Human CNTF Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CNTF Quantikine ELISA Kit Summary

• Background: The Quantikine Human CNTF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure recombinant human CNTF in cell culture supernates, serum, and plasma. It contains recombinant human CNTF and has been shown to accurately quantitate the recombinant factor.
• Specificity: Recombinant human CNTF
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CNTF

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CNTF Quantikine ELISA Kit

• ciliary neurotrophic factor
• CNTF
• HCNTF

Background

Ciliary neurotrophic factor (CNTF) is structurally related to IL-6, IL-11, LIF, CLC, and OSM. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types, including dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CNTF (DNT00)(12)

Publications using DNT00

• Kauper, K;Nystuen, A;Orecchio, L;Gonzalez-Lopez, E;Lee, A;Duncan, JL;Stewart, JM;Aaberg, T; Long-Term Durability of Ciliary Neurotrophic Factor-Releasing Revakinagene Taroretcel-lwey in Individuals With Retinal Degenerative Disorders Investigative ophthalmology & visual science 2025-08-01 [PMID: 40757774] (Human)
• TA Druzhkova, AA Yakovlev, FK Rider, MS Zinchuk, AB Guekht, NV Gulyaeva Elevated Serum Cortisol Levels in Patients with Focal Epilepsy, Depression, and Comorbid Epilepsy and Depression International Journal of Molecular Sciences, 2022-09-08;23(18):. 2022-09-08 [PMID: 36142325] (Human)
• K Terawaki, Y Kashiwase, M Uzu, M Nonaka, Y Sawada, K Miyano, Y Higami, K Yanagihara, M Yamamoto, Y Uezono Leukemia inhibitory factor via the Toll-like receptor 5 signaling pathway involves aggravation of cachexia induced by human gastric cancer-derived 85As2 cells in rats Oncotarget, 2018-10-05;9(78):34748-34764. 2018-10-05 [PMID: 30410674] (Human)
• AA Shpak, AB Guekht, TA Druzhkova, KI Kozlova, NV Gulyaeva Ciliary neurotrophic factor in patients with primary open-angle glaucoma and age-related cataract Mol. Vis., 2017-11-17;23(0):799-809. 2017-11-17 [PMID: 29225456] (Human)
• L Xu, C Zhang, L Liu, Y Zhang, Q Wang, J Wang, Y Liu, Z Su Purification and characterization of a long-acting ciliary neurotrophic factor via genetically fused with an albumin-binding domain Protein Expr. Purif., 2017-07-12;0(0):. 2017-07-12 [PMID: 28711730] (Rat)
• C Zhang, R Yu, Z Li, C Feng, Q Wang, Y Liu, Z Su Development of long-acting ciliary neurotrophic factor by site-specific conjugation with different-sized polyethylene glycols and transferrin Int J Pharm, 2017-06-23;0(0):. 2017-06-23 [PMID: 28652173] (Human)
• Sieving PA, Caruso RC, Tao W, Coleman HR, Thompson DJ, Fullmer KR, Bush RA Ciliary neurotrophic factor (CNTF) for human retinal degeneration: phase I trial of CNTF delivered by encapsulated cell intraocular implants. Proc. Natl. Acad. Sci. U.S.A., 2006-02-27;103(10):3896-901. 2006-02-27 [PMID: 16505355] (Human)
• Saigo Y, Abe T, Hojo M, Tomita H, Sugano E, Tamai M Transplantation of transduced retinal pigment epithelium in rats. Invest. Ophthalmol. Vis. Sci., 2004-06-01;45(6):1996-2004. 2004-06-01 [PMID: 15161868] (Rat)
• Truckenmiller ME, Vawter MP, Zhang P, Conejero-Goldberg C, Dillon-Carter O, Morales N, Cheadle C, Becker KG, Freed WJ AF5, a CNS cell line immortalized with an N-terminal fragment of SV40 large T: growth, differentiation, genetic stability, and gene expression. Exp. Neurol., 2002-06-01;175(0):318. 2002-06-01 [PMID: 12061863] (Rat)
• Mittoux V, Joseph JM, Conde F, Palfi S, Dautry C, Poyot T, Bloch J, Deglon N, Ouary S, Nimchinsky EA, Brouillet E, Hof PR, Peschanski M, Aebischer P, Hantraye P Restoration of cognitive and motor functions by ciliary neurotrophic factor in a primate model of Huntington's disease. Hum. Gene Ther., 2000-05-20;11(8):1177-87. 2000-05-20 [PMID: 10834619] (Human)
• Yang C, Xiao L, Tongren JE, Sullivan J, Lal AA, Collins WE Cytokine production in rhesus monkeys infected with Plasmodium coatneyi. Am. J. Trop. Med. Hyg., 1999-08-01;61(2):226-9. 1999-08-01 [PMID: 10463671] (Primate)
• Anand P, Parrett A, Martin J, Zeman S, Foley P, Swash M, Leigh PN, Cedarbaum JM, Lindsay RM, Williams-Chestnut RE Regional changes of ciliary neurotrophic factor and nerve growth factor levels in post mortem spinal cord and cerebral cortex from patients with motor disease. Nat. Med., 1995-02-01;1(2):168-72. 1995-02-01 [PMID: 7585015] (Human)

FAQs for CNTF (DNT00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CNTF