OMS: relation to severity, neuroinflammation, and immunotherapy. J Neuroinflammation, 2013;10(0):10. 2013 [PMID: 23324534] (Human)
• Tiberti N, Hainard A, Lejon V, Courtioux B, Matovu E, Enyaru JC, Robin X, Turck N, Kristensson K, Ngoyi DM, Vatunga GM, Krishna S, Buscher P, Bisser S, Ndung'u JM, Sanchez JC Cerebrospinal Fluid Neopterin as Marker of the Meningo-Encephalitic Stage of Trypanosoma brucei gambiense Sleeping Sickness. PLoS ONE, 2012;7(7):e40909. 2012 [PMID: 22815865] (Human)
• Brettschneider J, Czerwoniak A, Senel M The chemokine CXCL13 is a prognostic marker in clinically isolated syndrome (CIS). PLoS ONE, 2010;5(8):e11986. 2010 [PMID: 20700489] (Human)
• Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion. Cancer Lett., 2009;283(1):29-35. 2009 [PMID: 19375853] (Human)
• Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease. J. Immunol., 2009;182(6):3919-27. 2009 [PMID: 19265171] (Human)
• Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients. Br. J. Cancer, 2008;99(6):930-8. 2008 [PMID: 18781150] (Human)
• Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection. Blood, 2008;112(12):4401-10. 2008 [PMID: 18780835] (Human)
• Rioja I, Hughes FJ, Sharp CH, Warnock LC, Montgomery DS, Akil M, Wilson AG, Binks MH, Dickson MC Potential novel biomarkers of disease activity in rheumatoid arthritis patients: CXCL13, CCL23, transforming growth factor alpha, tumor necrosis factor receptor superfamily member 9, and macrophage colony-stimulating factor. Arthritis Rheum., 2008;58(8):2257-67. 2008 [PMID: 18668547] (Human)
• Sansonno D, Tucci FA, Troiani L, Lauletta G, Montrone M, Conteduca V, Sansonno L, Dammacco F Increased serum levels of the chemokine CXCL13 and up-regulation of its gene expression are distinctive features of HCV-related cryoglobulinemia and correlate with active cutaneous vasculitis. Blood, 2008;112(5):1620-7. 2008 [PMID: 18550853] (Human)
• Aloisi F, Columba-Cabezas S, Franciotta D, Rosicarelli B, Magliozzi R, Reynolds R, Ambrosini E, Coccia E, Salvetti M, Serafini B Lymphoid chemokines in chronic neuroinflammation. J. Neuroimmunol., 2008;198(1):106-12. 2008 [PMID: 18539341] (Human)
• Lim HW, Kim CH Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue. J. Immunol., 2007;179(11):7448-56. 2007 [PMID: 18025189] (Human)
• Burkle A, Niedermeier M, Schmitt-Graff A, Wierda WG, Keating MJ, Burger JA Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia. Blood, 2007;110(9):3316-25. 2007 [PMID: 17652619] (Human)
• Shin JJ, Glickstein LJ, Steere AC High levels of inflammatory chemokines and cytokines in joint fluid and synovial tissue throughout the course of antibiotic-refractory lyme arthritis. Arthritis Rheum., 2007;56(4):1325-35. 2007 [PMID: 17393419] (Human)
• Munoz-Fernandez R, Blanco FJ, Frecha C, Martin F, Kimatrai M, Abadia-Molina AC, Garcia-Pacheco JM, Olivares EG Follicular dendritic cells are related to bone marrow stromal cell progenitors and to myofibroblasts. J. Immunol., 2006;177(1):280-9. 2006 [PMID: 16785523] (Human)
• Lim HW, Hillsamer P, Kim CH Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. J. Clin. Invest., 2004;114(11):1640-9. 2004 [PMID: 15578096] (Human)
• Casazza S, Zappia E, Pistorio A, Gambini C Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis. Proc. Natl. Acad. Sci. U.S.A., 2004;101(30):11064-9. 2004 [PMID: 15263096] (Human)
• Lisignoli G, Cristino S, Toneguzzi S, Grassi F, Piacentini A, Cavallo C, Facchini A, Mariani E IL1beta and TNFalpha differently modulate CXCL13 chemokine in stromal cells and osteoblasts isolated from osteoarthritis patients: evidence of changes associated to cell maturation. Exp. Gerontol., 2004;39(4):659-65. 2004 [PMID: 15050303] (Human)

Bioinformatics

• Gene Symbol: CXCL13