HSD3B1 Antibody

Product Discontinued

HSD3B1 Antibody Summary

• Immunogen: Peptide with sequence C-DRHKETLKSKTQ corresponding to C-Terminus according to NP_000853.1.
• Marker: Trophoblast-associated Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HSD3B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.003-0.01 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human placenta lysates (calculated MW of 42.3 kDa band according to NP_000853.1). In transfected HEK293 transiently expressing human HSD3B1 a band of approx. 45 kDa is observed. This band is not observed in the non-transfected HEK293.
  IHC: Paraffin embedded Human Placenta.
• Control:

  HSD3B1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HSD3B1 Antibody

• 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
• 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I
• 3-beta-HSD I
• 3BETAHSD
• 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
• 3BH
• delta-5-3-ketosteroid isomerase
• EC 1.1.1
• HSD3B
• HSDB3
• HSDB3A
• hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
• hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1
• I
• progesterone reductase
• SDR11E1
• short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase
• steroid Delta-isomerase
• Trophoblast antigen FDO161G

Background

3beta-hsd is a bifunctional enzyme, that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3beta-hsd enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSD3B1 Antibody (NB600-982)(1)

Publication using NB600-982

• Chang BL, Zheng SL, Hawkins GA et al. Joint effect of HSD3B1 and HSD3B2 genes is associated with hereditary and sporadic prostate cancer susceptibility. Cancer Res 2002-03-15 [PMID: 11912155]

Bioinformatics

• Gene Symbol: HSD3B1
• Entrez:
  • 3283(Human)
• Uniprot:
  • NP_000853.1(Human)