HSP60 Antibody (NB100-56469)

Images

 
Western Blot: Hsp60 Antibody [NB100-56469] - analysis of Hsp60 in the 1) absence and 2) presence of imminizing peptide in HeLa cell lysate and the 3) absence and 4) presence of immunizing peptide in Jurkat cell lysate ...read more

Product Details

Summary
Reactivity Hu, Bv, Ca, Ch, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HSP60 Antibody Summary

Immunogen
A portion of amino acids 350-400 of human Hsp60 was used as the immunogen.
Marker
Mitochondria Marker
Specificity
The amino acid sequence used as immunogen is 100% homologous in a broad range of species including human, dog, mouse, rat, bovine, chicken, chimp, monkey, and orangutan.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPD1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2-1 ug/ml
Positive Control
Jurkat Lysate (NB800-PC2)
HeLa Lysate (NB800-PC1)
HSP60 Lysate (NBL1-11766)

Reactivity Notes

Cross reacts with Bovine, Chicken, Chimpanzee, Dog, Human and New Word Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for HSP60 Antibody

  • 60 kDa chaperonin
  • Chaperonin 60
  • cpn60
  • GROEL
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock 60kDa protein 1 (chaperonin)
  • Heat shock protein 60
  • heat shock protein 65
  • HLD4
  • HSP60
  • HSP-60
  • HSP60SPG13
  • HSP65
  • HSPD1
  • HuCHA60
  • Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial
  • P60 lymphocyte protein
  • short heat shock protein 60 Hsp60s1
  • spastic paraplegia 13 (autosomal dominant)
  • SPG13

Background

HSP60, a 60KDa member of Chaperonin family, is a mitochondrial protein that participates in protein folding, transport and secretion. HSP60 is a multi-functional protein whose roles include accelerating the maturation of pro-caspase 3 during apoptosis, LPS-binding, acting as a ligand for innate immune system and also binding to the surface of innate immune cells and stimulating proinflammatory reactivity. It induces the expression of IL-12 and IL-15 and also induces release of TNF-alpha, NO and IL-6. Reports suggest interaction of Hsp60 with integrin molecules during metastasis. Mutations in Hsp60 gene cause autosomal recessive spastic paraplegia 13. Hsp60 has been found to be involved in development of auto-immune disorders such as Rheumatoid arthritis, insulin-dependent diabetes mellitus, systemic sclerosis and atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-11473
Species: Hu, Mu, Rt, Po, Bv, Ca, GP, Rb, Sh, Xp
Applications: WB, ELISA, IHC, IHC-P, IP
NBP1-81696
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-02459
Species: Hu, Mu, Rt, Ch, Dr, Pm
Applications: WB, ELISA, IHC, IHC-P
NB120-2928
Species: Hu, Mu, Rt, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-32972
Species: Hu, Mu, Rt, Ch, Pm, Sh
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Flow-CS, Flow-IC
NBP1-97868
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Mk, Rb, Sh
Applications: WB, EM, EIA, Flow, ICC/IF, IHC, IHC-P, IP
H00010049-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-02996
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-87148
Species: Hu
Applications: WB, IHC, IHC-P
AF1640
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ELISA(Sta)
AF4309
Species: Hu, Mu
Applications: WB, IHC, ICC
Supplier Logo

Publications for HSP60 Antibody (NB100-56469) (0)

There are no publications for HSP60 Antibody (NB100-56469).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HSP60 Antibody (NB100-56469) (0)

There are no reviews for HSP60 Antibody (NB100-56469). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HSP60 Antibody (NB100-56469) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional HSP60 Products

Related Products by Gene

Bioinformatics Tool for HSP60 Antibody (NB100-56469)

Discover related pathways, diseases and genes to HSP60 Antibody (NB100-56469). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HSP60 Antibody (NB100-56469)

Discover more about diseases related to HSP60 Antibody (NB100-56469).
 

Pathways for HSP60 Antibody (NB100-56469)

View related products by pathway.

PTMs for HSP60 Antibody (NB100-56469)

Learn more about PTMs related to HSP60 Antibody (NB100-56469).
 

Research Areas for HSP60 Antibody (NB100-56469)

Find related products by research area.

Blogs on HSP60.

TLR4 - A Guardian of Innate Immunity
Toll-like receptor 4 (TLR4) belongs to the family of Toll-like receptors (TLR), and plays a main role in pathogen recognition and innate immunity system activation. The TLR family members are highly conserved proteins that all contain a high degree of...  Read full blog post.

Customer Experience using HSP60 Antibody
I began using the HSP60 antibody (NB110-57063) in June of 2010 and it worked well. I do not like to buy antibodies that have not been tested in the species for which I will use them, so I picked this antibody because it had already been tested in rat...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HSP60 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol HSPD1
Entrez
Uniprot