HTRA1/PRSS11 Antibody (275603) [Unconjugated]

HTRA1/PRSS11 was detected in immersion fixed paraffin-embedded sections of human fibrolamellar hepatocellular carcinoma and IBS intestine (positive staining; upper panels) and human cervical squamous cell carcinoma and smooth muscle (negative staining; lower panels) using Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB29161) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane (luminal surface of intestinal gland). View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 275603
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HTRA1/PRSS11 Antibody (275603) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HTRA1/PRSS11
  Gln23-Pro480
  Accession # Q92743
• Specificity: Detects human HTRA1/PRSS11 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HTRA1/PRSS11 Antibody (275603) [Unconjugated]

• ARMD7
• EC 3.4.21
• EC 3.4.21.108
• High-temperature requirement A serine peptidase 1
• HtrA serine peptidase 1
• HTRA1
• IGFBP5-protease
• L56
• ORF480
• PRSS11
• Serine protease 11
• serine, 11 (IGF binding)

Background

HTRA1 is a serine protease encoded by the PRSS11 gene and is expressed in many tissues. HTRA1 regulates the availability of IGFs by cleaving IGFBPs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q92743()