HSP70/HSPA1A Antibody (242707) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with a 42 °C heat shock for 30 minutes with a 3 hour recovery. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP70/HSPA1A at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

HSP70/HSPA1A was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) by heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

KNK437 inhibition assay of cell cultures. (A) Viability test results of KNK437 (10, 50 and 100 μM) inhibition assay of mononuclear peripheral blood burst formation unit-erythroid (BFU-E) cultures, with and without EPO. (B) Viability test results of a KNK437 (50 and 100 μM) inhibition assay of CD34+ bone marrow BFU-E cultures, with and without EPO. (C) Viability test results of KNK437 treatment (50 μM) on HEL and Ba/F3 JAK2 V617F (BAF3MUT) cell lines. X axis: concentration (μM); Y axis: % of viability cells (viability test) or BFU-E (BFU-E cultures) (D) Molecular effects of KNK437 in HEL cell line Western blot of HSP90, HSP70, p-JAK2 (decreased with KNK437 treatment), p-ERK and p-P38. Actin was used as the housekeeping control. (E) Molecular effects of KNK437 in Ba/F3 cell line Western blot of JAK2, p-ERK, ERK, p-STAT5, STAT5 AND Actin, (F) Western blot of HSP90, HSP70 , JAK2, STAT5 and p-STAT5. Expression levels of HSP70, JAK2 and p-STAT5 decreased after HSP70 siRNA interference on HEL cell line. Actin was used as the housekeeping control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24252366), licensed under a CC-BY license. Not internally tested by R&D Systems.

KNK437 inhibition assay of cell cultures. (A) Viability test results of KNK437 (10, 50 and 100 μM) inhibition assay of mononuclear peripheral blood burst formation unit-erythroid (BFU-E) cultures, with and without EPO. (B) Viability test results of a KNK437 (50 and 100 μM) inhibition assay of CD34+ bone marrow BFU-E cultures, with and without EPO. (C) Viability test results of KNK437 treatment (50 μM) on HEL and Ba/F3 JAK2 V617F (BAF3MUT) cell lines. X axis: concentration (μM); Y axis: % of viability cells (viability test) or BFU-E (BFU-E cultures) (D) Molecular effects of KNK437 in HEL cell line Western blot of HSP90, HSP70, p-JAK2 (decreased with KNK437 treatment), p-ERK and p-P38. Actin was used as the housekeeping control. (E) Molecular effects of KNK437 in Ba/F3 cell line Western blot of JAK2, p-ERK, ERK, p-STAT5, STAT5 AND Actin, (F) Western blot of HSP90, HSP70 , JAK2, STAT5 and p-STAT5. Expression levels of HSP70, JAK2 and p-STAT5 decreased after HSP70 siRNA interference on HEL cell line. Actin was used as the housekeeping control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24252366), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clone: 242707
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HSP70/HSPA1A Antibody (242707) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HSP70/HSPA1A
  Met1-Asp641
  Accession # NP_005336
• Specificity: Detects the induced form of human and mouse HSP70/HSPA1A in Western blots. In Western blots, no cross-reactivity with the constitutively expressed HSC70 (HSP73) is detected.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA1A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 0.5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HSP70/HSPA1A Antibody (242707) [Unconjugated]

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP70/HSPA1A Antibody (MAB1663)(17)

Publications using MAB1663

• Kim, R;Kim, JH; Engineered Extracellular Vesicles with Compound-Induced Cargo Delivery to Solid Tumors International journal of molecular sciences 2023-05-27 [PMID: 37298320] (Western Blot, Primate - Chlorocebus aethiops (African Green Monkey))
• Anna Nickelsen, Claudia Götz, Florian Lenz, Karsten Niefind, Simone König, Joachim Jose Analyzing the interactome of human CK2 beta in prostate carcinoma cells reveals HSP70 ‐1 and Rho guanin nucleotide exchange factor 12 as novel interaction partners FASEB BioAdvances 2023-03-01 [PMID: 36876296] (Western Blot, Human)
• MF Pittenger, S Eghtesad, PG Sanchez, X Liu, Z Wu, L Chen, BP Griffith MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted Hearts International Journal of Molecular Sciences, 2022-01-08;23(2):. 2022-01-08 [PMID: 35054878] (Western Blot, Rat)
• R Sonntag, C Penners, M Kohlhepp, U Haas, D Lambertz, A Kroh, T Cramer, F Ticconi, IG Costa, F Tacke, N Gassler, C Trautwein, C Liedtke Cyclin E1 in Murine and Human Liver Cancer: A Promising Target for Therapeutic Intervention during Tumour Progression Cancers, 2021-11-13;13(22):. 2021-11-13 [PMID: 34830835] (Western Blot, Human)
• I Szczuka, J Wierzbicki, P Serek, BM Szcz??niak, M Krzystek-K Heat Shock Proteins HSPA1 and HSP90AA1 Are Upregulated in Colorectal Polyps and Can Be Targeted in Cancer Cells by Anti-Inflammatory Oxicams with Arylpiperazine Pharmacophore and Benzoyl Moiety Substitutions at Thiazine Ring Biomolecules, 2021-10-27;11(11):. 2021-10-27 [PMID: 34827586] (Western Blot, Human)
• Fabian Freisleben, Franziska Modemann, Jana Muschhammer, Hauke Stamm, Franziska Brauneck, Alexander Krispien et al. Mebendazole Mediates Proteasomal Degradation of GLI Transcription Factors in Acute Myeloid Leukemia International Journal of Molecular Sciences 2021-10-01 [PMID: 34639011]
• Vincent Deruelle, Stéphanie Bouillot, Viviana Job, Emmanuel Taillebourg, Marie-Odile Fauvarque, Ina Attrée et al. The bacterial toxin ExoU requires a host trafficking chaperone for transportation and to induce necrosis Nature Communications 6/29/2021 [PMID: 34188051]
• Nik Mohamed Kamal NNS, Awang RAR, Mohamad S, Shahidan WNS. Plasma- and Saliva Exosome Profile Reveals a Distinct MicroRNA Signature in Chronic Periodontitis Frontiers in Physiology 2020-11-30 [PMID: 33329037]
• BH Chen, YJ Chang, S Lin, WY Yang Hsc70/Stub1 promotes the removal of individual oxidatively stressed peroxisomes Nat Commun, 2020-10-19;11(1):5267. 2020-10-19 [PMID: 33077711] (Western Blot, Mouse)
• K Dissanayak, M Nõmm, F Lättekivi, Y Ressaissi, K Godakumara, A Lavrits, G Midekessa, J Viil, R Bæk, MM Jørgensen, S Bhattachar, A Andronowsk, A Salumets, Ü Jaakma, A Fazeli Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers Theriogenology, 2020-04-04;149(0):104-116. 2020-04-04 [PMID: 32259747] (Microarray, Mouse)
• Lucia D’Amico, Ulrike Menzel, Michael Prummer, Philipp Müller, Mélanie Buchi, Abhishek Kashyap et al. A novel anti-HER2 anthracycline-based antibody-drug conjugate induces adaptive anti-tumor immunity and potentiates PD-1 blockade in breast cancer Journal for ImmunoTherapy of Cancer 2019-12-01 [PMID: 30665463] (Flow Cytometry, Immunocytochemistry, Human)
• Y Takamura, W Aoki, A Satomura, S Shibasaki, M Ueda Small RNAs detected in exosomes derived from the MH7A synovial fibroblast cell line with TNF-? stimulation PLoS ONE, 2018-08-10;13(8):e0201851. 8/10/2018 [PMID: 30096164]
• Guangxin Li Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori Am J Transl Res, 2016-10-15;8(10):4184-4194. 2016-10-15 [PMID: 27830002] (Western Blot, Rat)
• R-F Mao, V Rubio, H Chen, L Bai, O C Mansour, Z-Z Shi OLA1 protects cells in heat shock by stabilizing HSP70 Cell Death & Disease 2013-02-14 [PMID: 23412384]
• JENNIFER L. LIEDEL, YUEE GUO, YUEYUE YU, SHENG-RU SHIOU, SANGZI CHEN, ELAINE O. PETROF et al. Motherʼs Milk-Induced Hsp70 Expression Preserves Intestinal Epithelial Barrier Function in an Immature Rat Pup Model Pediatric Research 2011-05-01 [PMID: 21263375]
• Vanessa Fétaud-Lapierre, Catherine M. Pastor, Annarita Farina, Denis F. Hochstrasser, Jean-Louis Frossard, Pierre Lescuyer Proteomic Analysis of Heat Shock-Induced Protection in Acute Pancreatitis Journal of Proteome Research 2010-11-05 [PMID: 20815342]
• Bhatnagar S, Shinagawa K, Castellino FJ, Schorey JS Exosomes released from macrophages infected with intracellular pathogens stimulate a proinflammatory response in vitro and in vivo. Blood, 2007-07-31;110(9):3234-44. 2007-07-31 [PMID: 17666571] (Western Blot, Human)

Bioinformatics

• Gene Symbol: HSPA1A
• Uniprot:
  • NP_005336()