HSP90 beta Antibody (SY46-01)

Western Blot: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Analysis of Hsp90 beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate Lane 3: Jurkat cell lysate Lane 4: 293 cell lysate

Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Staining Hsp90 beta in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Analysis of paraffin-embedded mouse testis tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.

Flow Cytometry: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Flow cytometric analysis of HSP90 beta was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Staining Hsp90 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Staining Hsp90 beta in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Analysis of paraffin-embedded human colon cancer tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Analysis of paraffin-embedded human liver cancer tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Analysis of paraffin-embedded human tonsil tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP90 beta Antibody (SY46-01) [NBP2-67395] - Analysis of paraffin-embedded mouse brain tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin.

Product Discontinued

HSP90 beta Antibody (SY46-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human HSP90 beta aa 485-724 / 724. (SwissProt: P08238 Human; SwissProt: P11499 Mouse; SwissProt: P34058 Rat)
• Localization: Cytoplasm, Melanosome,Nucleus,Secreted,Cell membrane,Dynein axonemal particle .
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSP90AB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Simple Western
  • Western Blot 1:1000-1:10000
• Application Notes: Use in SW reported in scientific publication PMID: 32443895

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HSP90 beta Antibody (SY46-01)

• D6S182
• Heat shock 84 kDa
• heat shock 90kD protein 1, beta
• heat shock 90kDa protein 1, beta
• heat shock protein 90kDa alpha (cytosolic), class B member 1
• heat shock protein beta
• heat shock protein HSP 90-beta
• HSP 84
• HSP84
• HSP90 beta
• HSP90AB1
• HSP90B
• HSP90-BETA
• HSP90BHSP 90
• HSPC2
• HSPC2D6S182
• HSPCB
• HSPCBFLJ26984

Background

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP90 beta Antibody (NBP2-67395)(2)

Publications using NBP2-67395

• Haney MJ, Zhao Y, Jin YS, Batrakova EV Extracellular Vesicles as Drug Carriers for Enzyme Replacement Therapy to Treat CLN2 Batten Disease: Optimization of Drug Administration Routes Cells 2020-05-20 [PMID: 32443895] (Simple Western, Mouse)
• Haney, MJ;Klyachko, NL;Harrison, EB;Zhao, Y;Kabanov, AV;Batrakova, EV; TPP1 Delivery to Lysosomes with Extracellular Vesicles and their Enhanced Brain Distribution in the Animal Model of Batten Disease Adv Healthc Mater 2019-04-18 [PMID: 30997751] (WB, Mouse)

Bioinformatics

• Gene Symbol: HSP90AB1