HSD17B4 Antibody - BSA Free

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining in human liver and pancreas tissues using anti-HSD17B4 antibody. Corresponding HSD17B4 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human liver, lung, lymph node and testis using Anti-HSD17B4 antibody NBP1-85297 (A) shows similar protein distribution across tissues to independent antibody NBP1-85296 (B).

Western Blot: HSD17B4 Antibody [NBP1-85297] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Immunocytochemistry/Immunofluorescence: HSD17B4 Antibody [NBP1-85297] - Staining of human cell line U-2 OS shows localization to peroxisomes.

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human lung.

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human liver shows high expression.

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human lymph node.

Immunohistochemistry-Paraffin: HSD17B4 Antibody [NBP1-85297] - Staining of human testis.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HSD17B4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HSD17B4 Antibody - BSA Free (NBP1-85297) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-HSD17B4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LRFDGRVVLVTGAGAGLGRAYALAFAERGALVVVNDLGGDFKGVGKGSLAADKVVEEIRRRGGKAVANYDSVEEGEKVVKTALDAFG
• Predicted Species: Mouse (90%), Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HSD17B4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  HSD17B4 Protein (NBP1-85297PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for HSD17B4 Antibody - BSA Free

• 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase
• 7-alpha
• D-bifunctional protein
• EDH17B4
• hydroxysteroid (17-beta) dehydrogenase 4
• MPF-2
• peroxisomal multifunctional enzyme type 2,17-beta-HSD IV
• peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV
• peroxisomal
• short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Background

The protein encoded by the HSD17B4 gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathwayfor fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chainand 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidationactivity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present onchromosome 8. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSD17B4 Antibody (NBP1-85297)(1)

Publication using NBP1-85297

• Sophonnithiprasert T, Aruksakunwong O, Tashiro E et al. Interaction between goniothalamin and peroxisomal multifunctional enzyme type 2 triggering endoplasmic reticulum stress Heliyon 2020-10-01 [PMID: 33102840] (WB, Human)

Bioinformatics

• Gene Symbol: HSD17B4