HspA1B Antibody (HSPA1B/7623)

Formalin-fixed, paraffin-embedded human testis stained with HspA1B antibody (HSPA1B/7623). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Western blot analysis of T47D & HEK-293 cell lysates using HspA1B antibody (HSPA1B/7623).

• Reactivity: Hu
• Applications: WB, IHC
• Clone: HSPA1B/7623
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

HspA1B Antibody (HSPA1B/7623) Summary

• Description: Store at 2 to 8C. Non-hazardous. No MSDS required.
• Immunogen: Recombinant fragment (around aa400-600) of human HspA1B protein (exact sequence is proprietary)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA1B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2ug/ml
  • Western Blot 1-2ug/ml
• Application Notes: Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for HspA1B Antibody (HSPA1B/7623)

• FLJ54328
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1B
• heat shock 70kDa protein 1B
• HSP70.1/HSP70.2
• HSP70-1/HSP70-2
• HSP70-1B
• HSP70-2
• HSPA1
• HSPA1A

Background

HSPA1B encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA1B