HSP70/HSPA1A Antibody (BB70) - BSA Free

Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (NB110-96427). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96427) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.

Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (NB110-96427). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96427) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSPA1A cells stained brown. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

Western Blot: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.

Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Detection in paraffin embedded liver sections and nuclear sections of rat hepatocytes by staining with BB70. First pictures in series show two hours after exposure to stress, the second shows the control.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Courtesy of: G. Matic, University of Belgrade, Serbia.

Immunohistochemistry: HSP70/HSPA1A Antibody (BB70) [NB110-96427] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (NB110-96427). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96427) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSPA1A stained brown. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Fi, Gp, Ha, Rb, Sh, Xp, Ye
• Applications: WB, ICC/IF, IHC, IP
• Clone: BB70
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HSP70/HSPA1A Antibody (BB70) - BSA Free Summary

• Immunogen: Chicken HSP70/HSP90 complex
• Localization: Cytoplasm
• Specificity: Detects approx 72 (HSP) and approx 73kDa (HSC).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of HSP70/HSC70 Antibody was sufficient for detection of HSP70 and HSC70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HSP70/HSPA1A Antibody (BB70) - BSA Free

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport..

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP70/HSPA1A Antibody (NB110-96427)(5)

Publications using NB110-96427

• Roths M, Rudolph TE, Krishna S et al. One day of environment-induced heat stress damages the murine myocardium American Journal of Physiology - Heart and Circulatory Physiology 2024-10-01 [PMID: 39212770]
• Roths M, Freestone A, Rudolph T et al. Environment-induced heat stress causes structural and biochemical changes in the heart Journal of Thermal Biology 2023-04-01 [PMID: 37055111]
• Rudolph TE, Roach CM, Baumgard LH et al. The impact of Zearalenone on heat-stressed skeletal muscle in pigs Journal of Animal Science 2022-08-01 [PMID: 35908787] (Immunohistochemistry, Canine)
• Adur MK, Seibert JT, Romoser MR et al. Porcine endometrial heat shock proteins are differentially influenced by pregnancy status, heat stress, and altrenogest supplementation during the peri-implantation period Journal of animal science 2022-07-01 [PMID: 35772767] (Immunohistochemistry, Canine)
• Seibert, J T, Adur, M K Et al. Differentiating between the effects of heat stress and lipopolysaccharide on the porcine ovarian heat shock protein response1. J Anim Sci 2019-12-17 [PMID: 31782954] (IF/IHC, Canine)
  
  Details:
  Citation using the Allophycocyanin/Cy7 format of this antibody.

Review for HSP70/HSPA1A Antibody (NB110-96427) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Pig.

reviewed by: Verified Customer WB Pig 06/30/2017

Summary

• Application: Western Blot
• Sample Tested: Skeletal muscle tissue
• Species: Pig

Bioinformatics

• Gene Symbol: HSPA1A
• Entrez:
  • 423504(Chicken)
  • 24472(Rat)
• Uniprot:
  • P08106(Chicken)