Recombinant Human HSP90 beta Protein

SDS-Page: Recombinant Human HSP90 beta Protein [NBP3-18320] - SDS-PAGE of 90kDa HSP90 beta protein (NBP3-18320). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HSP90 beta protein (NBP3-18320).

• Reactivity: Hu
• Applications: WB, ELISA, IP, PAGE, Bioactivity, SPR

Recombinant Human HSP90 beta Protein Summary

• Description: A biologically active protein corresponding to human HSP90 beta.

  Source: Sf 9 (baculovirus)

  Amino Acid Sequence: MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD

• Localization: Cytoplasm|Melanosome
• Preparation Method: This protein is affinity purified.
• Protein/Peptide Type: Recombinant Protein
• Gene: HSP90AB1
• Purity: >85%
• Endotoxin Note: Low Endotoxin

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • ELISA
  • Immunoprecipitation
  • SDS-Page
  • Surface Plasmon Resonance
  • Western Blot
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 20mM Tris, pH7.5, 175 mM NaCl, 0.1 mM EDTA, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Purity: >85%

Alternate Names for Recombinant Human HSP90 beta Protein

• D6S182
• Heat shock 84 kDa
• heat shock 90kD protein 1, beta
• heat shock 90kDa protein 1, beta
• heat shock protein 90kDa alpha (cytosolic), class B member 1
• heat shock protein beta
• heat shock protein HSP 90-beta
• HSP 84
• HSP84
• HSP90 beta
• HSP90AB1
• HSP90B
• HSP90-BETA
• HSP90BHSP 90
• HSPC2
• HSPC2D6S182
• HSPCB
• HSPCBFLJ26984

Background

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HSP90AB1