Human Angiopoietin-like 4 DuoSet ELISA, 15 Plate

Levels of ANGPTL4 in sera from RA patients and controls after correction for the false positive reactions to rheumatoid factor (RF).(A) ANGPTL4 was measured in sera from 57 patients with rheumatoid arthritis (RA) and 43 matched controls by ELISA using DY3485 kit with lot IV detection antibody. (B) ANGPTL4 was measured as in (A) in sera from RF+ (n = 33) and RF- (n = 20) patients with RA. The means ± SD are indicated. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28107351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Levels of ANGPTL4 in sera from RA patients and controls after correction for the false positive reactions to rheumatoid factor (RF).(A) ANGPTL4 was measured in sera from 57 patients with rheumatoid arthritis (RA) and 43 matched controls by ELISA using DY3485 kit with lot IV detection antibody. (B) ANGPTL4 was measured as in (A) in sera from RF+ (n = 33) and RF- (n = 20) patients with RA. The means ± SD are indicated. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28107351), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Angiopoietin-like 4 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ANGPTL4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Angiopoietin-like 4 DuoSet ELISA, 15 Plate

• Angiopoietin like Protein 4
• angiopoietin-like 4
• Angiopoietin-like Protein 4
• ANGPTL4
• ARP4fasting-induced adipose factor
• FIAF
• FIAFhepatic angiopoietin-related protein
• Hepatic fibrinogen/angiopoietin-related protein
• HFARP
• HFARPANGPTL2
• NL2
• peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein
• PGAR
• PGARangiopoietin-related protein 4
• pp1158
• PPARG angiopoietin related protein

Background

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Publications for Angiopoietin-like Protein 4/ANGPTL4 (DY3485)(39)

Publications using DY3485

• Yen, IW;Chen, SC;Lin, CH;Fan, KC;Yang, CY;Hsu, CY;Kuo, CH;Lin, MS;Lyu, YP;Juan, HC;Heng-Huei, L;Li, HY; Precision medicine in diabetes prediction: Exploring a subgroup-specific biomarker strategy for risk stratification Journal of diabetes investigation 2024-11-13 [PMID: 39535373] (Human)
• Seidel, F;Vreeken, D;Custers, E;Wiesmann, M;Özsezen, S;van Duyvenvoorde, W;Caspers, M;Menke, A;Morrison, MC;Verschuren, L;Duering, M;Hazebroek, EJ;Kiliaan, AJ;Kleemann, R; Metabolic dysfunction-associated steatotic liver disease is associated with effects on cerebral perfusion and white matter integrity Heliyon 2024-10-15 [PMID: 39391513] (Human)
• Chaube, B;Citrin, KM;Sahraei, M;Singh, AK;de Urturi, DS;Ding, W;Pierce, RW;Raaisa, R;Cardone, R;Kibbey, R;Fernández-Hernando, C;Suárez, Y; Suppression of angiopoietin-like 4 reprograms endothelial cell metabolism and inhibits angiogenesis Nature communications 2023-12-12 [PMID: 38086791] (Human)
• Shen, CJ;Chan, RH;Lin, BW;Li, NC;Huang, YH;Chang, WC;Chen, BK; Oleic acid-induced metastasis of KRAS/p53-mutant colorectal cancer relies on concurrent KRAS activation and IL-8 expression bypassing EGFR activation Theranostics 2023-08-21 [PMID: 37649607] (Human)
• Kjølle, S;Finne, K;Birkeland, E;Ardawatia, V;Winge, I;Aziz, S;Knutsvik, G;Wik, E;Paulo, JA;Vethe, H;Kleftogiannis, D;Akslen, LA; Hypoxia induced responses are reflected in the stromal proteome of breast cancer Nature communications 2023-06-22 [PMID: 37349288] (Human)
• Hefni, E;Menon, D;Ma, T;Asiedu, EB;Sultan, A;Meiller, T;Schneider, A;Sodhi, A;Montaner, S; Angiopoietin-like 4 induces head and neck squamous cell carcinoma cell migration through the NRP1/ABL1/PXN pathway Cellular signalling 2023-05-09 [PMID: 37169211] (Human)
• P Bajwa, K Kordylewic, A Bilecz, RR Lastra, K Wroblewski, Y Rinkevich, E Lengyel, HA Kenny Cancer-associated mesothelial cell-derived ANGPTL4 and STC1 promote the early steps of ovarian cancer metastasis JCI Insight, 2023-03-22;0(0):. 2023-03-22 [PMID: 36795484] (Human)
• C Guo, M Deshpande, Y Niu, I Kachwala, M Flores-Bel, H Megarity, T Nuse, S Babapoor-F, M Ramada, J Sanchez, N Inamdar, TV Johnson, MV Canto-Sole, S Montaner, A Sodhi HIF-1alpha accumulation in response to transient hypoglycemia may worsen diabetic eye disease Cell Reports, 2023-01-10;42(1):111976. 2023-01-10 [PMID: 36640318] (Human)
• M Górecka, K Krzemi?ski, T Mikulski, AW Ziemba ANGPTL4, IL-6 and TNF-alpha as regulators of lipid metabolism during a marathon run Scientific Reports, 2022-11-19;12(1):19940. 2022-11-19 [PMID: 36402848] (Human)
• M Qaddoumi, P Hebbar, M Abu-Farha, A Al Somaly, M Melhem, F Al-Kayal, I AlKhairi, P Cherian, M Alanbaei, F Al-Mulla, J Abubaker, TA Thanaraj GALNT2 rs4846914 SNP Is Associated with Obesity, Atherogenic Lipid Traits, and ANGPTL3 Plasma Level Genes, 2022-07-04;13(7):. 2022-07-04 [PMID: 35885984] (Human)
• Y Qin, A Dinabandhu, X Cao, JC Sanchez, K Jee, M Rodrigues, C Guo, J Zhang, J Vancel, D Menon, NS Khan, T Ma, SY Tzeng, YJ Daoud, JJ Green, GL Semenza, S Montaner, A Sodhi ANGPTL4 influences the therapeutic response of neovascular age-related macular degeneration patients by promoting choroidal neovascularization JCI Insight, 2022-07-08;0(0):. 2022-07-08 [PMID: 35653189] (Human)
• Y Katanasaka, A Saito, Y Sunagawa, N Sari, M Funamoto, S Shimizu, K Shimizu, T Akimoto, C Ueki, M Kitano, K Hasegawa, G Sakaguchi, T Morimoto ANGPTL4 Expression Is Increased in Epicardial Adipose Tissue of Patients with Coronary Artery Disease Journal of Clinical Medicine, 2022-04-27;11(9):. 2022-04-27 [PMID: 35566578] (Human)
• T Sun, W Zhan, L Wei, Z Xu, L Fan, Y Zhuo, C Wang, J Zhang Circulating ANGPTL3 and ANGPTL4 levels predict coronary artery atherosclerosis severity Lipids in Health and Disease, 2021-11-06;20(1):154. 2021-11-06 [PMID: 34742313] (Human)
• M Li, J Hu, L Yao, M Gao Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure J. Cell. Mol. Med., 2020-08-03;0(0):. 2020-08-03 [PMID: 32745373] (Human)
• PMM Ruppert, CCJR Michielsen, EJ Hazebroek, A Pirayesh, G Olivecrona, LA Afman, S Kersten Fasting induces ANGPTL4 and reduces LPL activity in human adipose tissue Mol Metab, 2020-06-03;40(0):101033. 2020-06-03 [PMID: 32504883] (Human)
• Yan Q. Chen, Thomas G. Pottanat, Robert W. Siegel, Mariam Ehsani, Yue-Wei Qian, Eugene Y. Zhen et al. Angiopoietin-like protein 8 differentially regulates ANGPTL3 and ANGPTL4 during postprandial partitioning of fatty acids[S] Journal of Lipid Research 2020-08-01 [PMID: 32487544] (Human)
• F Vendrame, L Olops, STO Saad, FF Costa, KY Fertrin Hypocholesterolemia and dysregulated production of angiopoietin-like proteins in sickle cell anemia patients Cytokine, 2019-05-01;120(0):88-91. 2019-05-01 [PMID: 31054480] (Human)
• C Pich, P Meylan, B Mastelic-G, NT Nguyen, R Loyon, BK Trang, H Moser, C Moret, C Goepfert, J Hafner, MP Levesque, P Romero, C Jandus, L Michalik Induction of paracrine signaling in metastatic melanoma cells by PPAR? agonist rosiglitazone activates stromal cells and enhances tumor growth Cancer Res., 2018-09-05;0(0):. 2018-09-05 [PMID: 30185551] (Human)
• V Gusarova, C O'Dushlain, TM Teslovich, PN Benotti, T Mirshahi, O Gottesman, CV Van Hout, MF Murray, A Mahajan, JB Nielsen, L Fritsche, AB Wulff, DF Gudbjartss, M Sjögren, CA Emdin, RA Scott, WJ Lee, A Small, LC Kwee, OP Dwivedi, RB Prasad, S Bruse, AE Lopez, J Penn, A Marcketta, JB Leader, CD Still, HL Kirchner, UL Mirshahi, AH Wardeh, CM Hartle, L Habegger, SN Fetterolf, T Tusie-Luna, AP Morris, H Holm, V Steinthors, P Sulem, U Thorsteins, JI Rotter, LM Chuang, S Damrauer, D Birtwell, CM Brummett, AV Khera, P Natarajan, M Orho-Melan, J Flannick, LA Lotta, CJ Willer, OL Holmen, MD Ritchie, DH Ledbetter, AJ Murphy, IB Borecki, JG Reid, JD Overton, O Hansson, L Groop, SH Shah, WE Kraus, DJ Rader, YI Chen, K Hveem, NJ Wareham, S Kathiresan, O Melander, K Stefansson, BG Nordestgaa, A Tybjærg-Ha, GR Abecasis, D Altshuler, JC Florez, M Boehnke, MI McCarthy, GD Yancopoulo, DJ Carey, AR Shuldiner, A Baras, FE Dewey, J Gromada Genetic inactivation of ANGPTL4 improves glucose homeostasis and is associated with reduced risk of diabetes Nat Commun, 2018-06-13;9(1):2252. 2018-06-13 [PMID: 29899519] (Human)
• T Zhang, A Kastrenopo, Q Larrouture, NA Athanasou, HJ Knowles Angiopoietin-like 4 promotes osteosarcoma cell proliferation and migration and stimulates osteoclastogenesis BMC Cancer, 2018-05-08;18(1):536. 2018-05-08 [PMID: 29739381] (Human)
• Q Meng, Y Qin, M Deshpande, F Kashiwabuc, M Rodrigues, Q Lu, H Ren, JH Elisseeff, GL Semenza, SV Montaner, A Sodhi Hypoxia-Inducible Factor-Dependent Expression of Angiopoietin-Like 4 by Conjunctival Epithelial Cells Promotes the Angiogenic Phenotype of Pterygia Invest. Ophthalmol. Vis. Sci., 2017-09-01;58(11):4514-4523. 2017-09-01 [PMID: 28873177] (Human)
• K Jee, M Rodrigues, F Kashiwabuc, BP Applewhite, I Han, G Lutty, MF Goldberg, GL Semenza, S Montaner, A Sodhi Expression of the angiogenic mediator, angiopoietin-like 4, in the eyes of patients with proliferative sickle retinopathy PLoS ONE, 2017-08-23;12(8):e0183320. 2017-08-23 [PMID: 28832635] (Human)
• AS El-Shal, HE Zidan, NM Rashad, FM Wadea Angiopoietin-like protein 3 and 4 expression 4 and their serum levels in hepatocellular carcinoma Cytokine, 2017-03-31;96(0):75-86. 2017-03-31 [PMID: 28371666] (Human)
• H Okamoto, K Cavino, E Na, E Krumm, S Kim, PE Stevis, J Harp, AJ Murphy, GD Yancopoulo, J Gromada Angptl4 does not control hyperglucagonemia or �-cell hyperplasia following glucagon receptor inhibition Proc. Natl. Acad. Sci. U.S.A, 2017-01-31;0(0):. 2017-01-31 [PMID: 28143927] (Human)
• T Casselli, H Qureshi, E Peterson, D Perley, E Blake, B Jokinen, A Abbas, S Nechaev, JA Watt, A Dhasarathy, CA Brissette MicroRNA and mRNA Transcriptome Profiling in Primary Human Astrocytes Infected with Borrelia burgdorferi PLoS ONE, 2017-01-30;12(1):e0170961. 2017-01-30 [PMID: 28135303] (Human)
• E Makoveichu, T Ruge, S Nilsson, A S”dergren, G Olivecrona High Concentrations of Angiopoietin-Like Protein 4 Detected in Serum from Patients with Rheumatoid Arthritis Can Be Explained by Non-Specific Antibody Reactivity PLoS ONE, 2017-01-20;12(1):e0168922. 2017-01-20 [PMID: 28107351] (Human)
• S Janciauski Angiopoietin-like protein 4 and cardiovascular function in COPD BMJ Open Respir Res, 2016-10-31;3(1):e000161. 2016-10-31 [PMID: 27933182] (Human)
• Frenzel E, Wrenger S, Brugger B, Salipalli S, Immenschuh S, Aggarwal N, Lichtinghagen R, Mahadeva R, Marcondes A, Dinarello C, Welte T, Janciauskiene S alpha1-Antitrypsin Combines with Plasma Fatty Acids and Induces Angiopoietin-like Protein 4 Expression. J Immunol, 2015-09-11;195(8):3605-16. 2015-09-11 [PMID: 26363050] (Human)
• Mousavizadeh R, Khosravi S, Behzad H, McCormack R, Duronio V, Scott A Cyclic strain alters the expression and release of angiogenic factors by human tendon cells. PLoS ONE, 2014-05-13;9(5):e97356. 2014-05-13 [PMID: 24824595] (Human)
• Frenzel E, Wrenger S, Immenschuh S, Koczulla R, Mahadeva R, Deeg H, Dinarello C, Welte T, Marcondes A, Janciauskiene S Acute-phase protein alpha1-antitrypsin--a novel regulator of angiopoietin-like protein 4 transcription and secretion. J Immunol, 2014-04-23;192(11):5354-62. 2014-04-23 [PMID: 24760148] (Human)
• Mathieu M, Iampietro M, Chuchana P, Guerit D, Djouad F, Noel D, Jorgensen C Involvement of angiopoietin-like 4 in matrix remodeling during chondrogenic differentiation of mesenchymal stem cells. J Biol Chem, 2014-02-06;289(12):8402-12. 2014-02-06 [PMID: 24505142] (Human)
• Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study. BMC Infect Dis, 2014-01-20;14(0):35. 2014-01-20 [PMID: 24444080] (Human)
• Ferguson B, Gao X, Zelazowski M, Lee J, Jeter C, Abba M, Aldaz C The cancer gene WWOX behaves as an inhibitor of SMAD3 transcriptional activity via direct binding. BMC Cancer, 2013-12-11;13(0):593. 2013-12-11 [PMID: 24330518] (Human)
• Clement L, Mace C, Avila-Casado C, Joles J, Kersten S, Chugh S Circulating angiopoietin-like 4 links proteinuria with hypertriglyceridemia in nephrotic syndrome. Nat Med, 2013-12-08;20(1):37-46. 2013-12-08 [PMID: 24317117] (Human)
• Katanasaka Y, Kodera Y, Kitamura Y, Morimoto T, Tamura T, Koizumi F Epidermal growth factor receptor variant type III markedly accelerates angiogenesis and tumor growth via inducing c-myc mediated angiopoietin-like 4 expression in malignant glioma. Mol Cancer, 2013-04-25;12(0):31. 2013-04-25 [PMID: 23617883] (Human)
• Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma. J. Exp. Med., 2012-10-01;209(11):1985-2000. 2012-10-01 [PMID: 23027923] (Human)
• Makoveichuk E, Sukonina V, Kroupa O, Thulin P, Ehrenborg E, Olivecrona T, Olivecrona G Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4 are formed. Biochem Biophys Res Commun, 2012-07-20;425(2):138-43. 2012-07-20 [PMID: 22820186] (Human)
• Knowles HJ, Cleton-Jansen AM, Korsching E, Athanasou NA Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4. FASEB J., 2010-07-28;24(12):4648-59. 2010-07-28 [PMID: 20667978] (Human)
• Ma T, Jham BC, Hu J Viral G protein-coupled receptor up-regulates Angiopoietin-like 4 promoting angiogenesis and vascular permeability in Kaposi's sarcoma. Proc. Natl. Acad. Sci. U.S.A., 2010-07-26;107(32):14363-8. 2010-07-26 [PMID: 20660728] (Human)

FAQs for Angiopoietin-like Protein 4/ANGPTL4 (DY3485). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ANGPTL4