HSP90 alpha Antibody - BSA Free

Western Blot: HSP90 alpha Antibody [NBP1-77685] - Analysis of HSP901a in 1: HeLa. 2: Ntera2. 3: A431. 4: HepG2. 5: MCF7. 6: NIH/3T3. 7: PC12. 8: COS7.

Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77685] - HSP901a antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).

Immunohistochemistry: HSP90 alpha Antibody [NBP1-77685] - Staining of human kidney carcinoma in HSP901A using DAB with hematoxylin counterstain.

Simple Western: HSP90 alpha Antibody [NBP1-77685] - Image shows a specific band for Hsp90A in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.15 mg/ml

HSP90 alpha Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human Hsp90A protein (between residues 650-732) [UniProt P07900]
• Localization: Cytoplasm. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSP90AA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:500
  • Western Blot 2 ug/mL
• Application Notes: In Western blot a band is seen at ~84 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  A-431 Whole Cell Lysate
  HeLa Whole Cell Lysate
  NTERA-2 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.15 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HSP90 alpha Antibody - BSA Free

• FLJ31884
• Heat shock 86 kDa
• heat shock 90kD protein 1, alpha
• heat shock 90kD protein 1, alpha-like 4
• heat shock 90kD protein, alpha-like 4
• heat shock 90kDa protein 1, alpha
• heat shock protein 90kDa alpha (cytosolic), class A member 1
• heat shock protein HSP 90-alpha
• HSP86
• Hsp89
• HSP89A
• HSP90 alpha
• Hsp90
• HSP90A
• HSP90AA1
• HSP90AHSP86
• HSP90N
• HSPC1
• HSPC1HSP 86
• HSPCA
• HSPCAHSP89A
• HSPCAL1
• HSPCAL4
• HSPN
• LAP2
• Renal carcinoma antigen NY-REN-38

Background

Heat shock protein 90-1a (HSP90-1a) is a myosin chaperone involved in heat shock responses, as well as in myofibrillogenesis. It is known to interact with histone methyltransferases, influencing epigenetic changes in certain pathways. It interacts with TERT to assemble the telomerase holoenzyme complex, which is critical for addition of DNA to telomeric ends. It also is important in thick and thin filament organization, influencing the formation and maintenance of sarcomere characteristics. It is highly expressed in skeletal and cardiac muscles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSP90AA1
• Entrez:
  • 3320(Human)
  • 15519(Mouse)
  • 299331(Rat)
• Uniprot:
  • P07900()
  • P07901(Mouse)
  • P82995(Rat)