Human CCL20/MIP-3 alpha DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CCL20/MIP-3 alpha DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL20

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL20/MIP-3 alpha DuoSet ELISA, 15 Plate

• beta chemokine exodus-1
• Beta-chemokine exodus-1
• CC chemokine LARC
• C-C motif chemokine 20
• CCL20
• chemokine (C-C motif) ligand 20
• CKb4
• exodus-1
• LARC
• LARCLiver and activation-regulated chemokine
• MIP3 alpha
• MIP-3 alpha
• MIP-3a
• MIP-3-alpha
• MIP3AMacrophage inflammatory protein 3 alpha
• SCYA20Small-inducible cytokine A20
• small inducible cytokine subfamily A (Cys-Cys), member 20
• ST38

Background

CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.

Publications for CCL20/MIP-3 alpha (DY360)(39)

Publications using DY360

• Brunner, M;Guggisberg, D;Sprecher, M;Pastva, O;Bürki, K;Houtman, M;Kreuzer, M;Krättli, SA;Jahnke, L;Roceri, M;Bruggmann, R;Elhai, M;Maurer, B;Marti, TM;Ospelt, C;Klein, K; Pro-Inflammatory Properties of Salivary Gland-Derived Fibroblasts-Implications in Sjögren's Disease Cells 2025-04-08 [PMID: 40277884] (Human)
• Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M; Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls PloS one 2024-07-15 [PMID: 39008499] (Human)
• Chick, HM;Rees, ME;Lewis, ML;Williams, LK;Bodger, O;Harris, LG;Rushton, S;Wilkinson, TS; Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses Biomedicines 2024-03-25 [PMID: 38672079] (Human)
• Kislat, A;Olah, P;Kuchner, M;Gerber, PA;Schrader, J;Meller, S;Homey, B; The Endogenous Dual Retinoid Receptor Agonist Alitretinoin Exhibits Immunoregulatory Functions on Antigen-Presenting Cells International journal of molecular sciences 2023-06-02 [PMID: 37298605] (Human)
• H Janbazacya, J van Bergen, S Varasteh, J Garssen, G Folkerts, S Braber Repeated exposure of bronchial epithelial cells to particular matter increases allergen-induced cytokine release and permeability Cytokine, 2022-04-08;154(0):155878. 2022-04-08 [PMID: 35405483] (Human)
• P Esteves, B Allard, A Celle, I Dupin, E Maurat, O Ousova, M Thumerel, JW Dupuy, T Leste-Lass, R Marthan, PO Girodet, T Trian, P Berger Asthmatic bronchial smooth muscle increases rhinovirus replication within the bronchial epithelium Cell Reports, 2022-03-29;38(13):110571. 2022-03-29 [PMID: 35354045] (Human)
• B Grüner, L Peters, A Hillenbran, P Vo beta berg, J Schweiker, EG Rollmann, LH Rodriguez, J Blumhardt, S Burkert, P Kern, C Köhler, PT Soboslay Echinococcus multilocularis specific antibody, systemic cytokine, and chemokine levels, as well as antigen-specific cellular responses in patients with progressive, stable, and cured alveolar echinococcosis: A 10-year follow-up PloS Neglected Tropical Diseases, 2022-02-02;16(2):e0010099. 2022-02-02 [PMID: 35108275] (Human)
• R Pohl, S Feder, EM Haberl, L Rein-Fisch, TS Weiss, M Spirk, A Bruckmann, N McMullen, CJ Sinal, C Buechler Chemerin Overexpression in the Liver Protects against Inflammation in Experimental Non-Alcoholic Steatohepatitis Biomedicines, 2022-01-07;10(1):. 2022-01-07 [PMID: 35052810] (Human)
• SR Bruno, A Kumar, ZF Mark, R Chandrasek, E Nakada, N Chamberlai, B Mihavics, J Walzer, J Cahoon, AE Dixon, B Cunniff, V Anathy DRP1-Mediated Mitochondrial Fission Regulates Lung Epithelial Response to Allergen International Journal of Molecular Sciences, 2021-10-15;22(20):. 2021-10-15 [PMID: 34681784] (Human)
• MS Shaban, C Müller, C Mayr-Buro, H Weiser, J Meier-Soel, BV Albert, A Weber, U Linne, T Hain, I Babayev, N Karl, N Hofmann, S Becker, S Herold, ML Schmitz, J Ziebuhr, M Kracht Multi-level inhibition of coronavirus replication by chemical ER stress Nature Communications, 2021-09-20;12(1):5536. 2021-09-20 [PMID: 34545074] (Human)
• S Gómez-Mele, FI García-Mac, T García-Mac, V Luna-Guerr, G Montero-Pe, I Túnez-Fiña, E Paz-Rojas Amino terminal recognition by a CCR6 chemokine receptor antibody blocks CCL20 signaling and IL-17 expression via beta-arrestin BMC biotechnology, 2021-07-05;21(1):41. 2021-07-05 [PMID: 34225700] (Human)
• HK Skovdahl, S Gopalakris, TD Svendsen, AVB Granlund, I Bakke, ZG Ginbot, S Thorsvik, A Flatberg, B Sporsheim, J Ostrop, TE Mollnes, AK Sandvik, T Bruland Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration Frontiers in Pharmacology, 2021-05-13;12(0):679741. 2021-05-13 [PMID: 34054553] (Human)
• GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation Biomolecules, 2021-02-21;11(2):. 2021-02-21 [PMID: 33669910] (Human)
• H Gunawardan, T Romero, N Yao, S Heidt, A Mulder, DA Elashoff, NM Valenzuela Tissue-specific endothelial cell heterogeneity contributes to unequal inflammatory responses Scientific Reports, 2021-01-21;11(1):1949. 2021-01-21 [PMID: 33479269] (Human)
• AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation PLoS Pathog., 2020-08-18;16(8):e1008781. 2020-08-18 [PMID: 32810179] (Human)
• T Zhuang, X Yi, J Chen, P Kang, X Chen, J Chen, T Cui, Y Chang, Z Ye, Q Ni, Y Wang, P Du, B Li, L Liu, Z Jian, K Li, T Gao, S Li, C Li Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion Cell Death Dis, 2020-06-12;11(6):453. 2020-06-12 [PMID: 32532953] (Human)
• FJ Ryan, AM Ahern, RS Fitzgerald, EJ Laserna-Me, EM Power, AG Clooney, KW O'Donoghue, PJ McMurdie, S Iwai, A Crits-Chri, D Sheehan, C Moran, B Flemer, AL Zomer, A Fanning, J O'Callagha, J Walton, A Temko, W Stack, L Jackson, SA Joyce, S Melgar, TZ DeSantis, JT Bell, F Shanahan, MJ Claesson Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease Nat Commun, 2020-03-23;11(1):1512. 2020-03-23 [PMID: 32251296] (Human)
• JA Woznicki, P Flood, M Bustamante, P Stamou, G Moloney, A Fanning, SA Zulquernai, J McCarthy, F Shanahan, S Melgar, K Nally Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-gamma and TNF-alpha in intestinal epithelial cells Cell Death Dis, 2020-01-27;11(1):68. 2020-01-27 [PMID: 31988296] (Human)
• D Martin-Gar, C Silva-Vilc, R Will, AH Enk, AS Lonsdorf Tumor-derived CCL20 affects B16 melanoma growth in mice J. Dermatol. Sci., 2019-12-13;0(0):. 2019-12-13 [PMID: 31883833] (Human)
• S Di Vincenz, IH Heijink, JA Noordhoek, C Cipollina, L Siena, A Bruno, M Ferraro, DS Postma, M Gjomarkaj, E Pace SIRT1/FoxO3 axis alteration leads to aberrant immune responses in bronchial epithelial cells J. Cell. Mol. Med., 2018-02-07;0(0):. 2018-02-07 [PMID: 29411515] (Human)
• JS Hähnlein, TH Ramwadhdoe, JF Semmelink, IY Choi, FH Berger, M Maas, DM Gerlag, PP Tak, TBH Geijtenbee, LGM van Baarse Distinctive expression of T cell guiding molecules in human autoimmune lymph node stromal cells upon TLR3 triggering Sci Rep, 2018-01-29;8(1):1736. 2018-01-29 [PMID: 29379035] (Human)
• Ewa Kuca-Warna, Weronika Kurowska, Monika Prochorec, Anna Radzikows, Tomasz Burakowsk, Urszula Skalska, Magdalena Massalska, Magdalena Pleba?czy, Barbara Ma?dyk-No, Iwona S?owi?ska, Robert Gasik, W?odzimierz Ma?li?ski Rheumatoid arthritis bone marrow environment supports Th17 response. Arthritis Research & Therapy, 2017-12-08;0(0):1478-6362. 2017-12-08 [PMID: 29216915] (Human)
• Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo IL-27 Modulates Chemokine Production in TNF-? -Stimulated Human Oral Epithelial Cells Cell. Physiol. Biochem., 2017-10-05;43(3):1198-1206. 2017-10-05 [PMID: 28977795] (Human)
• P Severino, DT Palomino, H Alvarenga, CB Almeida, DC Pasqualim, A Cury, PR Salvalaggi, AL De Vasconc, MC Andrade, T Aloia, S Bromberg, LV Rizzo, FA Rocha, LC Marti Human Lymph Node-Derived Fibroblastic and Double-Negative Reticular Cells Alter Their Chemokines and Cytokines Expression Profile Following Inflammatory Stimuli Front Immunol, 2017-02-14;8(0):141. 2017-02-14 [PMID: 28261205] (Human)
• Brand O, Somanath S, Moermans C, Yanagisawa H, Hashimoto M, Cambier S, Markovics J, Bondesson A, Hill A, Jablons D, Wolters P, Lou J, Marks J, Baron J, Nishimura S Transforming Growth Factor-beta and Interleukin-1beta Signaling Pathways Converge on the Chemokine CCL20 Promoter. J Biol Chem, 2015-04-27;290(23):14717-28. 2015-04-27 [PMID: 25918170] (Human)
• Holden V, Lenio S, Kuick R, Ramakrishnan S, Shah Y, Bachman M Bacterial siderophores that evade or overwhelm lipocalin 2 induce hypoxia inducible factor 1alpha and proinflammatory cytokine secretion in cultured respiratory epithelial cells. Infect Immun, 2014-06-30;82(9):3826-36. 2014-06-30 [PMID: 24980968] (Human)
• Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway. PLoS ONE, 2013-12-30;8(12):e82867. 2013-12-30 [PMID: 24386121] (Human)
• Austin W. Boesch, Yifeng Zhao, Alison S. Landman, Marta Rodriguez Garcia, John V. Fahey, Charles R. Wira et al. A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions Journal of Immunological Methods 2013-11-29 [PMID: 24035708] (Human)
• Sperling T, Oldak M, Walch-Ruckheim B, Wickenhauser C, Doorbar J, Pfister H, Malejczyk M, Majewski S, Keates AC, Smola S Human Papillomavirus Type 8 Interferes with a Novel C/EBPβ-Mediated Mechanism of Keratinocyte CCL20 Chemokine Expression and Langerhans Cell Migration. PLoS Pathog., 2012-07-26;8(7):e1002833. 2012-07-26 [PMID: 22911498] (Human)
• Moyes DL, Murciano C, Runglall M, Islam A, Thavaraj S, Naglik JR Candida albicans yeast and hyphae are discriminated by MAPK signaling in vaginal epithelial cells. PLoS ONE, 2011-11-08;6(11):e26580. 2011-11-08 [PMID: 22087232] (Human)
• Zimmer A, Luce S, Gaignier F, Nony E, Naveau M, Biola-Vidamment A, Pallardy M, Van Overtvelt L, Mascarell L, Moingeon P Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae. J. Immunol., 2011-03-02;186(7):3966-76. 2011-03-02 [PMID: 21368225] (Human)
• Kim S, Lewis C, Nadel JA CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells. J. Immunol., 2011-02-07;186(6):3392-400. 2011-02-07 [PMID: 21300824] (Human)
• Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur. J. Immunol., 2009-10-01;39(10):2779-88. 2009-10-01 [PMID: 19731362] (Human)
• Kurago ZB, Lam-ubol A, Stetsenko A Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation. Head Neck Pathol, 2008-03-01;2(1):1-12. 2008-03-01 [PMID: 19603082] (Human)
• Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 2009-03-02;119(10):1424-32. 2009-03-02 [PMID: 19255340] (Human)
• Hsu LW, Chen CL, Nakano T, Lai CY, Chiang KC, Lin YC, Kao YH, Chen SH, Goto T, Sung WC, Yang CH, Cheng YF, Jawan B, Chiu KW, Goto S The role of a nuclear protein, histone H1, on signalling pathways for the maturation of dendritic cells. Clin. Exp. Immunol., 2008-04-24;152(3):576-84. 2008-04-24 [PMID: 18435805] (Human)
• Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells. FASEB J., 2008-03-25;22(8):2747-57. 2008-03-25 [PMID: 18364398] (Human)
• Sa SM, Valdez PA, Wu J The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis. J. Immunol., 2007-02-15;178(4):2229-40. 2007-02-15 [PMID: 17277128] (Human)
• Pietila TE, Veckman V, Lehtonen A, Lin R, Hiscott J, Julkunen I Multiple NF-kappaB and IFN regulatory factor family transcription factors regulate CCL19 gene expression in human monocyte-derived dendritic cells. J. Immunol., 2007-01-01;178(1):253-61. 2007-01-01 [PMID: 17182562] (Human)

FAQs for CCL20/MIP-3 alpha (DY360). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: CCL20