Human CCL20/MIP-3 alpha DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CCL20/MIP-3 alpha DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL20

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL20/MIP-3 alpha DuoSet ELISA, 15 Plate

• beta chemokine exodus-1
• Beta-chemokine exodus-1
• CC chemokine LARC
• C-C motif chemokine 20
• CCL20
• chemokine (C-C motif) ligand 20
• CKb4
• exodus-1
• LARC
• LARCLiver and activation-regulated chemokine
• MIP3 alpha
• MIP-3 alpha
• MIP-3a
• MIP-3-alpha
• MIP3AMacrophage inflammatory protein 3 alpha
• SCYA20Small-inducible cytokine A20
• small inducible cytokine subfamily A (Cys-Cys), member 20
• ST38

Background

CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.

Publications for CCL20/MIP-3 alpha (DY360)(39)

Publications using DY360

• Brunner, M;Guggisberg, D;Sprecher, M;Pastva, O;Bürki, K;Houtman, M;Kreuzer, M;Krättli, SA;Jahnke, L;Roceri, M;Bruggmann, R;Elhai, M;Maurer, B;Marti, TM;Ospelt, C;Klein, K; Pro-Inflammatory Properties of Salivary Gland-Derived Fibroblasts-Implications in Sjögren's Disease Cells 2025-04-08 [PMID: 40277884] (Human)
• Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M; Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls PloS one 2024-07-15 [PMID: 39008499] (Human)
• Chick, HM;Rees, ME;Lewis, ML;Williams, LK;Bodger, O;Harris, LG;Rushton, S;Wilkinson, TS; Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses Biomedicines 2024-03-25 [PMID: 38672079] (Human)
• Kislat, A;Olah, P;Kuchner, M;Gerber, PA;Schrader, J;Meller, S;Homey, B; The Endogenous Dual Retinoid Receptor Agonist Alitretinoin Exhibits Immunoregulatory Functions on Antigen-Presenting Cells International journal of molecular sciences 2023-06-02 [PMID: 37298605] (Human)
• H Janbazacya, J van Bergen, S Varasteh, J Garssen, G Folkerts, S Braber Repeated exposure of bronchial epithelial cells to particular matter increases allergen-induced cytokine release and permeability Cytokine, 2022-04-08;154(0):155878. 2022-04-08 [PMID: 35405483] (Human)
• P Esteves, B Allard, A Celle, I Dupin, E Maurat, O Ousova, M Thumerel, JW Dupuy, T Leste-Lass, R Marthan, PO Girodet, T Trian, P Berger Asthmatic bronchial smooth muscle increases rhinovirus replication within the bronchial epithelium Cell Reports, 2022-03-29;38(13):110571. 2022-03-29 [PMID: 35354045] (Human)
• B Grüner, L Peters, A Hillenbran, P Vo beta berg, J Schweiker, EG Rollmann, LH Rodriguez, J Blumhardt, S Burkert, P Kern, C Köhler, PT Soboslay Echinococcus multilocularis specific antibody, systemic cytokine, and chemokine levels, as well as antigen-specific cellular responses in patients with progressive, stable, and cured alveolar echinococcosis: A 10-year follow-up PloS Neglected Tropical Diseases, 2022-02-02;16(2):e0010099. 2022-02-02 [PMID: 35108275] (Human)
• R Pohl, S Feder, EM Haberl, L Rein-Fisch, TS Weiss, M Spirk, A Bruckmann, N McMullen, CJ Sinal, C Buechler Chemerin Overexpression in the Liver Protects against Inflammation in Experimental Non-Alcoholic Steatohepatitis Biomedicines, 2022-01-07;10(1):. 2022-01-07 [PMID: 35052810] (Human)
• SR Bruno, A Kumar, ZF Mark, R Chandrasek, E Nakada, N Chamberlai, B Mihavics, J Walzer, J Cahoon, AE Dixon, B Cunniff, V Anathy DRP1-Mediated Mitochondrial Fission Regulates Lung Epithelial Response to Allergen International Journal of Molecular Sciences, 2021-10-15;22(20):. 2021-10-15 [PMID: 34681784] (Human)
• MS Shaban, C Müller, C Mayr-Buro, H Weiser, J Meier-Soel, BV Albert, A Weber, U Linne, T Hain, I Babayev, N Karl, N Hofmann, S Becker, S Herold, ML Schmitz, J Ziebuhr, M Kracht Multi-level inhibition of coronavirus replication by chemical ER stress Nature Communications, 2021-09-20;12(1):5536. 2021-09-20 [PMID: 34545074] (Human)
• S Gómez-Mele, FI García-Mac, T García-Mac, V Luna-Guerr, G Montero-Pe, I Túnez-Fiña, E Paz-Rojas Amino terminal recognition by a CCR6 chemokine receptor antibody blocks CCL20 signaling and IL-17 expression via beta-arrestin BMC biotechnology, 2021-07-05;21(1):41. 2021-07-05 [PMID: 34225700] (Human)
• HK Skovdahl, S Gopalakris, TD Svendsen, AVB Granlund, I Bakke, ZG Ginbot, S Thorsvik, A Flatberg, B Sporsheim, J Ostrop, TE Mollnes, AK Sandvik, T Bruland Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration Frontiers in Pharmacology, 2021-05-13;12(0):679741. 2021-05-13 [PMID: 34054553] (Human)
• GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation Biomolecules, 2021-02-21;11(2):. 2021-02-21 [PMID: 33669910] (Human)
• H Gunawardan, T Romero, N Yao, S Heidt, A Mulder, DA Elashoff, NM Valenzuela Tissue-specific endothelial cell heterogeneity contributes to unequal inflammatory responses Scientific Reports, 2021-01-21;11(1):1949. 2021-01-21 [PMID: 33479269] (Human)
• AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation PLoS Pathog., 2020-08-18;16(8):e1008781. 2020-08-18 [PMID: 32810179] (Human)
• T Zhuang, X Yi, J Chen, P Kang, X Chen, J Chen, T Cui, Y Chang, Z Ye, Q Ni, Y Wang, P Du, B Li, L Liu, Z Jian, K Li, T Gao, S Li, C Li Intracellular virus sensor MDA5 exacerbates vitiligo by inducing the secretion of chemokines in keratinocytes under virus invasion Cell Death Dis, 2020-06-12;11(6):453. 2020-06-12 [PMID: 32532953] (Human)
• FJ Ryan, AM Ahern, RS Fitzgerald, EJ Laserna-Me, EM Power, AG Clooney, KW O'Donoghue, PJ McMurdie, S Iwai, A Crits-Chri, D Sheehan, C Moran, B Flemer, AL Zomer, A Fanning, J O'Callagha, J Walton, A Temko, W Stack, L Jackson, SA Joyce, S Melgar, TZ DeSantis, JT Bell, F Shanahan, MJ Claesson Colonic microbiota is associated with inflammation and host epigenomic alterations in inflammatory bowel disease Nat Commun, 2020-03-23;11(1):1512. 2020-03-23 [PMID: 32251296] (Human)
• JA Woznicki, P Flood, M Bustamante, P Stamou, G Moloney, A Fanning, SA Zulquernai, J McCarthy, F Shanahan, S Melgar, K Nally Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-gamma and TNF-alpha in intestinal epithelial cells Cell Death Dis, 2020-01-27;11(1):68. 2020-01-27 [PMID: 31988296] (Human)
• D Martin-Gar, C Silva-Vilc, R Will, AH Enk, AS Lonsdorf Tumor-derived CCL20 affects B16 melanoma growth in mice J. Dermatol. Sci., 2019-12-13;0(0):. 2019-12-13 [PMID: 31883833] (Human)
• S Di Vincenz, IH Heijink, JA Noordhoek, C Cipollina, L Siena, A Bruno, M Ferraro, DS Postma, M Gjomarkaj, E Pace SIRT1/FoxO3 axis alteration leads to aberrant immune responses in bronchial epithelial cells J. Cell. Mol. Med., 2018-02-07;0(0):. 2018-02-07 [PMID: 29411515] (Human)
• JS Hähnlein, TH Ramwadhdoe, JF Semmelink, IY Choi, FH Berger, M Maas, DM Gerlag, PP Tak, TBH Geijtenbee, LGM van Baarse Distinctive expression of T cell guiding molecules in human autoimmune lymph node stromal cells upon TLR3 triggering Sci Rep, 2018-01-29;8(1):1736. 2018-01-29 [PMID: 29379035] (Human)
• Ewa Kuca-Warna, Weronika Kurowska, Monika Prochorec, Anna Radzikows, Tomasz Burakowsk, Urszula Skalska, Magdalena Massalska, Magdalena Pleba?czy, Barbara Ma?dyk-No, Iwona S?owi?ska, Robert Gasik, W?odzimierz Ma?li?ski Rheumatoid arthritis bone marrow environment supports Th17 response. Arthritis Research & Therapy, 2017-12-08;0(0):1478-6362. 2017-12-08 [PMID: 29216915] (Human)
• Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo IL-27 Modulates Chemokine Production in TNF-? -Stimulated Human Oral Epithelial Cells Cell. Physiol. Biochem., 2017-10-05;43(3):1198-1206. 2017-10-05 [PMID: 28977795] (Human)
• P Severino, DT Palomino, H Alvarenga, CB Almeida, DC Pasqualim, A Cury, PR Salvalaggi, AL De Vasconc, MC Andrade, T Aloia, S Bromberg, LV Rizzo, FA Rocha, LC Marti Human Lymph Node-Derived Fibroblastic and Double-Negative Reticular Cells Alter Their Chemokines and Cytokines Expression Profile Following Inflammatory Stimuli Front Immunol, 2017-02-14;8(0):141. 2017-02-14 [PMID: 28261205] (Human)
• Brand O, Somanath S, Moermans C, Yanagisawa H, Hashimoto M, Cambier S, Markovics J, Bondesson A, Hill A, Jablons D, Wolters P, Lou J, Marks J, Baron J, Nishimura S Transforming Growth Factor-beta and Interleukin-1beta Signaling Pathways Converge on the Chemokine CCL20 Promoter. J Biol Chem, 2015-04-27;290(23):14717-28. 2015-04-27 [PMID: 25918170] (Human)
• Holden V, Lenio S, Kuick R, Ramakrishnan S, Shah Y, Bachman M Bacterial siderophores that evade or overwhelm lipocalin 2 induce hypoxia inducible factor 1alpha and proinflammatory cytokine secretion in cultured respiratory epithelial cells. Infect Immun, 2014-06-30;82(9):3826-36. 2014-06-30 [PMID: 24980968] (Human)
• Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway. PLoS ONE, 2013-12-30;8(12):e82867. 2013-12-30 [PMID: 24386121] (Human)
• Austin W. Boesch, Yifeng Zhao, Alison S. Landman, Marta Rodriguez Garcia, John V. Fahey, Charles R. Wira et al. A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions Journal of Immunological Methods 2013-11-29 [PMID: 24035708] (Human)
• Sperling T, Oldak M, Walch-Ruckheim B, Wickenhauser C, Doorbar J, Pfister H, Malejczyk M, Majewski S, Keates AC, Smola S Human Papillomavirus Type 8 Interferes with a Novel C/EBPβ-Mediated Mechanism of Keratinocyte CCL20 Chemokine Expression and Langerhans Cell Migration. PLoS Pathog., 2012-07-26;8(7):e1002833. 2012-07-26 [PMID: 22911498] (Human)
• Moyes DL, Murciano C, Runglall M, Islam A, Thavaraj S, Naglik JR Candida albicans yeast and hyphae are discriminated by MAPK signaling in vaginal epithelial cells. PLoS ONE, 2011-11-08;6(11):e26580. 2011-11-08 [PMID: 22087232] (Human)
• Zimmer A, Luce S, Gaignier F, Nony E, Naveau M, Biola-Vidamment A, Pallardy M, Van Overtvelt L, Mascarell L, Moingeon P Identification of a New Phenotype of Tolerogenic Human Dendritic Cells Induced by Fungal Proteases from Aspergillus oryzae. J. Immunol., 2011-03-02;186(7):3966-76. 2011-03-02 [PMID: 21368225] (Human)
• Kim S, Lewis C, Nadel JA CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells. J. Immunol., 2011-02-07;186(6):3392-400. 2011-02-07 [PMID: 21300824] (Human)
• Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur. J. Immunol., 2009-10-01;39(10):2779-88. 2009-10-01 [PMID: 19731362] (Human)
• Kurago ZB, Lam-ubol A, Stetsenko A Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation. Head Neck Pathol, 2008-03-01;2(1):1-12. 2008-03-01 [PMID: 19603082] (Human)
• Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 2009-03-02;119(10):1424-32. 2009-03-02 [PMID: 19255340] (Human)
• Hsu LW, Chen CL, Nakano T, Lai CY, Chiang KC, Lin YC, Kao YH, Chen SH, Goto T, Sung WC, Yang CH, Cheng YF, Jawan B, Chiu KW, Goto S The role of a nuclear protein, histone H1, on signalling pathways for the maturation of dendritic cells. Clin. Exp. Immunol., 2008-04-24;152(3):576-84. 2008-04-24 [PMID: 18435805] (Human)
• Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells. FASEB J., 2008-03-25;22(8):2747-57. 2008-03-25 [PMID: 18364398] (Human)
• Sa SM, Valdez PA, Wu J The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis. J. Immunol., 2007-02-15;178(4):2229-40. 2007-02-15 [PMID: 17277128] (Human)
• Pietila TE, Veckman V, Lehtonen A, Lin R, Hiscott J, Julkunen I Multiple NF-kappaB and IFN regulatory factor family transcription factors regulate CCL19 gene expression in human monocyte-derived dendritic cells. J. Immunol., 2007-01-01;178(1):253-61. 2007-01-01 [PMID: 17182562] (Human)

FAQs for CCL20/MIP-3 alpha (DY360). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL20