Human CD25/IL-2 R alpha QuicKit ELISA

• Reactivity: Hu
• Conjugate: HRP

Human CD25/IL-2 R alpha QuicKit ELISA Summary

• Background: The Quantikine QuicKit Human CD25/IL-2 R alpha Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human IL-2 R alpha levels in cell culture supernates, serum, plasma, and cell lysates. It contains recombinant human IL-2 R alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-2 R alpha showed linear curves th...at were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-2 R alpha .
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• Specificity: Natural and recombinant human IL-2 R alpha .
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CD25/IL-2 R alpha QuicKit ELISA

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

CD25/IL-2 receptor alpha is a transmembrane protein that associates with IL-2 R beta/CD122 and the Common gamma Chain/CD132 on activated T cells and regulatory T cells (Treg). The Common gamma chain is a shared subunit in the receptors for IL-4, -7, -9, -15, and -21. IL-2 R alpha is required for mediating IL-2 induced effects including T cell proliferation, activation induced cell death (AICD) of autoreactive naive T cells, and the development of CD4+CD25+ Treg which contribute to peripheral T cell homeostasis. A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor.