HSP90 beta Antibody (341346) [Unconjugated]

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.25 µg/mL Mouse Anti-Human/Mouse/Rat HSP90 beta Monoclonal Antibody (Catalog # MAB32861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HSP90a and HSP90 beta (5 ng/lane) were included. A specific band for HSP90 beta was detected at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and HSP90 beta knockout HEK293T cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat HSP90 beta Monoclonal Antibody (Catalog # MAB32861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP90 beta at approximately 90 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clone: 341346
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HSP90 beta Antibody (341346) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HSP90 beta
  Met1-Asp724
  Accession # P08238
• Specificity: Detects human, mouse, and rat HSP90 beta in Western blots and direct ELISAs. In Western blots and direct ELISAs, no cross-reactivity with recombinant human HSP90 alpha is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSP90AB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HSP90 beta Antibody (341346) [Unconjugated]

• D6S182
• Heat shock 84 kDa
• heat shock 90kD protein 1, beta
• heat shock 90kDa protein 1, beta
• heat shock protein 90kDa alpha (cytosolic), class B member 1
• heat shock protein beta
• heat shock protein HSP 90-beta
• HSP 84
• HSP84
• HSP90 beta
• HSP90AB1
• HSP90B
• HSP90-BETA
• HSP90BHSP 90
• HSPC2
• HSPC2D6S182
• HSPCB
• HSPCBFLJ26984

Background

HSP90 is a composite name for a large group of Heat Shock Proteins whose molecular weights average 90 kDa. HSPs function primarily as a molecular chaperone, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP90 is a central component in a number of basic cellular processes including hormone signaling and cell cycle control. HSP90 is ubiquitously expressed, highly conserved and accounts for 1-2% of the total cellular protein. Recently introduced, standardized nomenclature has divided the 17 identified HSP90 genes into three related and one unrelated classes, HSP90AA, HSP90AB, HSP90BB, and TRAP, respectively. Eukaryotic cells have two principal isoforms of HSP90. The antibody described here reacts with the constitutively expressed, 724 amino acid, HSP90AB1-isoform 1 (also known as HSP90 beta , HSP90B, HSPCB, HSPC2, and HSP89 beta ). The inducible form, HSP90AA1, is a 737 amino acid protein (also known as HSP90 alpha , HSP90A, HSPC1, HSPCA, HSP89 alpha and LAP2). HSP90AB1-1 and HSP90AA share 90% identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP90 beta Antibody (MAB32861)(2)

Publications using MAB32861

• Mali GR, YeYati PL, Mizuno S et al. ZMYND10 functions in a chaperone relay during axonemal dynein assembly. Elife. 6/19/2018 [PMID: 29916806]
• Kasioulis I, Syred HM, Tate P et al. Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis. Mol. Biol. Cell. 2014-02-19 [PMID: 24554764]

Bioinformatics

• Gene Symbol: HSP90AB1
• Uniprot:
  • P08238()